Analysis of urinary N-acetyl-β-glucosaminidase by capillary zone electrophoresis

Friedberg, Michael A.; Shihabi, Zak K.

doi:10.1016/s0378-4347(97)00135-7

Cited by 8 publications

(3 citation statements)

References 8 publications

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“…The problems involved in analyzing these compounds by CE have been reviewed by El Rassi and Mechef [126] and by Olechno and Dolan [12]. Other papers by Jin et al [128], by Easley et al [129], or by Friedberg and Shihabi [130] describe exclusively CZE methods with either UV or fluorescence detection for the screening of urinary carbohydrates or to determine the activity of N-acetyl-b-glucosamidase (NAG), an early marker for renal damage in the urine, and will not be discussed in this section. Zamfir et al [131] have indicated MS as an option for the identification of O-glycosilated amino acids and glycopeptides in the urine of patients suffering from N-acetylhexosaminidase deficiency.…”

Section: Carbohydratesmentioning

confidence: 99%

Micellar electrokinetic chromatographic and capillary zone electrophoretic methods for screening urinary biomarkers of human disorders: A critical review of the state‐of‐the‐art

et al. 2005

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Human urine plays a central role in clinical diagnostic being one of the most-frequently used body fluid for detection of biological markers. Samples from patients with different diseases display patterns of biomarkers that differ significantly from those obtained from healthy subjects. The availability of fast, reproducible, and easy-to-apply analytical techniques that would allow identification of a large number of these analytes is thus highly desiderable since they may provide detailed information about the progression of a pathological process. From among the variety of methods so far applied for the determination of urinary metabolites, capillary electrophoresis, both in the capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) modes, represents a robust and reliable analytical tool widely used in this area. The aim of the present article is to focus the interest of the reader on recent applications of MEKC and CZE in the field of urinary biomarkers and to discuss advantages and/or limitations of each mode.

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Section: Carbohydratesmentioning

confidence: 99%

Micellar electrokinetic chromatographic and capillary zone electrophoretic methods for screening urinary biomarkers of human disorders: A critical review of the state‐of‐the‐art

et al. 2005

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“…14 Capillary electrophoresis has been used to separate the reaction product 4-methyl-umbelliferone from the reaction mixture, followed by its detection by UV absorbance or fluorimetry. 15 Enzyme immunoassays for the NAGase iso-zymes have also been developed using monoclonal antibodies specific for either of the A or B forms. 16 There appear to be no published reports of NAGase assay methods employing electrochemical detection.…”

Section: Introductionmentioning

confidence: 99%

An assay for the enzyme N-acetyl-Î²-d-glucosaminidase (NAGase) based on electrochemical detection using screen-printed carbon electrodes (SPCEs)

Pemberton

Hart

Mottram

2001

Analyst

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An electrochemical assay for the enzyme N-acetyl-beta-D-glucosaminidase (NAGase) is described, using bare screen-printed carbon electrodes (SPCEs). The enzyme substrate, 1-naphthyl-N-acetyl-beta-D-glucosaminide, was added to the NAGase-containing sample under hydrodynamic conditions and was hydrolysed to 1-naphthol, which was monitored amperometrically at an Eapp of +650 mV versus SCE. A pH study revealed the apparent Vmax for the assay to occur at pH 4.5. corresponding to an apparent substrate Km of 0.28 mM. In order to be compatible with the analysis of biological fluids, a final operating pH of 5.4 was selected, and, using a data recording time of 100 s post-substrate addition, the assay gave a linear response (r2 = 0.988) over the range 3.1 to 108 mU ml(-1) NAGase (RSD = 15.4%). This assay has the potential to monitor NAGase levels in a number of application areas.

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“…CZE method correlated well with an automated kinetic fluorescent assay. An example is analysis of NAG by CE after incubation of urine samples using synthetic substrate, methylumbelliferyl-β-d-glucosaminide [37].…”

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Advancement in Analytical and Bioanalytical Techniques as a Boon to Medical Sciences

Pandey¹,

Mishra²

2020

Biochemical Testing - Clinical Correlation and Diagnosis

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The most important objectives frequently found in analytical and bioanalytical chemistry involve advancement of analytical techniques and its application to relevant medical/clinical problems. Keeping in view to these aspects, the present chapter is primarily focused on the development of advanced analytical techniques applied in the medical field. For example, N-acetyl-beta-D-glucosaminidase (NAG) enzyme is a specific biomarker of acute kidney injury. A biomarker is an entity that is purposely measured and estimated as an indicator of normal biological process, pathogenic process, or pharmacological responses to a therapeutic intervention. Hence, successive measurements of urinary NAG may enhance its clinical use as an indicator of ongoing tubular injury. Hence, in order to obtain information for selective monitoring of biomarker, the development of a practical and valid analytical method is important. Experimentation is driven by the need to know more about the medical effects and safety features of the biologically active analyte. It is therefore more important to evaluate the information that is already available for that particular analyte and to quantify the level of uncertainty for the proposed technique.

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Analysis of urinary N-acetyl-β-glucosaminidase by capillary zone electrophoresis

Cited by 8 publications

References 8 publications

Micellar electrokinetic chromatographic and capillary zone electrophoretic methods for screening urinary biomarkers of human disorders: A critical review of the state‐of‐the‐art

Micellar electrokinetic chromatographic and capillary zone electrophoretic methods for screening urinary biomarkers of human disorders: A critical review of the state‐of‐the‐art

An assay for the enzyme N-acetyl-Î²-d-glucosaminidase (NAGase) based on electrochemical detection using screen-printed carbon electrodes (SPCEs)

Advancement in Analytical and Bioanalytical Techniques as a Boon to Medical Sciences

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