Analysis of urinary aromatic acids by liquid chromatography tandem mass spectrometry

Abstract

The separation and detection of 11 urinary aromatic acids was developed using HPLC-MS/MS. The method features a simple sample preparation involving a single-step dilution with internal standard and a rapid 8 min chromatographic separation. The accuracy was evaluated by the recovery of known spikes between 87 and 110%. Inter- and intra-assay precision (CV) was below 11% in all cases and the analytes were observed to be stable for up to 8 weeks when stored at -20 degrees C. The method was validated based upon linearity, accuracy, precision and stability and was used to establish reference intervals for children and adults.

Metabotypes Related to Meat and Vegetable Intake Reflect Microbial, Lipid and Amino Acid Metabolism in Healthy People

“…Excretion of PGA may be indicative of changes in glutathione requirements, and/or deficient glutathione resynthesis . Epidemiological studies link high consumption of meat with increased risk of colorectal cancer, cardiovascular disease, and type 2 diabetes, and the finding that 2‐hydroxybutyrate and PAG are elevated even in apparently healthy meat eaters suggests that this type of diet may be leading to alterations in glutathione metabolism due to increased oxidative stress and inflammation . Follow‐up work should investigate potential links with the ‘`meat metabotype'’ and inflammation.…”

Simultaneous determination of urinary quinolinate, gentisate, 4-hydroxybenzoate, and α-ketoglutarate by high-performance liquid chromatography-tandem mass spectrometry

“…[38][39][40] Specically, a Synergi POLAR-RP (4.0 mm, 2.0 Â 150 mm, Phenomenex, USA), a Luna phenyl-hexyl (3.0 mm, 3.0 Â 150 mm, Phenomenex, USA), and a Synergi Fusion-RP column (4.0 mm, 3.0 Â 150 mm, Phenomenex, USA) were examined for their ability to effectively retain and separate the selected RCC biomarkers. The potential for interferences is heightened in RCC patients who may suffer from impaired renal function, leading to clearance of physiologically retained biomolecules.…”

Effects of systemic administration of kynurenic acid and glycine on renal haemodynamics and excretion in normotensive and spontaneously hypertensive rats

“…Concentration of KYNA in human urine was estimated to be 4-40 μM (Zhao et al 2011;Crow et al 2008;Hiratsuka et al 2012).…”