Analysis of Ultrasound Fields in Cell Culture Wells for In Vitro Ultrasound Therapy Experiments

Hensel, Karin; Mienkina, Martin P.; Schmitz, Georg

doi:10.1016/j.ultrasmedbio.2011.09.007

Cited by 99 publications

(87 citation statements)

References 25 publications

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“…3 and 4) in the present study, DU-145 cells treated with ultrasound with microbubbles exhibited an increase in Beclin-1 expression, which favored autophagy (Fig. 2) An increasing number of studies on the biological effect of ultrasound have indicated that the effects of ultrasound reach far beyond its classic role in diagnosis (17)(18)(19)(20)(21)(22). Ultrasound therapy may be divided into different types according to different frequencies.…”

Section: Discussionsupporting

confidence: 56%

Upregulation of Beclin-1 expression in DU-145 cells following low-frequency ultrasound irradiation combined with microbubbles

et al. 2015

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Abstract. Castration-resistant prostate cancer (PCa) is difficult to treat. Autophagy, which is an evolutionarily conserved mechanism, plays an important role in cancer development. The balance between cell death and survival in different stages varies in cancer development. The role of autophagy in PCa development has not yet been fully elucidated. Ultrasound may be of value in the treatment of PCa. The aim of the present study was to investigate the association between autophagy and ultrasound combined with microbubbles. The MTT assay was used to evaluate cell viability. Autophagy was observed by transmission electron microscopy. Reverse transcription-polymerase chain reaction and western blot analysis were used to assess the expression of autophagy-related genes. The results revealed that cell viability was significantly reduced by ultrasound combined with microbubbles in DU145 PCa cells. The present study demonstrated that ultrasound combined with microbubbles induced autophagy and autophagy-related DU-145 cell death. Notably, these findings highlighted additional mechanisms that suggest the potential of ultrasound-modulated autophagy as a novel therapeutic strategy for PCa.

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Section: Discussionsupporting

confidence: 56%

Upregulation of Beclin-1 expression in DU-145 cells following low-frequency ultrasound irradiation combined with microbubbles

et al. 2015

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“…The vast majority of experimental setups used for in vitro investigations on the effects of ultrasound on cells (including sonoporation) however have been carried out in plastic well plates [15][16][17][18]49]. These substrates are problematic from a variety of perspectives including generating reflections and heat [17].…”

Section: Discussionmentioning

confidence: 99%

“…These substrates are problematic from a variety of perspectives including generating reflections and heat [17]. The generation of standing waves are especially problematic in these setups [18,49]. Ultimately, any of these factors -and especially the presence of more than one -can create unwanted bioeffects as well as make it difficult to ensure that the designated level of energy of the treatments is being provided to the cells [17,18,49].…”

Section: Discussionmentioning

confidence: 99%

Development of a custom biological scaffold for investigating ultrasound-mediated intracellular delivery

Bui

Aleid

Alassaf

et al. 2017

Materials Science and Engineering: C

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“…In some cases, the transducer is positioned perpendicularly to the cell support, and the occurrence of multiple reflections and ultimately standing waves is unavoidable. 153 Hensel et al 154 have also demonstrated that the position of the transducer with respect to the sample holders could greatly influence the acoustic field. They concluded on the necessity to use acoustically transparent materials, e.g., a polymer presenting an acoustic impedance close to that of water.…”

Section: Acoustic Excitationmentioning

confidence: 99%

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

et al. 2016

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Besides their use as contrast agents for ultrasound imaging, microbubbles are increasingly studied for a wide range of therapeutic applications. In particular, their ability to enhance the uptake of drugs through the permeabilization of tissues and cell membranes shows great promise. In order to fully understand the numerous paths by which bubbles can interact with cells and the even larger number of possible biological responses from the cells, thorough and extensive work is necessary. In this review, we consider the range of experimental techniques implemented in in vitro studies with the aim of elucidating these microbubble-cell interactions. First of all, the variety of cell types and cell models available are discussed, emphasizing the need for more and more complex models replicating in vivo conditions together with experimental challenges associated with this increased complexity. Second, the different types of stabilized microbubbles and more recently developed droplets and particles are presented, followed by their acoustic or optical excitation methods. Finally, the techniques exploited to study the microbubble-cell interactions are reviewed. These techniques operate over a wide range of timescales, or even off-line, revealing particular aspects or subsequent effects of these interactions. Therefore, knowledge obtained from several techniques must be combined to elucidate the underlying processes. V C 2016 AIP Publishing LLC.

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Analysis of Ultrasound Fields in Cell Culture Wells for In Vitro Ultrasound Therapy Experiments

Cited by 99 publications

References 25 publications

Upregulation of Beclin-1 expression in DU-145 cells following low-frequency ultrasound irradiation combined with microbubbles

Upregulation of Beclin-1 expression in DU-145 cells following low-frequency ultrasound irradiation combined with microbubbles

Development of a custom biological scaffold for investigating ultrasound-mediated intracellular delivery

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

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