Analysis of two D1-like dopamine receptors from the honey bee Apis mellifera reveals agonist-independent activity

Mustard, Julie A.; Blenau, Wolfgang; Hamilton, Ingrid S.; Ward, Vernon K.; Ebert, Paul R.; Mercer, Alison R.

doi:10.1016/s0169-328x(03)00091-3

Cited by 91 publications

(121 citation statements)

References 42 publications

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“…Earlier reports have shown that dopamine receptor densities (24), levels of dopamine receptor gene transcript (25,26), and patterns of dopamine receptor gene expression in the brain (25,27) change during the lifetime of the adult worker bee, and differential microarray analysis has tentatively identified dopamine receptor genes among several hundred genes proposed to be modulated by QMP (28). We used Northern blot analysis and quantitative RT-PCR (see Materials and Methods) to resolve whether levels of dopamine receptor gene expression are influenced by this pheromone.…”

Section: Resultsmentioning

confidence: 99%

“…Next, we asked whether QMP-induced changes affect the responsiveness of brain tissues to dopamine. Because dopamine receptor activation alters intracellular cAMP levels (25,26,29,30), measurements of intracellular cAMP were used to monitor tissue responses to this amine (see Materials and Methods). We chose to examine dopamine-evoked responses in mushroom body calyces because these structures can be easily isolated from the brain.…”

Section: Resultsmentioning

confidence: 99%

“…We chose to examine dopamine-evoked responses in mushroom body calyces because these structures can be easily isolated from the brain. Moreover, intrinsic mushroom body neurons (Kenyon cells) express all three of the dopamine receptor genes identified in honey bees, Amdop1, Amdop2, and Amdop3 (25,26,29,30), and mushroom bodies receive extensive input from dopamine-immunoreactive neurons (31).…”

Section: Resultsmentioning

confidence: 99%

“…In some QMP-treated bees, cAMP levels fell slightly in response to dopamine application, suggesting that a D2-like dopamine receptor, such as AmDOP3, is present also in these tissues. In contrast with the actions of AmDOP1 and AmDOP2 receptors (26,29), activation of Am-DOP3 receptors reduces intracellular levels of cAMP (30).…”

Section: Discussionmentioning

confidence: 99%

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Queen pheromone modulates brain dopamine function in worker honey bees

Beggs

Glendining²,

Marechal³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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153

139

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Honey bee queens produce a sophisticated array of chemical signals (pheromones) that influence both the behavior and physiology of their nest mates. Most striking are the effects of queen mandibular pheromone (QMP), a chemical blend that induces young workers to feed and groom the queen and primes bees to perform colony-related tasks. But how does this pheromone operate at the cellular level? This study reveals that QMP has profound effects on dopamine pathways in the brain, pathways that play a central role in behavioral regulation and motor control. In young worker bees, dopamine levels, levels of dopamine receptor gene expression, and cellular responses to this amine are all affected by QMP. We identify homovanillyl alcohol as a key contributor to these effects and provide evidence linking QMPinduced changes in the brain to changes at a behavioral level. This study offers exciting insights into the mechanisms through which QMP operates and a deeper understanding of the queen's ability to regulate the behavior of her offspring.Apis mellifera ͉ biogenic amines ͉ neuroethology ͉ neuromodulation ͉ pheromonal communication C omplex social interactions require systems of communication that are reliable and unambiguous. The honey bee, Apis mellifera, employs Ͼ50 substances to communicate with and to organize its colony, and the information that is conveyed between colony members is both subtle and sophisticated (1-3).Maintaining colony organization is a primary role of the queen, whose pheromones enable her to regulate not only the behavior but also the physiology of her nest mates. The most striking effects are those of queen mandibular pheromone (QMP), a chemical blend that induces young workers to feed and groom the queen (Fig. 1) and primes bees to perform colonyrelated tasks (4, 5). The retinue of workers that attend the queen facilitates the distribution of QMP throughout the colony, where it inhibits the rearing of new queens (6), helps prevent the development of worker ovaries (7), influences comb-building activities, (8) and affects the biosynthesis of juvenile hormone (9) [and hence the age-related behavioral ontogeny of recipient workers (10)]. Despite QMP's central role in the normal functioning and organization of honey bee colonies, very little is known about the cellular mechanisms through which it operates.We were intrigued by one of the key components of QMP, homovanillyl alcohol (HVA) (4). HVA (4-hydroxy-3-methoxyphenylethanol) bears a striking structural resemblance to dopamine (see Fig. 1 Right), a biogenic amine that plays a central role in insect behavioral regulation and motor control (11-18). The presence of this compound within the pheromone blend suggested to us that dopamine function in the brain of recipient bees might be affected by exposure to QMP. To test this hypothesis, we exposed young workers to QMP and examined its effects on brain dopamine levels, levels of dopamine receptor gene expression, and cellular responses to this amine. The results provide compelling evidence that QMP al...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Queen pheromone modulates brain dopamine function in worker honey bees

Beggs

Glendining²,

Marechal³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

153

139

View full text Add to dashboard Cite

show abstract

“…There are three arthropod DARs: two type-1 receptors and one type-2 receptor (Gotzes et al, 1994;Feng et al, 1996;Han et al, 1996;Hearn et al, 2002;Blenau et al, 1998;Beggs et al, 2005;Mustard et al, 2003). A fourth arthropod receptor that responds to DA with an increase in cAMP has been cloned, but it is primarily activated by ecdysteroids and does not appear to belong to the DAR family (Srivastava et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Arthropod D2 receptors positively couple with cAMP through the Gi/o protein family

Clark

Baro

2007

Comparative Biochemistry and Physiology Part B: Biochemistry an

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The pyloric network is an important model system for understanding neuromodulation of rhythmic motor behaviors like breathing or walking. Dopamine (DA) differentially modulates neurons within the pyloric network. However, while the electrophysiological actions of DA have been well characterized, nothing is known about the signaling events that mediate its effects. We have begun a molecular characterization of DA receptors (DARs) in this invertebrate system. Here, we describe the cloning and characterization of the lobster D 2 receptor, D 2αPan . We found that when expressed in HEK cells, the D 2αPan receptor is activated by DA, but not other monoamines endogenous to the lobster nervous system. This receptor positively couples with cAMP through multiple Gi/o proteins via two discrete pathways: 1) a Gα mediated inhibition of adenylyl cyclase (AC), leading to a decrease in cAMP and 2) a Gβγ mediated activation of Phospholipase Cβ (PLCβ), leading to an increase in cAMP. Alternate splicing alters the potency and efficacy of the receptor, but does not affect monoamine specificity. Finally, we show that arthropod D 2 receptor coupling with cAMP varies with the cellular milieu.

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Molecular biology of the invertebrate dopamine receptors

Mustard

Beggs

Mercer

2005

Arch Insect Biochem Physiol

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102

132

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Dopamine is found in the nervous systems of both vertebrates and invertebrates. However, the specific actions of dopamine depend on the dopamine receptor type that is expressed in the target cell. As in mammals, different subtypes of dopamine receptors have been cloned and characterized from invertebrates, and these receptor subtypes have different structural and functional properties. Understanding how these receptors respond to dopamine and in which cells each receptor type is expressed is key to our understanding of the role of dopamine signaling. Comparison of the amino acid sequences and experimentally determined functional properties suggest that there are at least three distinct types of dopamine receptors in invertebrates. This review focuses on invertebrate dopamine receptors for which the genes have been isolated and identified, and examines our current knowledge of the functional and structural properties of these receptors, and their pharmacology and expression.

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Analysis of two D1-like dopamine receptors from the honey bee Apis mellifera reveals agonist-independent activity

Cited by 91 publications

References 42 publications

Queen pheromone modulates brain dopamine function in worker honey bees

Queen pheromone modulates brain dopamine function in worker honey bees

Arthropod D2 receptors positively couple with cAMP through the Gi/o protein family

Molecular biology of the invertebrate dopamine receptors

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