Analysis of Tunisian date palm germplasm using simple sequence repeat primers

Zehdi-Azouzi, Salwa; Sakka, Hela; Rhouma, Abdelmajid; Ould, Mohamed Salem A. Marrakchi M.; Trifi, Mokhtar

doi:10.5897/ajb2004.000-2040

Cited by 73 publications

(46 citation statements)

References 14 publications

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“…Restriction fragment length polymorphisms (RFLP) were used for date palm cultivar identification (Corniquel and Mercier, 1994), however the technique was considered laborious and unsuitable for studying large numbers of samples. Other molecular markers including random amplified polymorphic DNA (RAPD) (Sedra et al, 1998;Soliman et al, 2003;Williams et al, 1990), inter simple sequence repeats (ISSR) (Hamza et al, 2013;Hamza et al, 2012), amplified fragment length polymorphism (AFLP) (Al-Khalifah and Askari, 2003;Cao and Chao, 2002;El-assar et al, 2005;Elassar et al, 2003); simple sequence repeat (SSR) (Ahmed and AlQaradawi, 2009;Al-Ruqaishi et al, 2008;Elsafi, 2012;Elshibli and Korpelainen, 2008;Pintaud et al, 2010;Zehdi et al, 2004;Zehdi et al, 2012;Zehdi-Azouzi et al, 2015;Zehdi-Azouzi et al, 2016), chloroplast DNA analysis (Sakka et al, 2013;Rhouma-Chatti et al, 2014) and inter-primer binding site (iPBS) (Al-Najm et al, 2016) were used to assess the genetic diversity of date palm germplasm collected from a variety of countries. Other researchers used a combination of these marker systems to study the genetic diversity of date palm germplasm (Abdulla and Gamal, 2010;Adawy et al, 2005;Hussein et al, 2005;Saker et al, 2006;Saker et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Molecular variability and population structure of a core collection of date palm (Phoenix dactylifera L.) cultivars from Australia and the Middle East

Al-Najm¹,

Luo²,

Ahmad³

et al. 2017

Aust J Crop Sci

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Understanding genetic relatedness in date palm (Phoenix dactylifera L.) germplasm is important for effective plant improvement. Microsatellite (SSR) markers were used to assess the molecular variation and population structure of 60 Australian locally selected and exotic genotypes (24 female, 30 male and 6 related species), 12 Iraqi female cultivars and 10 female cultivars from Jordan. The main objectives were to survey genetic diversity and determine population structure in this core date palm collection which includes the most important and widely distributed cultivars in Australia. These Australian accessions were then compared to those originating from the date palm center of origin in the Middle East. PCR of 17 SSR primers (co-dominant markers) produced a total of 313 alleles ranging from 5 to 31 with an average of 18.4 alleles per locus. The PIC value for these 17 primers ranged from 0.4771 to 0.8199 with a mean of 0.670. The mean expected heterozygosity (0.841), mean observed heterozygosity (0.946) and Shannon's information index (2.067) indicated a high level of genetic diversity among the accessions. Multi-locus DNA fingerprints based on the 17 SSR loci unambiguously differentiated all accessions and revealed an absence of duplicated samples. Ordination and cluster analyses showed that the Australian accessions did not group together geographically; instead separate male and female groups differentiated among the six clusters. A Bayesian cluster analysis also partitioned the accessions into six groups and this result was largely compatible with the result of ordination analysis. The Australian date palm germplasm is highly diverse and thus provides an effective platform for plant improvement, enhancement of date production and the conservation of genetic resources.

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Section: Introductionmentioning

confidence: 99%

Molecular variability and population structure of a core collection of date palm (Phoenix dactylifera L.) cultivars from Australia and the Middle East

Al-Najm¹,

Luo²,

Ahmad³

et al. 2017

Aust J Crop Sci

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“…Three loci i.e. mpdCIRO78, mpdCIRO85 and mpdCIRO25 were found to be polymorphic among 49 accessions of date palm cultivars from Tunisia (Zehdi et al, 2004), while on the other hand, these markers were unable to confirm the genetic identity and true to typeness of 60 date palm accessions from Sudan (Elshibli and Korpelainen, 2008), 45 cultivars from Pakistan (Faqir et al, 2016), and 12 cultivars from Qatar (Elmeer and Mattat, 2015). Ten SSR primers (mPdCIR010,mPdCIR016,mPdCIR025,mPdCIR032,mPdCIR035,mPdCIR044,mPdCIR070,mPdCIR085,mPdCIR090 and mPdCIR093) resulted in unique fingerprints for the important genotypes of date palm from Oman (AlRuqaishi et al, 2008).…”

Section: Date Palm Diversity Evaluation Based On Simple Sequence Repementioning

confidence: 99%

Diversity Assessment and Cultivar Identification in Date Palm through Molecular Markers- A Review

Faqir

Muhammad²

2017

Turkish JAF Sci.Tech.

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Date palm has a long history of cultivation and a valuable germplasm with little knowledge about its genetic makeup and variation among the most cultivated cultivars. Diversity is the variability of a species. Plants show variation in yield, vegetative traits and morphological properties of fruits and seeds in response to environmental changes. Molecular markers or DNA markers have been in use since past three decades. The DNA profiles give information about the genotype, screen the whole genome and show variation in both the coding and noncoding region and hence give information about polymorphism. Since plastid genes are transferred mostly from the mother line, the identification of maternal lines is possible by the sequencing of plastid genes. Simple sequence repeats (SSRs) can detect length variation with the help of Polymerase Chain Reaction (PCR) and may be used as highly informative genetic markers. Single Nucleotide Polymorphism (SNPs) are the third generation of molecular markers. SNPs are more stable and have high fidelity of inheritance as compared to other marker systems. Molecular markers have been developed but they are not enough for sufficient diversity assessment. Therefore there is a need to increase the number of DNA markers in date palm. Previously, there are several studies to type various commercially important germplasm based on morphological or yield parameters. Morphological and biochemical markers are limited in number and are affected by environmental factors and growth stage of the plant which reduce their reliability in the assessment of diversity and characterization of the germplasm. This necessitates the use of genetic characterization, utilizing DNA markers, gene sequencing or SNP genotyping which can work independent of the plant growth stage and are not affected by environmental factors. A combination of morphological, biochemical and molecular characterization of the date palm cultivars can better assess the level of diversity and relationship among the cultivars.

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“…Molecular markers have to be developed for each species, from genomic regions that have undergone sufficiently rapid evolutionary change, in order to be useful for discriminating among closely-related germplasm (Cheng et al, 2002;Zehdi et al, 2004;Prasad, 2014;Daudu et al, 2016).…”

Section: Genetic Diversity Among Hibiscus Genotypes Usedmentioning

confidence: 99%

Genetic diversity among four genotypes from Hibiscus rosasinensis in relation to drought stress

Shanan¹,

Moghaieb²

2016

J. Hortic. For.

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The effect of drought stress on growth and water relations of four Hibiscus genotypes was investigated. Drought stress significantly retarded vegetative growth as analyzed by plant height, stem diameter, leaf area, fresh and dry weight. The osmotic potential ( s ) values decreased with increasing drought time, and the decrease was more pronounced in Fantasia followed by Jay´s orange. Osmotic adjustment (O.A.) increased with drought time, and was greater in Fantasia followed by Jay´s orange and lowest in Annie wood. The genetic diversity among the four hibiscus genotypes was detected by Random Amplified Polymorphic DNA (RAPD) analysis. The data indicate that, thirty-one out of fifty four RAPD markers detected were polymorphic (57.4%). The RAPD specific markers for each genotype were determined. Ten out of the thirty one polymorphic RAPD markers generated were found to be genotypespecific (32.2%). In the meantime, the largest number of RAPD genotype-specific markers was generated for primers OPE-D-07(six markers) while for both of the OPE-B-17 and OP D-05 (two markers each). These markers can be verified as being RAPD markers associated with drought tolerance in the hibiscus genotypes that could help in marker-assisted selection breeding program.

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Analysis of Tunisian date palm germplasm using simple sequence repeat primers

Cited by 73 publications

References 14 publications

Molecular variability and population structure of a core collection of date palm (Phoenix dactylifera L.) cultivars from Australia and the Middle East

Molecular variability and population structure of a core collection of date palm (Phoenix dactylifera L.) cultivars from Australia and the Middle East

Diversity Assessment and Cultivar Identification in Date Palm through Molecular Markers- A Review

Genetic diversity among four genotypes from Hibiscus rosasinensis in relation to drought stress

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