Analysis of Tryptophan and Its Metabolites by Reverse‐phase High‐pressure Liquid Chromatography

Abstract

SUMMARYRapid separation and determination of tryptophan, N^-formylkynurenine, kynurenine, 3-hydroxykynurenine, 3-hydroxyanthranilic acid, nicotinic acid, nicotinamide, trigonelline, kynurenic acid and xanthurenic acid from pea seedling using reverse-phase high-pressure liquid chromatography is described. Accuracy of the determinations was better than 5 % and less than 50 pmol of each metabolite could be measured reliably.

Biosynthesis of vitamin B3 and NAD+: incubating HepG2 cells with the alkaloid myosmine

“…6 TRP contributes possibly to formation of NA in seedlings of Zea mays, 7 and NA and NAM were detected in metabolic studies with 3 H-and 14 C-labeled TRP in the Orchidaceae species Cattleya skinneri. 8 The role of TRP and that of other potential precursors in the biosynthesis of NA and NAM in plants is still unclear in detail 9 and requires further research. In humans, TRP is predominantly provided through the diet and supplies additional precursors for serotonin, indole, tryptamine, and kynurenine biosynthesis.…”

NIACIN BIOSYNTHESIS IN LEAF DISCS AND SEEDLINGS OF CATTLEYA SKINNERI (ORCHIDACEAE)

“…Metabolites in the aqueous extracts of plants provided with tryptophan were separated at 20 + 2° C by reverse-phase high-performance liquid chromatography (Tarr and Arditti, 1981). In experiments with aspartate and glycerol the flow rate was 15 ml min~^ and the eluant was 50 mM sodium tetrabutylammonium phosphate (ion pair reagent) in 10% (v/v) aqueous methanol, pH 7-0 (Hengen, Seibert and Hengen, 1978) for the first 8 min.…”