Analysis of triglycerides by consecutive chromatographic techniques. i.cuphea llavia seed fat

Abstract

The triglycerides ofCuphea llavia var.miniata seed fat were separated according to the number of double bonds/molecule using preparative thin layer chromatography (TLC) on silicic acid impregnated with silver ion. The recovered frac‐tions were quantitated by the chromotropic acid technique. Each fraction was then separated ac‐cording to mol wt using gas‐liquid chromatog‐raphy (GLC). This multiple chromatography procedure resolvedC. llavia triglycerides into 17 different components. The triglyceride composition ofC. llavia seed fat was calculated from the above results. Since the fat contains 91.2 mole % decanoic acid, it was expected that each triglyceride molecule would contain at least two molecules of decanoic acid. Results showed this to be generally true, but sev‐eral minor component triglycerides not conform‐ing to this pattern were found.

The triglyceride composition ofMyrica carolinensis fruit coat fat (bayberry tallow)

“…Fatty acid compositions were determined by GLC of the methyl esters on a 6 ft by 0.125 in. column containing 20% (w/w) diethylene glycol succinate polyester coated on acid washed 60/80 mesh Chromosorb W as previously described (12). All fatty acid compositions are reported in mole percent to better show their relationship to triglyceride composition.…”

Gas‐liquid chromatography of triglycerides from erucic acid oils and fish oils

“…GLC is of greatest value when it is used as the final step in a series of consecutive fractionation techniques (8). ACKNO~rLEDG1VIENTS…”

“…(GLC) of triglyeerides containing C~ through C~ fatty acids is now used in many laboratories (1)(2)(3)(4)(5)(6)(7)(8)(9), erueic acid oils and fish oils have not been analyzed by this technique. Triglycerides containing long chain fatty acids usually show poor peak resolution and substantial losses during GLC.…”

“…prepared from the purified t riglycerides using the standard H.~SO4-methanolysis technique (8). The fatty acid composition was then determined by GLC analysis of the methyl esters on a 6 ft by 0.093 in.…”

“…I.D. column containing 20% butanediol sueeinate polyester coated on acid-washed Chromosorb W as previously described (8). Peaks were identified by comparison with the elution times of known compounds and by comparison with the compositions reported by other workers for the 216 same oils (]1-15).…”