Analysis of Transcripts Expressed in One-Day-Old Larvae and Fifth Instar Silk Glands of Tasar Silkworm,<i>Antheraea mylitta</i>

Maity, Samita; Goel, Sagar; Roy, Sudip; Ghorai, Suvankar; Bhattacharyya, Swati; Venugopalan, A V; Ghosh, Ananta K.

doi:10.1155/2010/246738

Cited by 7 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…mori and the Antheraea species. Maity et al searched for sericins by screening a silk gland-specific cDNA library from A. mylitta.…”

Section: Introductioncontrasting

confidence: 96%

“…The amino acid composition was different from that of B. mori sericins and the antibody recognized some antigens in the cocoon extract from Antheraea assamensis but not from B. mori, suggesting considerable differences between the sericins of B. mori and the Antheraea species. Maity et al 18 searched for sericins by screening a silk gland-specific cDNA library from A. mylitta. They obtained 2476 EST sequences that included Hf ibroin and seroin cDNAs but attempts to detect sericin-like genes failed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sericin Composition in the Silk of Antheraea yamamai

et al. 2016

View full text Add to dashboard Cite

The silks produced by caterpillars consist of fibroin proteins that form two core filaments, and sericin proteins that seal filaments into a fiber and conglutinate fibers in the cocoon. Sericin genes are well-known in Bombyx mori (Bombycidae) but have received little attention in other insects. This paper shows that Antheraea yamamai (Saturniidae) contains five sericin genes very different from the three sericin genes of B. mori. In spite of differences, all known sericins are characterized by short exons 1 and 2 (out of 3-12 exons), expression in the middle silk gland section, presence of repeats with high contents of Ser and charged amino acid residues, and secretion as a sticky silk component soluble in hot water. The B. mori sericins represent tentative phylogenetic lineages (I) BmSer1 and orthologs in Saturniidae, (II) BmSer2, and (III) BmSer3 and related sericins of Saturniidae and of the pyralid Galleria mellonella. The lineage (IV) seems to be limited to Saturniidae. Concerted evolution of the sericin genes was apparently associated with gene amplifications as well as gene loses. Differences in the silk fiber morphology indicate that the cocktail of sericins linking the filaments and coating the fiber is modified during spinning. Silks are composite biomaterials of conserved function in spite of great diversity of their composition.

show abstract

“…mori and the Antheraea species. Maity et al searched for sericins by screening a silk gland-specific cDNA library from A. mylitta.…”

Section: Introductioncontrasting

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Sericin Composition in the Silk of Antheraea yamamai

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Functional genomics has made it possible to begin investigating and exploiting genetic resources from the wild silkmoths and genetically modifying B. mori via gene mining or manipulation, with the promise of using domesticated species to produce silk with different properties usually associated with their wild counterparts [ 6 ]. However, to date, so far only low-coverage EST data are available for A. assama , S. cynthia and A. mylitta [ 7 , 8 ], and most studies on silk protein structure have only provided limited overviews [ 7 , 9 - 12 ]. The functional complexity of the silkmoth transcriptome for the Saturniidae silkmoths has not yet been sufficiently clarified.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analyses on silk glands of six silkmoths imply the genetic basis of silk structure and coloration

et al. 2015

View full text Add to dashboard Cite

BackgroundSilk has numerous unique properties that make it a staple of textile manufacturing for several thousand years. However, wider applications of silk in modern have been stalled due to limitations of traditional silk produced by Bombyx mori. While silk is commonly produced by B. mori, several wild non-mulberry silkmoths--especially members of family Saturniidae--produce silk with superior properties that may be useful for wider applications. Further utilization of such silks is hampered by the non-domestication status or limited culturing population of wild silkworms. To date there is insufficient basic genomic or transcriptomic data on these organisms or their silk production.ResultsWe sequenced and compared the transcriptomes of silk glands of six Saturniidae wild silkmoth species through next-generation sequencing technology, identifying 37758 ~ 51734 silkmoth unigenes, at least 36.3% of which are annotated with an e-value less than 10−5. Sequence analyses of these unigenes identified a batch of genes specific to Saturniidae that are enriched in growth and development. Analyses of silk proteins including fibroin and sericin indicate intra-genus conservation and inter-genus diversification of silk protein features among the wild silkmoths, e.g., isoelectric points, hydrophilicity profile and amino acid composition in motifs of silk H-fibroin. Interestingly, we identified p25 in two of the silkmoths, which were previously predicted to be absent in Saturniidae. There are rapid evolutionary changes in sericin proteins, which might account for the highly heterogeneity of sericin in Saturniidae silkmoths. Within the six sikmoths, both colored-cocoon silkmoth specific transcripts and differentially expressed genes between the colored-cocoon and non-colored-cocoon silkmoths are significantly enriched in catalytic activity, especially transferase activity, suggesting potentially viable targets for future gene mining or genetic manipulation.ConclusionsOur results characterize novel and potentially valuable gene resources of saturniid silkmoths that may facilitate future genetic improvement and modification of mulberry silkworms. Our results suggest that the disparate features of silk--coloration, retention, strength, etc. --are likely not only due to silk proteins, but also to the environment of silk assembly, and more specifically, that stable silk coloration exhibited by some Saturniidae silkmoths may be attributable to active catalytic progress in pigmentation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1420-9) contains supplementary material, which is available to authorized users.

show abstract

“…Compared to cellulose and other synthetic polymers, silk is peculiar for its durability, high strength, luster, and other unique features [1]. Among the Lepidopterans, the silk moths, especially Bombyx mori, Antheraea pernyi, Antheraea mylitta, and Antheraea assama play an important role in rural economy of many developing nations [2]. The importance of silk protein increased because of its potential use as a natural biopolymer for tissue engineering and biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

“…Few other wild silks are Mopani silk from South Africa, Saturniidae silk from Thailand, Assam silks (Muga, Eri, and Pat) from India, Tussah Silk from China, and Tassar Silk from India. Six million people in India alone are involved in sericulture, and one of the most economically important species for sericulture in India is the wild type nonmulberry silkworm, A. mylitta [2]. Tassar is a multivoltine type of silk and is mostly produced by tribal by rearing silkworms on forest plants (Terminalia arjuna, Terminalia tomentosas and Shorea robusta) [3].…”

Section: Introductionmentioning

confidence: 99%

Crystal Structure of Locally Available Tassar Fibers Based on Amino Acid Sequence: Using X-Ray Data and LALS Method

Parameswara

Somashekar

2011

ISRN Materials Science

View full text Add to dashboard Cite

X-ray diffraction pattern from locally available Tassar fibers was recorded using imaging plate system (Dip-100S). The pattern was used along with fiber processing software to identify the reflections and to compute X-ray intensities. A molecular model was first constructed with standard bond lengths and angles using helical symmetry and layer-line spacing observed in the X-ray pattern. The model was then refined against observed X-ray data using linked-atom least-squares (LALS) method. The crystal and molecular structure of Tassar fibers is reported. It is concluded that the sheet structures formed by hydrogen bonds assume the antipolar-antiparallel arrangement.

show abstract

Analysis of Transcripts Expressed in One-Day-Old Larvae and Fifth Instar Silk Glands of Tasar Silkworm,Antheraea mylitta

Cited by 7 publications

References 27 publications

Sericin Composition in the Silk of Antheraea yamamai

Sericin Composition in the Silk of Antheraea yamamai

Comparative transcriptome analyses on silk glands of six silkmoths imply the genetic basis of silk structure and coloration

Crystal Structure of Locally Available Tassar Fibers Based on Amino Acid Sequence: Using X-Ray Data and LALS Method

Contact Info

Product

Resources

About