Analysis of trace volatile metabolites in serum and plasma

Zlatkis, A.; Bertsch, W.; Bafus, Donald A.; Liebich, H.M.

doi:10.1016/s0021-9673(01)97916-6

Cited by 41 publications

(4 citation statements)

References 7 publications

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“…Papers presented at the 10th International Symposium on Chromatography held in Barcelona, Spain, September 30-October 4, 1974, are published in the October, November, and December, 1974, issues of the Journal of Chromatographic Science, and the proceedings of the Tenth International Symposium on Advances in Chromatography, 1975, held in Munich, Germany, November [3][4][5][6]1975, appear in the special October119751issue of the Journal of Chromatography. Reprints of this issue, edited by Zlatkis and Ettre (1741), are also available in book form. Proceedings of the third symposium of the Russian Advances in Gas Chromatography series held in Kazan, USSR, in 1973 are now available (1593) as is the collection of papers on new sorbents for GC presented in Leningrad in May 1972, commemorating the 100th anniversary of M. S. Tsevett's birthday (1199).…”

Section: Books and Reviewsmentioning

confidence: 99%

Gas chromatography

Cram¹,

Juvet²

1976

Anal. Chem.

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Section: Books and Reviewsmentioning

confidence: 99%

Gas chromatography

Cram¹,

Juvet²

1976

Anal. Chem.

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“…Extensive tables of normal reference ranges have been published for many blood gases, ions and about 100 metabolites [22], [24], [25], [26], [27]. In addition to these referential clinical chemistry studies, several groups have applied various “global” metabolomic or metabolite profiling methods, such as high resolution nuclear magnetic resonance (NMR) spectroscopy [28], [29], high performance liquid chromatography (HPLC) [30], amino acid analysis [31], [32] liquid chromatography – mass spectrometry (LC-MS) [33], [34], high performance liquid chromatography – mass spectrometry/mass spectrometry (HPLC-MS/MS) [35], gas chromatography – mass spectrometry (GC-MS) [36], [37], high resolution capillary GC-MS [38], GCxGC-MS [39], ultrahigh performance liquid chromatography – mass spectrometry (UPLC-MS) [40] and high resolution reversed-phase LC (RPLC) with high resolution quadrupole time-of-flight mass spectrometry (QqTOF) [41] to characterize the serum/plasma metabolome with varying degrees of success. Perhaps the most complete global characterization of the blood metabolome to date was described by Lawton and colleagues [13].…”

Section: Introductionmentioning

confidence: 99%

The Human Serum Metabolome

et al. 2011

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Continuing improvements in analytical technology along with an increased interest in performing comprehensive, quantitative metabolic profiling, is leading to increased interest pressures within the metabolomics community to develop centralized metabolite reference resources for certain clinically important biofluids, such as cerebrospinal fluid, urine and blood. As part of an ongoing effort to systematically characterize the human metabolome through the Human Metabolome Project, we have undertaken the task of characterizing the human serum metabolome. In doing so, we have combined targeted and non-targeted NMR, GC-MS and LC-MS methods with computer-aided literature mining to identify and quantify a comprehensive, if not absolutely complete, set of metabolites commonly detected and quantified (with today's technology) in the human serum metabolome. Our use of multiple metabolomics platforms and technologies allowed us to substantially enhance the level of metabolome coverage while critically assessing the relative strengths and weaknesses of these platforms or technologies. Tables containing the complete set of 4229 confirmed and highly probable human serum compounds, their concentrations, related literature references and links to their known disease associations are freely available at http://www.serummetabolome.ca.

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“…Volatile compounds found in human serum include many potential substrates or products of ADH reactions: ethanol/acetaldehyde, 1-propanol/1-propanal, 2-propanol/acetone, 1-butanol/1-butanal, 2-butanol/2-butanone, 2-butenal, 2-methylpropanol/2-methylpropanal, 1-pentanol/1-pentanal, 2-methylbutanol/2-methylbutanal, 3-methylbutanol/3-methylbutanal, trans -2-methyl-2-butenal, 2-pentanone, 3-pentanone, 3-penten-2-one, 1-hexanol/1-hexanal, 2-hexanol/2-hexanone, 4-methyl-2-pentanone, 2-heptanone, 3-heptanone, 4-heptanone, octanal, 2-octanol/2-octanone, 4-octanone, 6-methyl-2-heptanone, 5-methyl-3-heptanone, nonanal, 2-nonenal, n -nona-2,4-dienal, 5-nonanone, 1-decanal, 1-undecanal, furfural, cyclohexanone, benzaldehyde, and 6-methyl-5-acetophenone [63-68]. Which substrates are metabolized by ADHs is thus of interest.…”

Section: Discussionmentioning

confidence: 99%

Contribution of liver alcohol dehydrogenase to metabolism of alcohols in rats

Plapp

Leidal

Murch

et al. 2015

Chemico-Biological Interactions

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The kinetics of oxidation of various alcohols by purified rat liver alcohol dehydrogenase (ADH) were compared with the kinetics of elimination of the alcohols in rats in order to investigate the roles of ADH and other factors that contribute to the rates of metabolism of alcohols. Primary alcohols (ethanol, 1-propanol, 1-butanol, 2-methyl-1-propanol, 3-methyl-1-butanol) and diols (1,3-propanediol, 1,3-butanediol, 1,4-butanediol, 1,5-pentanediol) were eliminated in rats with zero-order kinetics at doses of 5–20 mmole/kg. Ethanol was eliminated most rapidly, at 7.9 mmole/kg•h. Secondary alcohols (2-propanol-d7, 2-propanol, 2-butanol, 3-pentanol, cyclopentanol, cyclohexanol) were eliminated with first order kinetics at doses of 5–10 mmole/kg, and the corresponding ketones were formed and slowly eliminated with zero or first order kinetics. The rates of elimination of various alcohols were inhibited on average 73% (55% for 2-propanol to 90% for ethanol) by 1 mmole/kg of 4-methylpyrazole, a good inhibitor of ADH, indicating a major role for ADH in the metabolism of the alcohols. The Michaelis kinetic constants from in vitro studies (pH 7.3, 37 °C) with isolated rat liver enzyme were used to calculate the expected relative rates of metabolism in rats. The rates of elimination generally increased with increased activity of ADH, but a maximum rate of 6 ± 1 mmole/kg•h was observed for the best substrates, suggesting that ADH activity is not solely rate-limiting. Because secondary alcohols only require one NAD+ for the conversion to ketones whereas primary alcohols require two equivalents of NAD+ for oxidation to the carboxylic acids, it appears that the rate of oxidation of NADH to NAD+ is not a major limiting factor for metabolism of these alcohols, but the rate-limiting factors are yet to be identified.

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Analysis of trace volatile metabolites in serum and plasma

Cited by 41 publications

References 7 publications

Gas chromatography

Gas chromatography

The Human Serum Metabolome

Contribution of liver alcohol dehydrogenase to metabolism of alcohols in rats

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