Analysis of trace elements distributed in blood

Stiefel, Th.; Schulze, K.; Tölg, G.; Zorn, H.

doi:10.1007/bf00488332

Cited by 16 publications

(12 citation statements)

References 3 publications

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“…Especially gel electrophoresis is one of the most important electrophoretic methods for isolating all kinds of proteins, peptides and enzymes in biological samples. In the past, the analytical criterions required for isolating biologically-active compounds, mentioned above, could not be accomplished thoroughly by some of these procedures [29][30][31][32]. By a new approach concerning a preparative native polyacrylamide gel electrophoresis (PAGE) method for isolating native metalloproteins in biological fluids, it is concluded that the above mentioned criterions apply to this method.…”

Section: Analytical Prerequisitesmentioning

confidence: 99%

“…The former state-of-the-art of a preparative native continuous polyacrylamide gel electrophoresis (PAGE) is exemplary shown in two studies dealing, for example, with the separation of the native high molecular weight beryllium-proteins of human serum under physiological conditions [29]. In the first case the chemical stability of the detected Be-proteins after the PAGE trials was not verified by an independent method, e.g.…”

Section: Quantitative Preparative Native Conti-nuous Page (Qpnc-page)mentioning

confidence: 99%

“…by using NMR spectroscopy as detection method. Therefore, preparative analytical systems with continuous elution of the separated compounds [29,30] should be applied. Furthermore, as a major prerequisite the chemical stability of the native proteins during the purification and separation processes of a complex biological sample has to be checked by an independent method [31].…”

Section: Analytical Prerequisitesmentioning

confidence: 99%

“…Gel electrophoresis is well-known as a semi-quantitative procedure so far [29][30][31][32]. The former state-of-the-art of a preparative native continuous polyacrylamide gel electrophoresis (PAGE) is exemplary shown in two studies dealing, for example, with the separation of the native high molecular weight beryllium-proteins of human serum under physiological conditions [29].…”

Section: Quantitative Preparative Native Conti-nuous Page (Qpnc-page)mentioning

confidence: 99%

“…In this context some cryocontainers for the native storing and transport of different biological samples may also be an important prerequisite [26][27][28]. Furthermore, the applied analytical systems must be suitable for separating preparative amounts (mg-range) of complex protein samples because the physiological concentrations of the different metalloproteins in various biofluids may be very small [29,30].…”

Section: Analytical Prerequisitesmentioning

confidence: 99%

See 4 more Smart Citations

Important Contributions of a New Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) Procedure for Elucidating Metal Cofactor Metabolisms in Protein-Misfolding Diseases - A Theory

Kastenholz¹

2006

PPL

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The quantitative analysis of metallochaperone proteins in biofluids (e.g. blood, liquor) may be a major prerequisite for clinical investigations concerning the structure-function relationships of biologically-active metal cofactor-containing chaperones in protein-misfolding diseases (e.g. Alzheimer's or related diseases). For these purposes, a new state-of-the-art gel electrophoresis [quantitative preparative native continuous polyacrylamide gel electrophoresis procedure (QPNC-PAGE)] combined with biological mass and NMR spectrometries might essentially contribute to provide fundamental insights into the metabolisms of important metal cofactors in biological systems and the proper folding of metallochaperones in conformational diseases.

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Section: Analytical Prerequisitesmentioning

confidence: 99%

Section: Quantitative Preparative Native Conti-nuous Page (Qpnc-page)mentioning

confidence: 99%

Section: Analytical Prerequisitesmentioning

confidence: 99%

Section: Quantitative Preparative Native Conti-nuous Page (Qpnc-page)mentioning

confidence: 99%

Section: Analytical Prerequisitesmentioning

confidence: 99%

See 3 more Smart Citations

Important Contributions of a New Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) Procedure for Elucidating Metal Cofactor Metabolisms in Protein-Misfolding Diseases - A Theory

Kastenholz¹

2006

PPL

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Elementspeziesanalytik (ESA)

1995

Kopplungstechniken Zur Elementspeziesanalytik

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Great Fear of Small Amounts of Elements—The Significance of Analytical Chemistry in our Modern Industrialized Community as Exemplified by Trace Element Analysis

Tölg

Garten

1985

Angew. Chem. Int. Ed. Engl.

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Analytical chemistry is consolidating an important position within the framework of our modern industrial community; the frontiers of trace (and ultra-trace) analysis have expanded into new territories, thus demanding a constant change in our mode of thinking in a substance-related manner in analytical chemistry. An outline of the development of analytical chemistry during this century reveals a period of underdeveloped research and education followed by a current phase of impetuous advancement. However, as a result of increasingly antagonistic sectional convictions in the public mind concerning reservations against, as well as efforts towards, efficient technological progress, this advancement evokes new existential risks for analytical chemistry-viz. either to be used in an uncritical way or to fall into discredit following slogans like 'high-performance analytical chemistry is to blame for it all!' A much more constructive consideration says that risks can be estimated and evaluated solely by means of a highly efficient analytical chemistry, when used with a sense of responsibility. Analysts may help to clarify and to cope with the increasing fear of decreasingly smaller amounts of trace elements-in both adverse groups in our community. Strategies necessary to gain this end are outlined with regard to a methodological as well as a political platform. Angew Chem. In/. Ed. Enyl. 24 (1985) 485-4414 0 VCH Vrrlagsgesell.rchaJt mbH. 0-6940 Weinheini. 1985 0570-0833/85/0606-0485 $ 02.50/0 Angew. Chem. Int. Ed. Engl. 24 /19HS) 485-494 Anqew. Chem. Inr. Ed. Engl. 24 (1985) 485-494 4x7

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Analysis of trace elements distributed in blood

Cited by 16 publications

References 3 publications

Important Contributions of a New Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) Procedure for Elucidating Metal Cofactor Metabolisms in Protein-Misfolding Diseases - A Theory

Important Contributions of a New Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) Procedure for Elucidating Metal Cofactor Metabolisms in Protein-Misfolding Diseases - A Theory

Elementspeziesanalytik (ESA)

Great Fear of Small Amounts of Elements—The Significance of Analytical Chemistry in our Modern Industrialized Community as Exemplified by Trace Element Analysis

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