Analysis of trace element content in hair of autistic children

Fiłon, Joanna; J., Jolanta Ustymowicz-Farbiszewska; Karczewski, Jakub; Żendzian-Piotrowska, Małgorzata

doi:10.5601/jelem.2016.21.4.1355

Cited by 4 publications

(3 citation statements)

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“…The minimal Ca concentration in this group amounted to approximately 70 ppm [16,17]. Apart from Ca deficiency, many children with autism also had a deficiency of other elements, including Zn, Mg, Mn or Cu [12,16,17,19,49]. It was also demonstrated that children with autism had significantly lower Ca content in the hair (by 20%) compared to normal children [27].…”

Section: Discussionmentioning

confidence: 89%

Analysis of lead, arsenic and calcium content in the hair of children with autism spectrum disorder

Fiłon

Ustymowicz-Farbiszewska

Krajewska-Kułak

2020

BMC Public Health

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Background: Explanation of the pathogenesis and treatment of autism spectrum disorders (ASD) is one of the most significant challenges for scientists today. It is believed that a major pathogenetic factor of this condition is epigenetic changes caused by environmental factors, including toxic metals (cadmium (Cd), lead (Pb), mercury (Hg), aluminium (Al), and arsenic (As)). The nervous system may also be affected by deficiencies of both micro-and macroelements (e.g. calcium (Ca), zinc (Zn)). The aim of the study was to analyze the concentrations of Pb, As, and Ca in the hair of children with ASD and a control group. Methods: The materials for the study comprised hair samples collected from 30 children diagnosed with ASD (case group) and 30 children randomly selected from the general population of Bialystok and surrounding region (control group). Concentrations of Pb, As, and Ca were tested with electron microscopy scanning method. Next, the content of the analyzed elements in the hair was assessed as well as their impact on autism development in the children and the mutual interactions between them. The obtained results were statistically analyzed with Statistica PL 12.5., using the Mann-Whitney U test, and Spearman correlation coefficient. Results: Mean Ca level in the hair of the case group was lower than the mean level of this element in the control group. Mean As and Pb concentration in the hair of children with ASD was statistically significantly higher than the mean concentration of this element in the hair of children without neurological disorders. Statistically insignificant weak positive correlations between Ca and As content and negative between Ca and Pb in the hair of children from the case group were noted. Also, statistically significant mean positive correlations between Pb and As were observed. Conclusions: In this small study, according to the observations, children diagnosed with ASD suffer from Ca deficiency and toxic metal overload (As and Pb). These abnormalities may play the main role, as an environmental factor, in the pathogenesis of the analyzed disorder.

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Section: Discussionmentioning

confidence: 89%

Analysis of lead, arsenic and calcium content in the hair of children with autism spectrum disorder

Fiłon

Ustymowicz-Farbiszewska

Krajewska-Kułak

2020

BMC Public Health

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“…Optimal parameters for both techniques are presented in Table 6. Integration times for monitored isotopes 5 ms: 56 Fe, 10 ms: 24 Mg, 27 Al 50 ms: 31 P, 39 K, 44 Ca, 45 Sc 100 ms: 7 Li, 9 Be, 47 Ti, 51 V, 52 Cr, 55 Mn, 59 Co, 60 Ni, 63 Cu, 66 Zn, 71 Ga, 72 Ge IS , 88 Sr, 89 Y IS , 95 Mo, 103 Rh IS , 107 Ag, 115 In, 118 Sn, 121 Sb, 125 Te, 127 I, 137 Ba, 201 Hg, 205 Tl, 206 Pb, 209 Bi, 232 Th, 238 U 300 ms: 75 As, 78 Se, 79 Br, 23 Na 1000 ms: 111 Cd *, **, ***-signals selected to monitor elements related to X-ray emitted when replenishing the K and L shells with electron-K α , K β , L α , respectively.…”

Section: Edx and Icp Ms/ms Detection Conditionsmentioning

confidence: 99%

“…It is, therefore, not surprising that different studies have shown a positive association between metal levels in patients with diseases, such as Alzheimer's disease, breast cancer, endocrine disorders, hypertension, rheumatoid arthritis, and childhood neurocognitive disorders [2,5,6]. For example, bioaccumulation of lead, cadmium, cobalt, chromium, and copper causes damage to the nervous system, kidneys, liver, and reproductive system [2,7,8]. Therefore, there is an increasing demand for cheap and easily accessible methods to control the level of exposure of workers in industry and diagnostic methods enabling the prevention and monitoring of the patient's condition during therapy.…”

Section: Introductionmentioning

confidence: 99%

Multi-Elemental Analysis of Hair and Fingernails Using Energy-Dispersive X-ray Fluorescence (ED XRF) Method Supported by Inductively Coupled Plasma Mass Spectrometry (ICP MS)

Mierzyńska,

Niemirska,

Zgonina

et al. 2024

Molecules

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This work compared the multi-element analysis of human hair and nails using inductively coupled plasma mass spectrometry (ICP MS) with an easy, fast, cheap, non-destructive method using energy-dispersive x-ray fluorescence (ED XRF). The ICP MS-based method was more sensitive (over 30 elements could be quantified) and costly (requiring more time, samples, and chemicals). The EDX-based method required laboratory and certified reference materials made of hair for instrument calibration. It was less sensitive (16 elements could be quantified: S, Si, Ca, Br, Fe, Cu, Cr, Mg, Si, K, Mn, Ni, Zn, Se, Sr, Pb), but it allowed us to replace troublesome grinding with the dissolution of keratin-based material with an alkalic agent (tetramethylammonium hydroxide, TMAH) and the formation of stable-for-days pellets. This method is simple, enables automation, and, due to the modification of wells in the autosampler of the EDX system via the immersion of home-designed inserts, it requires smaller amounts of biological material and binder (down to 70 mg instead of 500 mg required by commercially available instrument) to perform analysis. It was concluded that the EDX-based method offers complementary selectivity and sensitivity to ICP MS with the possibility of sample reuse for further analysis.

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Effect of Casein Gluten Free Snack, Nutrition Education and Level of Pb and Zn Metal on Clinical Manifestation of Autism

Siahaan¹,

Lestrina²,

Tarigan³

et al. 2018

Asian J. of Clinical Nutrition

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Analysis of trace element content in hair of autistic children

Cited by 4 publications

References 0 publications

Analysis of lead, arsenic and calcium content in the hair of children with autism spectrum disorder

Analysis of lead, arsenic and calcium content in the hair of children with autism spectrum disorder

Multi-Elemental Analysis of Hair and Fingernails Using Energy-Dispersive X-ray Fluorescence (ED XRF) Method Supported by Inductively Coupled Plasma Mass Spectrometry (ICP MS)

Effect of Casein Gluten Free Snack, Nutrition Education and Level of Pb and Zn Metal on Clinical Manifestation of Autism

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