Analysis of Tissue Bioimpedance as a Measurement of Liver Steatosis: Experimental Model in Large Animals

Gonzalo, María Azucena; Martínez‐Beamonte, Roberto; Palacios, Pilar Tejada; Marín, Juan Ignacio; Castiella, Tomás; Surra, Joaquín C.; Burdı́o, Fernando; Sousa, Ramón; Güemes, Antonio; Osada, Jesús; García‐Gil, Alejandro

doi:10.1016/j.transproceed.2012.05.006

Cited by 4 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the main problems working with commercial crossbred swine is their genetic resistance to generate NAFLD due to the human directed selection over the last few decades to convert all their intakes into muscle weight gain 35 . Based on our previous experience with medium term experiments 36 , swine fed a high cholate, cholesterol, fructose and saturated fat and methionine- and choline-deficient diet (Table S1 ) for two months could overcome this drawback for the use of this large animal as a NAFLD model. In fact, the percentage of hepatic area occupied by lipid droplets (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The availability of a large animal model of NAFLD with initial NASH in a short period may be of particular interest to understand the onset of NAFLD and its progression to NASH, to explore new treatments for this ailment 41 and to better characterize the steatotic liver for transplant procedures 36 . In these two experiments, 20 animals were provided the steatotic diet and all of them reached NAFLD according FLIP algorithm.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model

Herrera-Marcos

Martínez‐Beamonte

Macías-Herranz

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Non-alcoholic fatty liver disease (NAFLD) is currently a growing epidemic disease that can lead to cirrhosis and hepatic cancer when it evolves into non-alcoholic steatohepatitis (NASH), a gap not well understood. To characterize this disease, pigs, considered to be one of the most similar to human experimental animal models, were used. To date, all swine-based settings have been carried out using rare predisposed breeds or long-term experiments. Herein, we fully describe a new experimental swine model for initial and reversible NASH using cross-bred animals fed on a high saturated fat, fructose, cholesterol, cholate, choline and methionine-deficient diet. To gain insight into the hepatic transcriptome that undergoes steatosis and steatohepatitis, we used RNA sequencing. This process significantly up-regulated 976 and down-regulated 209 genes mainly involved in cellular processes. Gene expression changes of 22 selected transcripts were verified by RT-qPCR. Lipid droplet area was positively associated with CD68, GPNMB, LGALS3, SLC51B and SPP1, and negatively with SQLE expressions. When these genes were tested in a second experiment of NASH reversion, LGALS3, SLC51B and SPP1 significantly decreased their expression. However, only LGALS3 was associated with lipid droplet areas. Our results suggest a role for LGALS3 in the transition of NAFLD to NASH.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model

Herrera-Marcos

Martínez‐Beamonte

Macías-Herranz

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…We believe that one of the greatest strengths of our study was the experimental model used. It is original from our group who are experienced in another work [45]. In addition, we tried to individualize the effect of melatonin from the effect of the intake of the steatotic diet.…”

Section: Pre Postmentioning

confidence: 99%

Effect of Melatonin as an Antioxidant Drug to Reverse Hepatic Steatosis: Experimental Model

Soriano

Güemes

Pola³

et al. 2020

Canadian Journal of Gastroenterology and Hepatology

View full text Add to dashboard Cite

Introduction. The hepatic steatosis of the nonalcoholic origin or NAFLD is increasing at present, particularly in Western countries, parallel to the increase in obesity, constituting one of the most prevalent hepatic processes in the Western society. Melatonin has been successfully tested in experimental models in mice as a drug capable of reversing steatosis. The effect of melatonin on fat metabolism can be summarized as a decrease in lipid peroxidation and a decrease in oxidative stress, biochemical phenomena intimately related to fat deposition in the hepatocyte. There are hardly any studies in large animals. Objective. In this study, we investigate the effects of melatonin administered orally at a dose of 10 mg/kg/day to reverse established hepatic steatosis induced by a special diet in a porcine animal model. Materials and Methods. We analyze the parameters of oxidative stress: malondialdehyde (MDA), 4-hydroxyalkenals (4-HDA), and carbonyls, degree of fat infiltration (analyzed by direct vision by a pathologist and by means of a computer program of image treatment), and serological parameters of lipid metabolism and hepatic damage. These parameters were analyzed in animals to which hepatic steatosis was induced by means of dietary modifications. Results. We have not been able to demonstrate globally a beneficial effect of melatonin in the improvement or reversal of liver steatosis once established, induced by diet in a porcine animal model. However, we have found several signs of improvement at the histological level, at the level of lipid metabolism, and at the level of oxidative stress parameters. We have verified in our study that, in the histological analysis of the liver sample by means of the program image treatment (free of subjectivity) of the animals that continue with the diet, those that consume melatonin do not increase steatosis as much as those that do not consume it significantly (p=0.002). Regarding the parameters of oxidative stress, MDA modifies in a significant manner within the group of animals that continue with the diet and take melatonin (p=0.004). As for lipid metabolism, animals that maintain the steatotic diet and take melatonin lower total and LDL cholesterol levels and increase HDL levels, although these results do not acquire statistical significance. Conclusions. In this study, it has not been possible to demonstrate a beneficial effect of melatonin in the improvement or reversal of liver steatosis once established and induced by diet in the porcine model. It is true that signs of improvement have been found at the histological level, at the level of lipid metabolism, and at the level of oxidative stress phenomena, when comparing animals with established steatosis that are treated with melatonin with those who do not take it. This work is the first study conducted in a large animal model in which the effect of melatonin is studied as a treatment in the reversal of established hepatic steatosis.

show abstract

“…Non-invasively and easily measuring the degree of fatty liver may help in organ retrieval by allowing repeated measurements and determining the heterogeneous fat distribution in the liver. Previous studies using animal models have reported good correlations between BI measurements and liver steatosis (9)(10)(11). Here, we evaluated whether BI could be used to evaluate the rate of liver steatosis in human livers in a pilot study of non-cirrhotic patients who underwent a hepatectomy.…”

mentioning

confidence: 99%

A Simple Rapid Method for Measuring Liver Steatosis Using Bioelectrical Impedance

Yoshimoto-Haramura

Hara

Soyama

et al. 2022

In Vivo

View full text Add to dashboard Cite

Background/Aim: Easy measurement of liver steatosis without pathological diagnosis may help improve donor surgery efficiency and increase the chances of organ donations. We analyzed the correlations between bioelectrical impedance (BI) in human livers, liver fat content, and pathological findings. Materials and Methods: Sixteen tumor-free liver specimens resected during elective oncological surgery were analyzed. All samples were stored in ice chilled saline before BI measurement. The BI measurement was performed using a device with the tetrapolar circuit method in which the current and voltage electrodes are independent. Liver cholesterol and triglyceride levels were investigated from the same specimen using the Soxhlet extraction method. Pathological findings were examined by counting the number of hepatocytes with fatty changes per high-power field. Results: The median liver steatosis percentage was 0.4%. The liver steatosis percentage was significantly correlated with the intrahepatic triglyceride content (r=0.82, p<0.001). Linear regression of the measurements and predicted values yielded an r 2 of 0.63 between the BI at 100 kHz and liver steatosis, indicating reasonable agreement (p<0.001). Conclusion: BI analysis is a simple, non-invasive method that can be easily applied to evaluate liver steatosis.

show abstract

Analysis of Tissue Bioimpedance as a Measurement of Liver Steatosis: Experimental Model in Large Animals

Cited by 4 publications

References 13 publications

Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model

Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model

Effect of Melatonin as an Antioxidant Drug to Reverse Hepatic Steatosis: Experimental Model

A Simple Rapid Method for Measuring Liver Steatosis Using Bioelectrical Impedance

Contact Info

Product

Resources

About