Analysis of three whole-arm translocations in a mouse sarcoma cell line

Abstract

Three apparently whole-arm translocation chromosomes in the mouse sarcoma 180 ascites cell line have been studied by G- and C-banding and by Hoechst staining. All three chromosomes appear to have two centromeres. Both centromeres of one, which are very close together, are likely to be active and to produce parallel separation of the chromatids. The centromeres of the other two chromosomes are well separated. One of these two centromeres may be inactive either because the kinetochore organizer has been inactivated or because the kinetochore plate has been deleted, leaving the AT-rich centromeric constituative heterochromatin intact. The possibility that these whole arm translocations arose by telomeric fusion and the molecular basis of such fusions are discussed.

Chromosomal disruption and rearrangements during murine sarcoma development converge to stable karyotypic formation kept by telomerase overexpression

“…The structural alterations observed in TG180 were also observed in haematological cancers [ 42 , 43 ], solid tumors [ 44 ], at the onset of acute myelogenousleukemia [ 45 ], and in another murine sarcoma cell line [ 46 ]. Many chromosome translocations of tumorshave been studied and their gene fusion products identified [ 47 ].…”

Adhesion of Pathogenic Bacteria to Polystyrene, Skin and Gut Mucus of Gilthead Seabream, Infectious Capacity and Antibiotics Susceptibility

“…SAF-1 was used because it's good adaptation for growth in a standard medium without special requirements. Besides, the adaptation of cell lines to in vitro conditions is often associated with dynamic chromosomal changes affecting chromosome number and/or morphology, giving rise in some cases to marker chromosomes which are line-specific (Ghosh and Chaudhuri, 1984). In the SAF-1 line neither chromosome changes nor differences between passages have been detected, suggesting that besides having a normal karyotype, this line is rather stable.…”