Analysis of thiamine and its phosphate esters by high-performamze liquid chromatography

Ishii, Kazuhiko; Sarai, Keisuke; Sanemori, Hiroshi; Kawasaki, Takashi

doi:10.1016/0003-2697(79)90345-2

Cited by 65 publications

(13 citation statements)

References 3 publications

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“…Several methods for the determination of thiamin and its phosphate esters were described in previous publications and different extraction and derivatization methods were used [17][18][19][20]. While other published methods focused on improvements in chromatography and time of analysis we are now proposing improvements which offer a convenient and fast sample extraction procedure and a small sample volume of 100 l. In contrast, existing methods require from 500 l [11,25] to 1000 l [26] of whole blood.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of thiamin diphosphate in whole blood samples by high-performance liquid chromatography—A method suitable for pediatric diagnostics

Körner¹,

Vierzig²,

Roth³

et al. 2009

Journal of Chromatography B

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Section: Discussionmentioning

confidence: 99%

“…The buffer's pH was 8.2 since the fluorescence of TDP reaches a maximum above pH 8 [20]. The mobile phase was delivered at a flow rate of 1.0 ml/min.…”

Section: Chromatographic Conditions and Detectionmentioning

confidence: 99%

Determination of thiamin diphosphate in whole blood samples by high-performance liquid chromatography—A method suitable for pediatric diagnostics

Körner¹,

Vierzig²,

Roth³

et al. 2009

Journal of Chromatography B

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“…Specimens were centrifuged and plasma was separated and frozen. Plasma thiamine levels were measured by high-pressure liquid chromatography 9 performed at a commercial laboratory affiliate (Smith Kline Beecham French-Department of Pharmacology, Hahnemann University Hospital). Thiamine deficiency was defined as < 10 g/dL.…”

Section: Methodsmentioning

confidence: 99%

Low Plasma Thiamine Levels in Elder Patients Admitted through the Emergency Department

Lee

Chu

Satz

et al. 2000

Academic Emergency Medicine

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Abstract. Objectives:To determine the prevalence of thiamine deficiency in a high-risk group of elder emergency department (ED) patients who reside in nursing homes and need admission to the hospital, and to determine the effect of patients' diets on this prevalence. Methods: This was an observational pilot study of 75 consecutive ED patients aged 65 years or older who lived in a nursing home and were admitted to the hospital. Plasma thiamine levels were measured by high-pressure liquid chromatography on serum samples collected within 24 hours of hospital admission. Nursing home records were reviewed to determine whether patients received nutritional supplementation or enteral tube feedings. Results: Seventy patients participated and had a mean plasma thiamine level of 27.3 /dL (95% CI = 20.2 to 34.4). Fourteen percent (n = 10, 95% CI = 8% to 24%) were thiamine-deficient (<10 g/dL). Patients not receiving dietary supplements or tube feedings (n = 26) had lower mean thiamine levels (20.3 g/dL, 95% CI = 12.7 to 27.9) and were thiamine-deficient more often (27%) than patients receiving dietary support (n = 44, 31.5 g/dL, 95% CI = 24.7 to 38.3, 7% thiamine-deficient). Conclusions: Elder nursing home patients seen in the ED and admitted to the hospital are frequently thiamine-deficient. Empiric thiamine supplementation is often used in the ED for other high-risk patients, such as alcoholic individuals, and may be appropriate for high-risk elder patients. Further research is needed to determine whether thiamine supplementation in these patients can improve their clinical outcomes.

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“…Fluorescence of the thiochromes increases with increasing pH, reaching a maximum above pH 9 [18]. The determination of thiamin at such relatively high pH values offers some distinct advantages.…”

Section: Introductionmentioning

confidence: 99%

The analysis of thiamin and its derivatives in whole blood samples under high pH conditions of the mobile phase

Landeghem

Puts

Claessens

2005

Journal of Chromatography B

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Analysis of thiamine and its phosphate esters by high-performamze liquid chromatography

Cited by 65 publications

References 3 publications

Determination of thiamin diphosphate in whole blood samples by high-performance liquid chromatography—A method suitable for pediatric diagnostics

Determination of thiamin diphosphate in whole blood samples by high-performance liquid chromatography—A method suitable for pediatric diagnostics

Low Plasma Thiamine Levels in Elder Patients Admitted through the Emergency Department

The analysis of thiamin and its derivatives in whole blood samples under high pH conditions of the mobile phase

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