Analysis of the WUSCHEL-RELATED HOMEOBOX gene family in Pinus pinaster : New insights into the gene family evolution

Álvarez, José Marcos Alonso; Bueno, Natália Pieretti; Cañas, Rafael A.; Ávila, Concepción; Cánovas, Francisco M.; Ordás, Ricardo J.

doi:10.1016/j.plaphy.2017.12.031

Cited by 45 publications

(55 citation statements)

References 72 publications

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“…In contrast, WOX2 expression was detected in both embryogenic and non-embryogenic lines in P. radiata , although transcript levels were lower in the latter (Bravo et al, 2017). Maritime pine WOX gene family has been studied recently (Alvarez et al, 2018) and WOX2 expression has been shown to be highest at early embryogenic stages and decreased along the maturation process being lower in mature somatic embryos which agree with our WOX2 expression profile sampled at 0, 7, and 12 weeks in SK lines. Similar results were obtained in other conifers as Pinus sylvestris (Merino et al, 2018), Picea (Palovaara and Hakman, 2008; Klimaszewska et al, 2011), Larix decidua (Rupps et al, 2016), Pinus contorta (Park et al, 2010), and Chinese fir (Zhou et al, 2017).…”

Section: Discussionsupporting

confidence: 89%

“…However, studies on some of the genes associated with conifer embryogenesis as LEAFY COTYLEDON ( LEC1 ), WUSCHEL-related HOMEOBOX2 ( WOX2 ) and SOMATIC EMBRYOGENESIS RECEPTOR-like KINASE ( SERK ) demonstrated that the expression of these genes varied along embryogenesis and are differentially expressed in embryogenic and non-embryogenic cultures in some species (Yang and Zhang, 2010; Klimaszewska et al, 2016). Recently, Alvarez et al (2018) reported on the characterization of the WUSCHEL-RELATED HOMEOBOX gene family in maritime pine and its expression during SE.…”

Section: Introductionmentioning

confidence: 99%

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New Approaches to Optimize Somatic Embryogenesis in Maritime Pine

et al. 2019

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Maritime pine ( Pinus pinaster Aiton) is a coniferous native of the Mediterranean basin. Because of its adaptability to a wide range of environmental conditions, the species have become a model for studies in coniferous forest management and functional genomics. Somatic embryogenesis (SE) has been so far, the preferred biotechnological strategy for maritime pine breeding programs initiated at the middle-end of the 20th century. To overcome the limitations of the induction and maturation phases in maritime pine SE, we analyzed the possible maternal influence on the embryogenic capability of megagametophytes from controlled crosses, as well as the effect of the temperature and water availability during SE process on the production of plants. A strong maternal effect on the embryogenic potential of maritime pine megagametophytes was observed in our experiments using half-sib and full-sib progenies, while paternal effect was almost undetectable. Besides, it seems possible to improve somatic embryo production of maritime pine megagametophytes by adjusting optimal temperature throughout the process: 28°C during induction and proliferation, and 23°C during the maturation phase. Using induction and proliferation media with reduced water availability (6 g/L Gelrite) can also increase embryo production. Since other limitation of maritime pine SE is culture decline of embryogenic masses (EMs), that reduces embryo yield and germination, we assessed the profile of ABA and IAA and the expression of two embryogenesis-related genes ( LEC1 and WOX2 ) during maturation of EMs of two morphotypes that differed in their maturation capability. Spiky morphotype (SK), with high maturation capability, had a steady increase in both hormones along the 12 weeks of the maturation, whereas ABA content in smooth morphotype picked at the 4th week and dropped. EMs with this morphotype also had a higher IAA content at the beginning of the maturation. A decrease of LEC1 and WOX2 gene expression over the course of embryo development was found to be characteristic of the SK with high maturation capability.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

New Approaches to Optimize Somatic Embryogenesis in Maritime Pine

et al. 2019

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“…In addition, PpWOX5, which is orthologous to AtWOX5, shows maximum expression at the mature-embryo stage, with transcripts preferentially located to the root tip of seedlings. This, in combination with low expression levels in other seedling tissues, mirrors the expression pattern of AtWOX5, which has been shown to influence stem-cell function in Arabidopsis RAM (Alvarez et al 2017).…”

Section: Embryonic Wox Expression In Coniferssupporting

confidence: 53%

“…Alvarez et al () recently reported high expression of two Pinus pinaster WOX genes, both of which are closely related to AtWOX9 , in early and late embryos, with expression decreasing dramatically in germinated embryos. In addition, PpWOX5 , which is orthologous to AtWOX5 , shows maximum expression at the mature‐embryo stage, with transcripts preferentially located to the root tip of seedlings.…”

Section: Embryonic Wox Expression In Conifersmentioning

confidence: 99%

Control of root meristem establishment in conifers

Brunoni

Ljung

Bellini

2018

Physiologia Plantarum

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The evolution of terrestrial plant life was made possible by the establishment of a root system, which enabled plants to migrate from aquatic to terrestrial habitats. During evolution, root organization has gradually progressed from a very simple to a highly hierarchical architecture. Roots are initiated during embryogenesis and branch afterward through lateral root formation. Additionally, adventitious roots can be formed post-embryonically from aerial organs. Induction of adventitious roots (ARs) forms the basis of the vegetative propagation via cuttings in horticulture, agriculture and forestry. This method, together with somatic embryogenesis, is routinely used to clonally multiply conifers. In addition to being utilized as propagation techniques, adventitious rooting and somatic embryogenesis have emerged as versatile models to study cellular and molecular mechanisms of embryo formation and organogenesis of coniferous species. Both formation of the embryonic root and the AR primordia require the establishment of auxin gradients within cells that coordinate the developmental response. These processes also share key elements of the genetic regulatory networks that, e.g. are triggering cell fate. This minireview gives an overview of the molecular control mechanisms associated with root development in conifers, from initiation in the embryo to post-embryonic formation in cuttings.

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“…Among these CYCs, A-and B-type CYCs regulate the cell cycle progression through the S-, G2-and early M-phase, while D-type CYCs regulate the cell cycle progression of G1/S phase transition (Nafati et al 2010). The plantspecific homeobox transcription factor WUSCHELrelated homeobox (WOX) gene family plays vital roles in modulating plant growth and development by controlling stem cell activity in shoot/root apical meristem and cambium (Alvarez et al 2018, Li et al 2019.…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet-B radiation induces cell death in root tips and reprograms metabolism in Arabidopsis

Zhang¹,

Wang²,

Wang³

et al. 2020

Biol. plant.

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Ultraviolet-B (UV-B) radiation inhibits root system growth, however, the influence of UV-B radiation on the regulation of root development remains unclear. Here, we investigated the effects of UV-B radiation on root growth and metabolism in Arabidopsis using physiological, pharmacological, and transcriptome approaches. Our previous study has demonstrated that UV-B radiation depresses auxin accumulation in roots by reducing auxin biosynthesis, transport, and response. In this study, we found that UV-B radiation inhibited primary root (PR) growth by inducing root tip cell death and thereby disrupting cell division and elongation in root tips. The destructed root structure and distorted auxin flow caused by UV-B-induced root tip cell death also led to a reduced auxin accumulation in roots. Supplementation with an auxin α-naphthylacetic acid alleviated UV-B-repressed PR growth and further supported a notion that auxin is involved in UV-Brepressed PR growth. The UV-B radiation downregulated the expression of genes encoding the enzymes or regulators of the biosyntheses and degradations of the structural constituents of cell wall and genes involved in wax, cutin, and suberin biosyntheses, thereby repressing root system growth and development. The UV-B radiation also markedly repressed photosynthesis-related gene expression in roots, a non-photosynthetic organ. Taken together, this study suggests that UV-B radiation affects root growth by inducing cell death in root tips and reprogramming metabolism in roots.

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Analysis of the WUSCHEL-RELATED HOMEOBOX gene family in Pinus pinaster : New insights into the gene family evolution

Cited by 45 publications

References 72 publications

New Approaches to Optimize Somatic Embryogenesis in Maritime Pine

New Approaches to Optimize Somatic Embryogenesis in Maritime Pine

Control of root meristem establishment in conifers

Ultraviolet-B radiation induces cell death in root tips and reprograms metabolism in Arabidopsis

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