Analysis of the wheat and Puccinia triticina (leaf rust) proteomes during a susceptible host‐pathogen interaction

Abstract

Wheat leaf rust is caused by the fungus Puccinia triticina. The genetics of resistance follows the gene-for-gene hypothesis, and thus the presence or absence of a single host resistance gene renders a plant resistant or susceptible to a leaf rust race bearing the corresponding avirulence gene. To investigate some of the changes in the proteomes of both host and pathogen during disease development, a susceptible line of wheat infected with a virulent race of leaf rust were compared to mock-inoculated wheat using 2-DE (with IEF pH 4-8) and MS. Up-regulated protein spots were excised and analyzed by MALDI-QqTOF MS/MS, followed by cross-species protein identification. Where possible MS/MS spectra were matched to homologous proteins in the NCBI database or to fungal ESTs encoding putative proteins. Searching was done using the MASCOT search engine. Remaining unmatched spectra were then sequenced de novo and queried against the NCBInr database using the BLAST and MS BLAST tools. A total of 32 consistently up-regulated proteins were examined from the gels representing the 9-day post-infection proteome in susceptible plants. Of these 7 are host proteins, 22 are fungal proteins of known or hypothetical function and 3 are unknown proteins of putative fungal origin.

In Planta Proteomics and Proteogenomics of the Biotrophic Barley Fungal Pathogen Blumeria graminis f. sp. hordei>

“…There are published reports that analyze the leaf proteome of plants infected by pathogenic fungi, but few of these describe fungal proteins within total extracts from infected leaves, and only a very limited number of fungal proteins have been identified in these tissues (12,13). In one instance intercellular washing fluids were extracted to detect secreted fungal proteins in the apoplast (14).…”

A Signaling-Regulated, Short-Chain Dehydrogenase of Stagonospora nodorum Regulates Asexual Development

“…Previously, such approaches have been used to study phytopathogenic fungi through protein profiling (2,5,12,42,44) and to identify host- (45) and morphogenesis-responsive (25) proteins. Recent sequencing of the genomes of the phytopathogenic fungi Magnaporthe grisea, Ustilago maydis, Fusarium graminearum, and Stagonospora nodorum (7,9,19,24) provides an opportunity for more thorough mass spectrometry (MS)-based proteomic analyses (2,42).…”

The Uredinales: Cytology, Biochemistry, and Molecular Biology

“…Cooper et al (2006) analyzed proteins from urediospores of U. appendiculatus and indicated similar upcoming analyses for the germ tube stage and infection structures. Rampitsch et al (2006) have started to analyze the proteome of P. triticina during its interaction with its host. Integration of results from such approaches with array, EST, and genomic data will provide useful information to understand the molecular details of obligate biotrophy.…”