Analysis of the VERNALIZATION-A1 exon-4 polymorphism in polyploid wheat

Muterko, Alexandr; Салина, Е. А.

doi:10.18699/vj16.19-o

Cited by 13 publications

(17 citation statements)

References 24 publications

(47 reference statements)

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“…Almost all VRN-A1 alleles carrying mutations within the promoter region and intron-1 are characterized by the intact exon 7, Ex7C haplotype (Table 2), with the exception of the Vrn-A1c (type IL369) and Vrn-A1j alleles, previously identified in hexaploid wheat ( [23], GenBank: KU738894) and were not found in tetraploid species ( [12,25,26], here). [20] were characterized by the intact exon 7 of VRN-A1, confirming the Ex7C haplotype of VRN-D4. At the same time, in five accessions of T. sphaerococcum with VRN-D4 (PI 190982, PI 277142, PI 278650, PI 70711, PI 324492) both alternative haplotypes of VRN-A1 exon 7 were detected.…”

Section: Vrn-a1 Exon 7 Polymorphism In Polyploid Wheatmentioning

confidence: 53%

“…At the same time, in five accessions of T. sphaerococcum with VRN-D4 (PI 190982, PI 277142, PI 278650, PI 70711, PI 324492) both alternative haplotypes of VRN-A1 exon 7 were detected. However, the exclusion of the VRN-D4 haplotypes during multiplex AS-PCR with the Ex4outV4F2m3 forward primer, previously designed to avoid the amplification of VRN-D4 [20], indicated the Ex7T haplotype for the VRN-A1 gene in these accessions of T. sphaerococcum.…”

Section: Vrn-a1 Exon 7 Polymorphism In Polyploid Wheatmentioning

confidence: 94%

“…To denote the VRN-A1 exon 4 haplotype, the expanded nomenclature of the VRN-A1 haplotypes was also used [20]. With this nomenclature, mutations within VRN-A1 intron-4 distinguish Ex4C.s, Ex4C.m, Ex4C.f and Ex4C.sph haplotypes (Figure 3).…”

Section: Combinations Of the Vrn-a1 Exon 4 And Exon 7 Haplotypesmentioning

confidence: 99%

“…In our recent study, analysis of the distribution of the VRN-A1 exon 4 haplotypes in 12 tetraploid and hexaploid wheat species and in different VRN-A1 alleles, found the mutant type of exon 4 (Ex4T haplotype) only in hexaploid wheat with a recessive vrn-A1 allele, and only in combination with the intact exon 4 (Ex4C haplotype) of this gene (i.e., in accessions of hexaploid wheat carrying no less than two copies of VRN-A1 per genome) [20]. However, the polymorphism of the VRN-A1 exon 7 was previously analyzed only in some varieties of T. aestivum, but not other polyploid wheat species.…”

Section: Introductionmentioning

confidence: 99%

“…This problem was resolved in our recent study through design of allele-specific primers and the optimization of PCR for the identification of VRN-D4 and distinguishing between VRN-A1 and VRN-D4 haplotypes. Furthermore, the co-dominant DNA markers, based on the modulation of the curvature of the DNA molecule were designed to enable discrimination between the VRN-A1 exon 4 haplotypes [20].…”

Section: Introductionmentioning

confidence: 99%

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Origin and Distribution of the VRN-A1 Exon 4 and Exon 7 Haplotypes in Domesticated Wheat Species

Muterko

Салина

2018

Agronomy

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Abstract:The high adaptive potential of modern wheat to a wide range of environmental conditions is determined by genetic changes during domestication. Genetic diversity in VRN1 genes is a key contributor to this adaptability. Previously, the association between the transitions C->T within the fourth and seventh exons of VRN-A1, the distinguishing pair haplotypes Ex4C/Ex4T and Ex7C/Ex7T, and the modulation of such agronomically valuable traits as the vernalization requirement duration, frost tolerance and flowering time of wheat have been shown. However, this polymorphism was analyzed in only a few cultivars of Triticum aestivum L., and not in other wheat species. In the present study, VRN-A1 exon 4 and exon 7 were investigated in six tetraploid and five hexaploid wheat species carrying different VRN-A1 alleles. An allele-specific polymerase chain reaction (PCR) assay was optimized to identify the VRN-A1 exon 7 haplotypes. It was found that polymorphism of the VRN-A1 exon 7 originated in wild tetraploid wheat of Triticum dicoccoides Körn, while the mutant exon 4 of this gene originated later in domesticated hexaploid wheat of T. aestivum. Both these polymorphisms are found in all hexaploid wheat species. Analysis of the VRN-A1 exon 4 and exon 7 haplotype combinations found that intact exon 7 and mutant exon 4 are associated with analogous types of exon 4 and 7, respectively. With the exclusion of the Vrn-A1c (IL369) and Vrn-A1j alleles, identified only in hexaploid wheat, all dominant VRN-A1 alleles carry intact exons 4 and 7 (Ex4C/7C haplotype). The Ex4C/4T/7T haplotype was detected in numerous accessions of hexaploid wheat and is associated with the presence of multiple copies of VRN-A1. Overall, modern domesticated hexaploid wheat T. aestivum includes most possible combinations of the VRN-A1 exon 4 and exon 7 haplotypes among polyploid wheat, which are present in different proportions. This contributes to the high adaptive potential to a broad range of environmental conditions and facilitates the widespread distribution of this species throughout the world.

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Section: Vrn-a1 Exon 7 Polymorphism In Polyploid Wheatmentioning

confidence: 53%

Section: Vrn-a1 Exon 7 Polymorphism In Polyploid Wheatmentioning

confidence: 94%

Section: Combinations Of the Vrn-a1 Exon 4 And Exon 7 Haplotypesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Origin and Distribution of the VRN-A1 Exon 4 and Exon 7 Haplotypes in Domesticated Wheat Species

Muterko

Салина

2018

Agronomy

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VRN1-ratio test for polyploid wheat

Muterko

Салина

2019

Planta

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Characterization of the VRN-A1 allele introgressed from T. aestivum ssp. petropavlovskyi that influences the heading time in bread wheat

2023

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The optimal timing of the heading stage, mainly determined by the VRN genes, contributes to the wide spread of bread wheat and the realization of adaptive and breeding potential. Wild wheat species are valuable sources for expanding the bread wheat genetic diversity by the introgression of new gene alleles, including VRN genes. In this study, a near-isogenic line of the winter wheat cultivar Bezostaya 1 with a VRN-A1 dominant allele was obtained with a T. aestivum ssp. petropavlovskyi accession as the donor. Using known PCR markers for the promoter and rst intron sequences of the VRN-1 gene and subsequent sequencing of PCR fragments, the presence of the Langdon deletion was revealed in the rst intron (Vrn-A1L allele), previously described only for tetraploid wheat. The allele composition of VRN genes was determined in T. aestivum ssp. petropavlovskyi accessions and the presence of the Vrn-A1L dominant allele was established in all accessions. It was shown that the Vrn-A1L dominant allele increased the shoots-heading period under long-and shortday conditions, which is associated with a prolongation before the rst node formation. The comparative study of productivity characteristics of isogenic lines with Vrn-A1a and Vrn-A1Ldominant alleles on spike and plant productivity is presented.

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Analysis of the VERNALIZATION-A1 exon-4 polymorphism in polyploid wheat

Cited by 13 publications

References 24 publications

Origin and Distribution of the VRN-A1 Exon 4 and Exon 7 Haplotypes in Domesticated Wheat Species

Origin and Distribution of the VRN-A1 Exon 4 and Exon 7 Haplotypes in Domesticated Wheat Species

VRN1-ratio test for polyploid wheat

Characterization of the VRN-A1 allele introgressed from T. aestivum ssp. petropavlovskyi that influences the heading time in bread wheat

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