Analysis of the Transient Current in Nanopores Using a Circle-Median Filter

Abstract

To identify and analyze the transient current jump caused by the weak interaction between biomolecules in a nanopore experiment, CM Analyzer software was designed based on the Circle-Median algorithm. The algorithm effectively recognized falling events and outliers in nanopore data. To verify the accuracy of the developed software, a simulation signal with different noise (std: 0.5~2) was generated, and results showed that the software achieves data-processing requirements with a 250-kHz sampling rate and high noise. The algorithm was also used in α-hemolysin biological nanopore-based single DNA molecule detection experiments, and results showed that the software can accurately identify the falling current and rising transit in single nanopore blockages.