Analysis of the synergistic effect of glycyrrhizin and other constituents in licorice extract on lipopolysaccharide-induced nitric oxide production using knock-out extract

Uto, Takuhiro; Morinaga, Osamu; Tanaka, Hiroyuki; Shoyama, Yukihiro

doi:10.1016/j.bbrc.2011.11.143

Cited by 51 publications

(46 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…berberine [32] and 18b-glycyrrhetinic acid [33] had antileishmanial activity via MAPKinases, iNOS induction and NO up-regulation. However, they showed inhibitory effects on LPS-iNOS in macrophages [34,35].…”

Section: Discussionmentioning

confidence: 94%

Assessment of β-lapachone loaded in lecithin-chitosan nanoparticles for the topical treatment of cutaneous leishmaniasis in L. major infected BALB/c mice

Moreno

Schwartz

Larrea

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

View full text Add to dashboard Cite

Patients affected by cutaneous leishmaniasis need a topical treatment which cures lesions without leaving scars. Lesions are produced not only by the parasite but also by an uncontrolled and persistent inflammatory immune response. In this study, we proposed the loading of β-lapachone (β-LP) in lecithin-chitosan nanoparticles (NP) for targeting the drug to the dermis, where infected macrophages reside, and promote wound healing. The loading of β-LP in lecithin-chitosan NP was critical to achieve important drug accumulation in the dermis and permeation through the skin. In addition, it did not influence the drug antileishmanial activity. When topically applied in L. major infected BALB/c mice, 2 β-LP NP achieved no parasite reduction but they stopped the lesion progression. Immuno-histopatological assays in CL lesions and quantitative mRNA studies in draining lymph nodes confirmed that β-LP exhibited anti-inflammatory activity leading to the downregulation of IL-1β and COX-2 expression and a decrease of neutrophils infiltrate.

show abstract

Section: Discussionmentioning

confidence: 94%

Assessment of β-lapachone loaded in lecithin-chitosan nanoparticles for the topical treatment of cutaneous leishmaniasis in L. major infected BALB/c mice

Moreno

Schwartz

Larrea

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

View full text Add to dashboard Cite

show abstract

“…Equal amounts of lysate protein were subjected to SDS-PAGE . The proteins were electrotransferred to PVDF membranes and detected, as described previously (Uto et al, 2012). We used corosolic acid (CA) then as positive control (Uto et al, 2013).…”

Section: Western Blot Analysismentioning

confidence: 99%

Berberine Induces Apoptotic Cell Death via Activation of Caspase-3 and -8 in HL-60 Human Leukemia Cells: Nuclear Localization and Structure–Activity Relationships

et al. 2017

Self Cite

View full text Add to dashboard Cite

Berberine (BBR), an isoquinoline alkaloid, is a well-known bioactive compound contained in medicinal plants used in traditional and folk medicines. In this study, we investigated the subcellular localization and the apoptotic mechanisms of BBR were elucidated. First, we confirmed the incorporation of BBR into the cell visually. BBR showed antiproliferative activity and promptly localized to the nucleus from 5 min to 15 min after BBR treatment in HL-60 human promyelocytic leukemia cells. Next, we examined the antiproliferative activity of BBR (1) and its biosynthetically related compounds (2-7) in HL-60 cells. BBR exerted strongest antiproliferative activity among 1-7 and the results of structures and activity relation suggested that a methylenedioxyl group in ring A, an O-alkyl group at C-9 position, and the frame of isoquinoline may be necessary for antiproliferative activity. Moreover, BBR showed the most potent antiproliferative activity in HL-60 cells among human cancer and normal cell lines tested. Next, we examined the effect of BBR on molecular events known as apoptosis induction. In HL-60 cells, BBR induced chromatin condensation and DNA fragmentation, and triggered the activation of PARP, caspase-3 and caspase-8 without the activation of caspase-9. BBR-induced DNA fragmentation was abolished by pretreatment with inhibitors against caspase-3 and caspase-8, but not against caspase-9. ERK and p38 were promptly phosphorylated after 15 min of BBR treatment, and this was correlated with time of localization to the nucleus of BBR. These resultsCorrespondence to: Prof. Yukihiro Shoyama, Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan. Tel: (þ81) The American Journal of Chinese Medicine, Vol. 45, No. 7, 1497-1511 demonstrated that BBR translocated into nucleus immediately after treatments and induced apoptotic cell death by activation of caspase-3 and caspase-8.

show abstract

“…Therefore, if we take too much licorice extracts for promotion of fertilization, such disease may be occurred. Previously, we purified glycyrrhizin from the crude extract of licorice using an immunoaffinity column conjugated with anti-glycyrrhizin monoclonal antibody, and prepared glycyrrhizin-knockout extract [17]. The glycyrrhizin-knockout extract contains all components except glycyrrhizin.…”

Section: Licorice In Futurementioning

confidence: 99%

“…In order to control the quality of licorice, we investigated and succeeded the preparation of monoclonal antibody (MAb) against glycyrrhizin and set up an enzyme-linked immunosorbent assay (ELISA) as quick, simple and reproducible assay system [14]. Furthermore, a new staining system, eastern blotting was developed [15] and immunoaffinity isolation of glycyrrhizin resulting in glycyrrhizin-knockout extract, which can be used to survey the real activity of glycyrrhizin in licorice [16,17]. Regarding flavonoid, anti-liquiritin MAb [18] and eastern blotting [19] was developed.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Fertilization Activators for Future

Tanaka¹,

Wada²,

Tung³

et al. 2017

Biological Activities and Action Mechanisms of Licorice Ingredients

Self Cite

View full text Add to dashboard Cite

Artificial insemination is an indispensable technology for cattle breeding and is used for treating infertility in humans. Thus, new or improved methods are needed to increase the efficiency of artificial insemination. In vitro fertilization (IVF) has been developed in mice. Although many mouse lines produced using IVF have been preserved by freezing embryos and/or fertilized eggs, the more efficient IVF using freezing preservation or long-term refrigeration of sperm is expected to preserve mouse lines more easily. In this chapter, we introduce the active compounds in licorice to improve the rate of IVF. We previously reported that the rate of IVF in mice was improved by adding a water extract of licorice (Glycyrrhiza uralensis), but not glycyrrhizin, to the artificial insemination culture medium. Recently, we analyzed the active ethyl acetate fraction containing high levels of flavonoids. This fraction was further purified by bioassay-guided separation to isolate isoliquiritigenin and formononetin, which contributed to the improved rate of IVF. Isoliquiritigenin and formononetin may be useful therapeutic agents for infertility treatment.

show abstract

Analysis of the synergistic effect of glycyrrhizin and other constituents in licorice extract on lipopolysaccharide-induced nitric oxide production using knock-out extract

Cited by 51 publications

References 28 publications

Assessment of β-lapachone loaded in lecithin-chitosan nanoparticles for the topical treatment of cutaneous leishmaniasis in L. major infected BALB/c mice

Assessment of β-lapachone loaded in lecithin-chitosan nanoparticles for the topical treatment of cutaneous leishmaniasis in L. major infected BALB/c mice

Berberine Induces Apoptotic Cell Death via Activation of Caspase-3 and -8 in HL-60 Human Leukemia Cells: Nuclear Localization and Structure–Activity Relationships

In Vitro Fertilization Activators for Future

Contact Info

Product

Resources

About