Analysis of the signal cross talk via CCL26 in the tumor microenvironment in osteosarcoma

Abstract

Interaction with surrounding healthy cells plays a major role in the growth and metastasis of osteosarcoma. In this study, we hypothesized that humoral factors, which do not require direct contact with cells, are involved in the interaction between osteosarcoma and the surrounding cells. We identified the humoral factor involved in the association between tumor cells and surrounding normal cells using a co-culture model and investigated the significance of our findings. When human osteosarcoma cells (MG63) and human mesenchymal stem cells (hMSCs) were co-cultured and comprehensively analyzed for changes in each culture group, we found that the expression of chemokine (CC motif) ligand 26 (CCL26) was significantly enhanced. We also analyzed the changes in cell proliferation in co-culture, enhanced interaction with administration of recombinant CCL26 (rCCL26), reduced interaction with administration of anti-CCL26 antibodies, changes in invasive and metastatic abilities. CCL26 levels, motility, and invasive capability increased in the co-culture group and the group with added rCCL26, compared to the corresponding values in the MG63 single culture group. In the group with added CCL26 neutralizing antibodies, CCL26 level decreased in both the single and co-culture groups, and motility and invasive ability were also reduced. In a nude mice lung metastasis model, the number of lung metastases increased in the co-culture group and the group with added rCCL26, whereas the number of tumors were suppressed in the group with added neutralizing antibodies compared to those in the MG63 alone. This study identified a possible mechanism by which osteosarcoma cells altered the properties of normal cells to favorably change the microenvironment proximal to tumors and to promote distant metastasis.

Differences in systemic immune parameters in individuals with lung cancer according to race

“…(9) Conversely, CCL8, CXCL1, and CCL26-all significantly higher in Black patients in the present study-promote tumorigenesis and cancer progression in various malignancies. (10)(11)(12) Results from the analysis of immune cell populations appear to correlate with our cytokine findings. We observed higher levels of nonclassical monocytes and NKT-like cells in Black patients.…”

“…(9) Conversely, CCL8, CXCL1, and CCL26—all significantly higher in Black patients in the present study—promote tumorigenesis and cancer progression in various malignancies. (10-12)…”

Surface roughness modulates EGFR signaling and stemness of triple-negative breast cancer cells

“…Eotaxin-3 (CCL26), IL-9, TSLP, and Fas were differentially expressed (DE) on surfaces with Ra = 1.5 µm. These factors are responsible for the stimulation of immune cells to sites of inflammation ( Goswami and Kaplan, 2011 ; Kawano et al, 2021 ) and immune cell death ( Volpe et al, 2016 ), and cancer has been associated with immune privilege and increased invasiveness and metastasis ( Abrahams et al, 2003 ; Lan et al, 2018 ; Das et al, 2021 ). Likewise, MDA-MB-231 cells cultured on surfaces with Ra = 1.5 µm showed enriched bioprocesses for inflammation ( Figure 2C ).…”

CCL22 and CCL26 are potential biomarkers for predicting distant metastasis in thyroid carcinoma

“…Meanwhile, they also showed an increased number of lung metastases in a lung metastasis model in nude mice in which recombinant CCL26 was added. 25 Another study demonstrated that CCL26 induces tumor-associated macrophage infiltration by binding to the CCL26 receptor in colorectal cancer. 26 Furthermore, hypoxia is a common feature of solid tumors, and CCL26 can be activated by hypoxia-inducible factors.…”