Analysis of the saccharification capability of high-functional cellulase JN11 for various pretreated biomasses through a comparison with commercially available counterparts

Abstract

Although the capabilities of Trichoderma reesei cellulases have been greatly improved, these enzymes are still too costly for commercial use. The aim of this research was to assess the biomass saccharification capability of JN11, a recombinant cellulase, compared with that of the commercially available cellulases Accellerase 1500 and Cellic CTec. The activities of JN11, Accellerase 1500, and Cellic CTec were compared by using various types of cellulosic biomass, including rice straw, Erianthus, eucalyptus, and Japanese cedar. JN11 had higher saccharification capability for rice straw, Erianthus, eucalyptus, and Japanese cedar compared with the commercial cellulases. The JN11 saccharification of cellulosic biomasses, including hemicellulose (NaOH-pretreated biomasses), resulted in high glucose and xylose yields because of the high xylanase/xylosidase activity of JN11. Moreover, even JN11 saccharification of hemicellulose-free biomasses (sulfuric acid-, hydrothermally, and steam exploded-pretreated biomasses) resulted in high glucose yields. The cellulase activity of JN11, however, was comparable to that of its commercial counterparts. These findings indicate that the saccharification ability of cellulase is unrelated to its cellulase activity when measured against Avicel, CMC, pNP-lactoside, and other substrates. JN11 showed high activity for all types of pretreated cellulosic biomasses, indicating its usefulness for saccharification of various cellulosic biomasses.

Utilization of recombinant Trichoderma reesei expressing Aspergillus aculeatus β-glucosidase I (JN11) for a more economical production of ethanol from lignocellulosic biomass

“…From the specific activity data of the prepared enzyme used in this study, we found that JN11 has a greater BGL activity while the endo-and exo-cellulase activities were maintained and were comparable to the enzyme from its parental strain, PC-3-7 (Table S1). This is in accordance with the finding of Nakazawa et al (20) and Kawai et al (21), who reported that JN11 has higher saccharification capability for various pretreated biomass compared to that of PC-3-7 or other commercial enzymes. JN11 has since been considered as a promising enzyme for efficient ethanol production from lignocellulosic material.…”

Line monitoring by near-infrared chemometric technique for potential ethanol production from hydrothermally treated Eucalyptus globulus

“…Moreover, a negative band at 5980 cm −1 , attributable to aromatic framework vibrations, was detected, and the band relating to lignin at 6900 cm −1 also was negative. These results indicated that pretreatments that resulted in lower hemicellulose and higher lignin contents increased the saccharification ratio, which is consistent with a report published by our co-author Kawai [25]. These discussions on the regression coefficient can be summarized as follows: the hydrothermal treatment preferentially removes hemicellulose and results in higher lignin contents, as already reported [26,27], which leads to hydrophobic properties.…”

Identification of Major Facilitator Transporters Involved in Cellulase Production during Lactose Culture ofTrichoderma reeseiPC-3-7

“…26) Due to its high cellulase productivity, PC-3-7 is also used industrially to enhance lignocellulosic biomass y To whom correspondence should be addressed. Tel: +81-258-47-9407; Fax: +81-258-47-9400; E-mail: owataru@vos.nagaokaut.ac.jp conversion 28,29) and to reduce the cost of saccharification. Because several PC-3-7 derivative strains are being used industrially, the next focus of our research is optimization of strain cultivation parameters in order to improve cellulase production further.…”