Analysis of the rumen bacteria and methanogenic archaea of yak (Bos grunniens) steers grazing on the Qinghai-Tibetan Plateau

Xue, Dan; Chen, Huai; Chen, Fang; He, Yixin; Zhao, Chunrong; Zhu, Dan; Zeng, Lile; Li, Wei

doi:10.1016/j.livsci.2016.04.009

Cited by 67 publications

(38 citation statements)

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“…This also explained the significant changes of Firmicutes in the three stages in the QTP. As in other studies of yak grazed on QTP, the three most abundant microbial phyla were Firmicutes, Bacteroidetes, and Proteobacteria (Dan et al 2016;Xue et al 2017). However, in the current study, third most abundant phylum was verrucomictobia (5.97%), followed by Proteobacteria (1.92%).…”

Section: Discussionsupporting

confidence: 48%

“…To enhance the fiber digestibility and feed utilization, it is necessary to obtain a deep understanding of the microorganisms in the rumen. Firmicutes and uncultured species were reported to have higher abundance in the yak of the QTP compared to yak at low elevation, and several bacteria (Ruminococcus albus and Prevotella ruminicola) were either not detected or had lower abundance among fibrolytic bacteria (Ruminococcus, Fibrobacter, Clostridium, and Butyrivibrio) in the yaks of the QTP (Yang et al 2010;Dan et al 2016;Huang et al 2017;Xue et al 2017;Xue et al 2018). Previous studies have shown that factors such as diet, age, species, and seasons all impact on ruminal microbes, and diet is a particularly important contributor (Tajima et al 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Diet and environmental conditions influence the microbial community composition; however, little is known about the bacterial community changes over time when ruminants are on similar diets, especially in the QTP where the forage differs considerably throughout a whole year due to the harshness of the environment. Some studies have been conducted on rumen microbiota in grazing yaks and sheep in QTP (Dan et al 2016;Huang et al 2017;Guo et al 2018). Changes in temperature and precipitation directly affect plant yak productivity, which in turn affects animal growth and the microbiome in the body.…”

Section: Introductionmentioning

confidence: 99%

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Peer Review #2 of "Yak rumen microbial diversity at different forage growth stages of an alpine meadow on the Qinghai-Tibet Plateau (v0.1)"

2019

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The rumen microbiota of ruminants plays a vital role in fiber digestion, and environmental factors affect its community structure. The yak (Bos grunniens) is the main livestock species that inhabits the Qinghai-Tibet Plateau (QTP) at regions located at high-altitude of 3000-5000 m. This work investigated the rumen bacterial community of yak that grazed on the QTP during the whole year to evaluate the relationship between the rumen bacterial community and the nutrient composition of forage plant at three stages. In this study, the diversity of the rumen prokaryotic community composition was monitored in 10 fullgrazing yak in an alpine meadow of the QTP. The nutrient composition of three forage growth stages was determined: re-green stage (REGY), grassy stage (GY), and withered stage (WGY). High-throughput sequencing of bacterial 16S rRNA gene was used. The results showed that the nutritive composition of the alpine meadow changed with the seasons: crude protein (CP) (13.22%) was high in forage during REGY (spring), while neutral detergent fiber (NDF) (59.00%) was high during WGY (winter). Microbial diversity and richness were highest during REGY and the average number of operational taxonomic units from 30 samples was 4470. The microbial composition was dominated by members of Bacteroidetes (51.82%), followed by Firmicutes (34.08%), and the relative microbial abundance changed in the three forage growth stages. Unweighted UniFrac distance PcoA showed that the bacterial community structure differed between REGY, GY, and WGY. Furthermore, taxonomic groups did not present differences regarding gender in these three stages. The rumen microbiota was enriched with functional potentials that were related to ABC transporters, the Two component system, Aminoacyl-tRNA biosynthesis, and metabolism of Purine, Pyrimidine, Starch and sucrose metabolism. Significant differences were found in the composition, diversity, and function of yak ruminal microorganisms during different forage growth stages. This indicates that microbial changes in the rumen depend on changes in the forage nutritional composition. These

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Section: Discussionsupporting

confidence: 48%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Peer Review #2 of "Yak rumen microbial diversity at different forage growth stages of an alpine meadow on the Qinghai-Tibet Plateau (v0.1)"

2019

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“…These bacteria grow only under anaerobic conditions. The rumen fluid contains anaerobic bacteria, one of them methanogenic bacteria [10,11]. The number of anaerobic bacteria in the rumen reaches 10 11 Cell/g.…”

Section: Introductionmentioning

confidence: 99%

The potential of various livestock waste as sources of methanogenic bacteria

Marlina

Kurnani

Hidayati

et al. 2018

J. Pow. Tech. Adv. Funct. Mater.

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Livestock waste contains organic matter as nutrients for methanogenic bacteria. However, the limiting factor was the acidogens and acetogens continuously produce compounds that reduce the pH of the system below the preferred range for methanogens to survive if sufficient buffering capacity is not available.The objective of this research is to determine the potency of various livestock waste such as dairy cattle waste and beef cattle waste, as a source of methanogens. The technique for counting bacteria was through Total Plate Count in anaerobic Hungate tube. The incubation period was 30 days. Methane was observed using Gas Chromatography (GC) analysis and the concentration of VFAs was measured using titration method. The results showed that methane production was directly proportional to the growth of anaerobic bacteria. Based on the growth of anaerobic bacteria and the production of methane in the media indicated that the dairy cattle waste and beef cattle waste were potential as a source of methanogenic bacteria.

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“…1975). The effect of diet on the structure of rumen microbial communities has been widely investigated in Yak, Tan sheep, Holstein dairy cows, Mehshana buffalo and goats (Pitta et al 2014;Jiao et al 2015;Pitta et al 2016;Wang et al 2016;Xue et al 2016). However, as the dominant ruminant and living foundation for local herdsman, the studies of Tibetan sheep have only focused on the growth performance, slaughter performance and economic benefit under different dietary supplementation (Baruah et al 2012;Chen et al 2015c;Dodd et al 2011;Feng et al 2013;Lee et al 2012;Xu et al 2017), but few comprehensive studies focused on the growth performance, rumen fermentation and rumen microbial communities of Tibetan sheep.…”

Section: Introductionmentioning

confidence: 99%

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Supplemental Information 7: Clean Data of Tibetan Sheep in Groups HS4

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Analysis of the rumen bacteria and methanogenic archaea of yak (Bos grunniens) steers grazing on the Qinghai-Tibetan Plateau

Cited by 67 publications

References 67 publications

Peer Review #2 of "Yak rumen microbial diversity at different forage growth stages of an alpine meadow on the Qinghai-Tibet Plateau (v0.1)"

Peer Review #2 of "Yak rumen microbial diversity at different forage growth stages of an alpine meadow on the Qinghai-Tibet Plateau (v0.1)"

The potential of various livestock waste as sources of methanogenic bacteria

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