Analysis of the root-knot nematode (Meloidogyne incognita) resistance and Ma gene cloning in Xinjiang wild (Prunus sogdiana) myrobalan plums

Xiao, Kun; Zhu, Haifeng; Zhu, Xiang; Hu, Kui; Hu, Jing

doi:10.1016/j.scienta.2019.108772

Cited by 1 publication

(1 citation statement)

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“…2.9 | Detecting the degree of resistance after inoculation with M. incognita Tobacco roots were washed with water, and the disease index was graded 60 days after inoculation, according to Xiao et al (2010). Progression of the disease (percentage of roots with galls vs the total number of root numbers) was classified as follows: 0, no galls; grade 1, 1%-10% galls; grade 2, 11%-20% galls; grade 3, 21%-50% galls; grade 4, 51%-80% galls; and grade 5, > 80% galls.…”

Section: Genetic Transformation Of Transgenic Tobaccomentioning

confidence: 99%

Cloning and functional analysis of the root‐knot nematode resistance gene NtRk1 in tobacco

Jiang

Chen

et al. 2023

Physiologia Plantarum

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Tobacco (Nicotiana tabacum L.) is an economically important crop worldwide. Root‐knot nematodes (RKNs) are responsible for yield losses in tobacco and other crops, such as tomato, potato, peanut, and soybean. Therefore, screening for resistance genes that can prevent RKN infestation and the associated damage is crucial. However, there is no report of cloning tobacco RKN resistance genes to date. Here, we cloned the tobacco RKN resistance gene NtRk1 from the resistant variety TI706, using rapid amplification of cDNA ends. NtRk1 has high homology with other RKN resistance genes (CaMi in pepper, Mi‐1.1 and Mi‐1.2 in tomato). Under normal conditions, NtRk1 was barely expressed in the roots; however, following RKN infection, its expression level rapidly increased. Overexpression of NtRk1 in the susceptible cultivar “Changbohuang” enhanced its resistance to Meloidogyne incognita, while RNA interference of NtRk1 in the resistant cultivar K326 resulted in its susceptibility to M. incognita. Moreover, compared with resistant variety K326, we found the salicylic acid and jasmonic acid contents of RNAi plants decreased after inoculation with M. incognita, and confirmed that the function of NtRk1 is related to these phytohormones. These findings indicate that NtRk1 is an RKN resistance gene, which is abundantly expressed in response to RKN infection and may enhance host defense responses by elevating salicylic acid and jasmonic acid levels.

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Section: Genetic Transformation Of Transgenic Tobaccomentioning

confidence: 99%