Background: In China, grass-roots village doctors, as the gatekeepers of rural residents' health, play an important role in guaranteeing and serving the health level of rural residents. However, the serious burnout and high turnover rate of village doctors threaten the stability of village medical teams. This research evaluated the influence of personality, alexithymia and work engagement on the burnout of village doctors in an in-depth and detailed way, and explored the mediating role of work engagement and alexithymia between personality and the burnout of village doctors in China.Methods: During May and June 2019, 2,684 rural doctors in 1,345 rural clinics in Jining city, Shandong Province, were studied using a quantitative study self-administered questionnaire. The questionnaire included demographic and sociological characteristics, personality, alexithymia, work engagement and burnout, and was measured by 5-7 Likert scale. One-way ANOVA, Person correlation analysis, and Structural Equation Modelling (SEM) were used for statistical analysis.Results: Up to 54.3% of the surveyed rural doctors showed moderate to severe emotional exhaustion, 90.5% of them had moderate to severe sense of low personal achievement and 33.9% of them had moderate to severe depersonalization burnout. Personality has a direct positive effect on work engagement (β=0.50, p<0.001), but has a direct negative effect on alexithymia (β=-0.52, p<0.001) and burnout (β=-0.50, p<0.001) respectively. Work engagement has a direct negative effect on burnout (β=-0.10, p<0.001), while alexithymia has a direct positive effect on burnout (β=0.16, p<0.001). In the path between personality and burnout, both work engagement, 95%CI:(−0.17)–(−0.08), and alexithymia 95%CI:(−0.36)–(−0.09), have significant mediating effects.The above results strongly confirmed that personality, alexithymia, and work engagement were early, powerful predicators of burnout.Conclusion: According to the results, medical administrators should pay attention to the personality characteristics of village doctors in their professional training, practice selection and job assignment and encourage doctors to actively reflect on their own personality and to reduce job burnout by obtaining necessary social support, constructing reasonably achievable career expectations, improving time management and participating in psychological counselling programs.