Analysis of the reversibility of biliary cirrhosis in young rats submitted to biliary obstruction

Braz, Maria Julia de Aro; Corbi, Leonardo Ervolino; Tannuri, Ana Cristina Aoun; Coelho, Maria Cecília Mendonça; Gonçalves, Josiane Oliveira; Serafini, Suellen; Tannuri, Uenis

doi:10.1016/j.jpedsurg.2017.08.012

Cited by 9 publications

(6 citation statements)

References 14 publications

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“…de Aro Braz et al and Corbi et al previously established a rat model of CJS to study the effect of related indexes of biliary cirrhosis after removal of biliary obstruction. However, the specific method of CJS was common bile duct duodenal anastomosis (25,26). However, at present, the anastomosis used in clinical work is Roux-en-Y CJS.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of scar formation following Roux-en-Y choledochojejunostomy in a novel rat model of obstructive jaundice

Lyu¹,

Wang²,

Zhou³

et al. 2021

Ann Transl Med

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Background:The present study aimed to analyze the scar formation mechanism following Roux-en-Y choledochojejunostomy (CJS) in a novel rat model of obstructive jaundice. Methods:The biliary obstruction model of Sprague-Dawley (SD) rats was established in advance, and 24 rats were randomly divided into 4 groups (control group, 1-day ligation group, 3-day ligation group, and 5-day ligation group). Changes in postoperative weight, common bile duct diameter, and laboratory indexes were analyzed to determine the best operation time. Roux-en-Y CJS in rats was studied based on the model, and the rats were randomly divided into 4 groups [control group, 3-day choledochojejunostomy (CJS) group, 7-day CJS group, and 30-day CJS group]. The same indexes were analyzed, and the characteristics of scar formation were evaluated by histopathology and polymerase chain reaction examination.Results: The third day after common bile duct ligation is the best time for a Roux-en-Y CJS. The common bile duct diameter expands to 4.2 mm on average, and these physiological characteristics are consistent with current standard clinical findings. After completing CJS, the rats' weight returned to normal levels, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TB), direct bilirubin (DB), and C-reactive protein (CRP) indexes gradually decreased (P<0.05). Anastomotic stoma diameter tended to narrow with time and was significantly narrower on day 30 than preoperation. After CJS, the expression of α-smooth muscle actin (α-SMA) peaked in the early stage and was still higher than that of the control group in the bile duct wall 1 month postoperatively (P<0.05). Transforming growth factor-β1 (TGF-β1) expression gradually increased and was higher than that of the control group at each stage postoperatively (P<0.05). Conclusions:The rat Roux-en-Y CJS model is more in line with our surgical model, and the clinical condition has potential applicability for the study of CJS scar formation. Scar formation following CJS in rats is characterized by the activation of fibroblasts caused by early inflammatory stimulation, which leads to the proliferation of collagen and smooth muscle fibers, resulting in scars.

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Section: Discussionmentioning

confidence: 99%

Mechanism of scar formation following Roux-en-Y choledochojejunostomy in a novel rat model of obstructive jaundice

Lyu¹,

Wang²,

Zhou³

et al. 2021

Ann Transl Med

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“…Recibido: 21 de julio 2023 Aceptado: 4 de septiembre 2023 DOI: https://doi.org/10.47307/GMC.2023.131.4.10 ORCID: 0000-0003-0059-2715 1 ORCID:0000-0001-6372-2092 2 ORCID:0000-0002-5229-0068 3 ORCID:0000-0002-2803-799 4 ORCID:0000-0002-2690-8995 5 ORCID:0000-0001-6262-6795 6 ORCID:0000-0001-5058-1251 7 experimental. Método: Se utilizaron varios enfoques metodológicos, entre ellos el experimental, el bioquímico y el de comparación.…”

Section: Resumen Objetivo: El Trabajo Tuvo Como Objetivo Establecer L...unclassified

“…Considerable attention has been paid to this question in scientific doctrine, in particular, it has been actively studied by various specialists. Despite this, in connection with the deterioration of the external conditions of the development and vital activities of society, the question of the effect on the liver of the incomplete recovery of the outflow of bile, which is formed to a greater extent by the natural course of the disease or is achieved by therapeutic procedures, has become especially relevant (2)(3)(4)(5). Obstructive cholestasis resulting from benign or malignant disease frequently complicates clinical outcomes through adverse hepatic changes.…”

Section: Introductionmentioning

confidence: 99%

Histological Liver Changes in Experimental Obstructive Cholestasis with Partial Outflow Restoration

Mamontov

2023

Gac Méd Caracas

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Objective: This work aimed to establish histological changes in the structure and activity of the liver caused by experimental obstructive cholestasis. Method: Several methodological approaches were used such as experimental, biochemical, and comparison were applied.Results: It was possible to describe the essence of such a disease as cholestasis and its impact on the liver. At the same time, it was proved that recanalization with incomplete recovery of bile outflow was possible due to the normalization of the total bilirubin (TB) level. Morphological changes in the liver were most pronounced during acute cholangitis. The study examined what processes occur in the body during the recovery of bile flow, including after the complete blockage of such organs as the liver and bile ducts. Consequently, signs that expressed the abnormality of the liver structure of animals were revealed.

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“…The group of de Aro Braz et al performed CBD ligations in Wistar rats with subsequent biliary drainage procedures, focusing on changes in the histological and molecular profile. 35,36 Studies on drug-induced cholestasis in animal models remained limited: in 1983, Ogawa et al used 1,4-phenylenediisothiocyanate in rats, and in 1991, Schmeling et al used a phorbol myristate acetate infusion into the biliary tract. These methods resulted in cellular necrosis of the biliary duct; however, the model showed heterogeneous results.…”

Section: Pre-and Postnatal Induction Of Cholestasis In Animal Modelsmentioning

confidence: 99%

Translational Research in Biliary Atresia: News from Mice and Men

Madadi-Sanjani

Petersen

2019

Eur J Pediatr Surg

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Biliary atresia (BA) is a fibro-obliterative cholangiopathy of unknown etiology. While Kasai portoenterostomy achieves temporary biliary drainage in some cases, BA remains the most common indication for liver transplantation during childhood. During the last few decades, observations on BA, like cholestatic diseases in animals and the introduction of different animal models for BA, have not achieved the anticipated results, and we are still not able to translate the basic research to the patient's bedside. This article presents a review of the literature on available BA animal models and gives a glimpse of future developments.

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Analysis of the reversibility of biliary cirrhosis in young rats submitted to biliary obstruction

Cited by 9 publications

References 14 publications

Mechanism of scar formation following Roux-en-Y choledochojejunostomy in a novel rat model of obstructive jaundice

Mechanism of scar formation following Roux-en-Y choledochojejunostomy in a novel rat model of obstructive jaundice

Histological Liver Changes in Experimental Obstructive Cholestasis with Partial Outflow Restoration

Translational Research in Biliary Atresia: News from Mice and Men

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