Analysis of the Relationship Between Enzymatic and Antiviral Activities of the Chicken Oligoadenylate Synthetase-Like

Tag-EL-Din-Hassan, Hassan T.; Sasaki, Nobuya; Torigoe, Daisuke; Morimatsu, Masami; Agui, Takashi

doi:10.1089/jir.2016.0012

Cited by 11 publications

(13 citation statements)

References 33 publications

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“…These observations further support the idea of species-specific adaptation of avian OASL in which UBL domain contributes to dsRNA binding, and thus effective synthesis of 2 -5 A. Furthermore, in contrast to duck OASL (duOASL), deletion of two C-terminal UBL domains of chOASL-A led to abrogation of 2 -5 A synthesis, indicating that the impact of UBL domains on dsRNA binding affinity is much higher with chOASL compared to its duck counterpart [142]. In addition, when conserved D residues were mutated to abolish 2 -5 A synthesizing activity and RNase L activation, duOASL antiviral activity switched to activate RIG-I [137].…”

Section: Oaslsupporting

confidence: 78%

Avian Pattern Recognition Receptor Sensing and Signaling

Neerukonda

Katneni

2020

Veterinary Sciences

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Pattern recognition receptors (PRRs) are a class of immune sensors that play a critical role in detecting and responding to several conserved patterns of microorganisms. As such, they play a major role in the maintenance of immune homeostasis and anti-microbial defense. Fundamental knowledge pertaining to the discovery of PRR functions and their ligands continue to advance the understanding of immune system and disease resistance, which led to the rational design and/or application of various PRR ligands as vaccine adjuvants. In addition, the conserved nature of many PRRs throughout the animal kingdom has enabled the utilization of the comparative genomics approach in PRR identification and the study of evolution, structural features, and functions in many animal species including avian. In the present review, we focused on PRR sensing and signaling functions in the avian species, domestic chicken, mallard, and domestic goose. In addition to summarizing recent advances in the understanding of avian PRR functions, the present review utilized a comparative biology approach to identify additional PRRs, whose functions have been well studied in mammalians but await functional characterization in avian.

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Section: Oaslsupporting

confidence: 78%

Avian Pattern Recognition Receptor Sensing and Signaling

Neerukonda

Katneni

2020

Veterinary Sciences

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“…Activation of the classical OAS/RNase L pathway results in RNA degradation of both viral and cellular RNA, including ribosomal RNA within infected cells. Subsequently, it inhibits protein translation in these cells, which leads to effective prevention of viral genome replication [131,132]. The expression of OAS is increased following viral infections, including IBV [96].…”

Section: Interferon-stimulated Genes (Isgs)mentioning

confidence: 99%

Antiviral responses against chicken respiratory infections: Focus on avian influenza virus and infectious bronchitis virus

2020

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“…WNV genomic RNA harboring the secreted alkaline phosphatase (SEAP) reporter gene instead of viral structural genes, WNV/SEAP-replicon RNA was propagated as described previously (Moritoh, et al, 2011;Tag-EL-Din-Hassan, et al, 2017). BHK-21 cells were cultured in a 100-mm plate at the density of 1.5 × 10 6 cells/plate for 24 h and then, 10 µg of pIRES-EGFP (empty vector as the control), pduOASL-FL-EGFP, pgoOASL-FL-EGFP, posOASL-FL-EGFP, posOAS1-FL-EGFP, and pchOASL-A-FL-EGFP were transfected using Lipofectamine 2000 ® (Invitrogen) according to the manufacturer's protocol.…”

Section: Inhibitory Activity On Wnv Replicon Replicationmentioning

confidence: 99%

Functional analysis of duck, goose, and ostrich 2′-5′-oligoadenylate synthetase

Tag-EL-Din-Hassan

Morimatsu

Agui

2018

Infection, Genetics and Evolution

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Up-to-date the flavivirus infection in avian taxa is not clearly defined. Several reports have demonstrated that many viruses belonging to Flaviviridae may cause diseases in poultry species; however, the susceptibility of other avian species is variable and still unclear. In human and mice, the 2'-5'-oligoadenylate synthetase (OAS) proteins are associated with resistance to the flavivirus infection as well as other virus infections. However, the avian OAS proteins are rarely studied. In our previous studies, we confirmed that the chicken OAS-like protein (chOASL) expressed OAS-enzymatic activity (the classical OAS/RNase L-dependent pathway) as well as the anti-flavivirus activity (the putative OAS/RNase L-independent pathway). Therefore, the current study aimed at functional analysis of avian OAS proteins from duck, goose, and ostrich. The duOASL, goOASL, and osOAS1 proteins expressed enzymatic activity as well as chOASL, whereas osOASL expressed little enzymatic activity. On the other hand, duOASL, goOASL, and osOASL possessed significant antiviral activity against West Nile virus (WNV)-replicon replication as well as chOASL, whereas osOAS1 did not. In addition, similar to chOASL, their antiviral activity was independent of RNase L activation. These results suggest that OASL is the only OAS protein in the duck and goose as well as chicken and possesses both OAS-enzymatic and anti-flavivirus activities, whereas the ostrich possesses both OAS1 and OASL proteins with sharing the functional activities, OAS-enzymatic and anti-flavivirus activities, respectively. It is of interest that the ostrich undergoes differential process in OAS gene evolution from other poultries and thus possesses different molecular mechanism in antiviral activity.

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Analysis of the Relationship Between Enzymatic and Antiviral Activities of the Chicken Oligoadenylate Synthetase-Like

Abstract: 11revealed that all mutated proteins showed no enzymatic activity except for ChOAS-L-AΔUbL2. 12However, all mutated proteins showed antiviral activity to inhibit the replication of the WNV

Cited by 11 publications

References 33 publications

Avian Pattern Recognition Receptor Sensing and Signaling

Avian Pattern Recognition Receptor Sensing and Signaling

Antiviral responses against chicken respiratory infections: Focus on avian influenza virus and infectious bronchitis virus

Functional analysis of duck, goose, and ostrich 2′-5′-oligoadenylate synthetase

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