Analysis of the Proteinaceous Components of the Organic Matrix of Calcitic Sclerites from the Soft Coral Sinularia sp.

Rahman, M. Azizur; Shinjo, Ryuichi; Oomori, Tamotsu; Wörheide, Gert

doi:10.1371/journal.pone.0058781

Cited by 13 publications

(13 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we used 1D and 2D electrophoresis as well as MALDI‐TOF‐TOF MS to characterize the proteinaceous components of the sclerites and axial skeletons of the precious coral Corallium konojoi . Our analyses revealed a highly complex mixture of proteins in both skeletons, and we determined that several proteins were glycosylated, similar to what has been reported for other octocorals . Glycoproteins are thought to be very important for calcification and are commonly found in calcifying systems .…”

supporting

confidence: 85%

Characterization of the proteinaceous skeletal organic matrix from the precious coralCorallium konojoi

et al. 2014

Self Cite

View full text Add to dashboard Cite

The Japanese red and pink corals are known to be precious because of their commercial value resulting from their use in ornaments, jewelry, and medicine. Precious corals are very interesting models for biomineralization studies and possess two different skeletal structures: an axial skeleton and an endoskeleton (sclerites). Although it has long been known that the organic matrix proteins existing in coral skeletons are critical for the oriented precipitation of CaCO3 crystals, these proteins in moderate deep-sea Japanese precious corals remain uncharacterized. Therefore, in this study, we performed skeletal whole proteome analyses using 1D and 2D electrophoresis, nano-LC, and MALDI-TOF-TOF MS. We identified a total of 147 functional coral skeletal organic matrix proteins (120 from the sclerites and 36 from the axial skeleton), including two calcium-binding calmodulin. Among the organic matrix proteins identified, nine key proteins are highly typical and common in both skeletons. Strong glycosylation activity, which is essential for skeletal formation in calcifying organisms, was detected in both skeletons. This work demonstrates unique biomineralization-related proteins in precious corals and provides the first description of the major proteinaceous components of CaCO3 minerals in precious corals, enabling the comparative investigation of biocalcification in other octocorals.

show abstract

supporting

confidence: 85%

Characterization of the proteinaceous skeletal organic matrix from the precious coralCorallium konojoi

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…The XRD analysis (Figure ) also revealed differences between the UA, UB, UC, UD and UE group samples. The mineral formed in UA and UB samples displayed peaks at 2theta values of 23.2, 29.5, 36.1, 39.5, 43.3, 47.7 and 48.6 which are characteristic for calcite (Rahman et al ., ) (see the Supplementary material online, Figure S1). In samples incubated in UC and UD, the peak at 29.5, characteristic of the (104) plane of calcite, shifted to 29.8, which is characteristic of magnesian calcite (Diaz‐Pulido et al ., ).…”

Section: Resultsmentioning

confidence: 99%

Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications

Douglas

Łapa

Samal

et al. 2017

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Mineralization of hydrogel biomaterials is considered desirable to improve their suitability as materials for bone regeneration. Calcium carbonate (CaCO ) has been successfully applied as a bone regeneration material, but hydrogel-CaCO composites have received less attention. Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated. In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO , Mg-enriched CaCO and magnesium carbonate to generate composite biomaterials for bone regeneration. Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions. Increasing the magnesium concentration decreased mineral crystallinity. At low magnesium concentrations calcite was formed, while at higher concentrations magnesian calcite was formed. Hydromagnesite (Mg (CO ) (OH) .4H O) formed at high magnesium concentration in the absence of calcium. The amount of mineral formed and compressive strength decreased with increasing magnesium concentration in the mineralization medium. The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media. Mineralization of hydrogels with calcite or magnesian calcite promoted adhesion and growth of osteoblast-like cells. Hydrogels mineralized with hydromagnesite displayed higher cytotoxicity. In conclusion, enzymatic mineralization of GG hydrogels with CaCO in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect. Copyright © 2017 John Wiley & Sons, Ltd.

show abstract

“…Interestingly, other metazoans constructing calcified skeletons have also been found to contain actin in their skeletal matrices even after rigorous washing of powdered skeleton, for example, the stony coral Stylophora pistillata ( Drake et al 2013a , 2013b ; Mass et al 2014 ), and the sclerites of the calcitic octocoral Sinularia sp. ( Rahman et al 2013 ), indicating that actin may genuinely have some biomineralization-related function in these organisms.…”

Section: Resultsmentioning

confidence: 99%

The Magellania venosa Biomineralizing Proteome: A Window into Brachiopod Shell Evolution

Jackson

Mann

Häussermann

et al. 2015

Genome Biology and Evolution

Self Cite

View full text Add to dashboard Cite

Brachiopods are a lineage of invertebrates well known for the breadth and depth of their fossil record. Although the quality of this fossil record attracts the attention of paleontologists, geochemists, and paleoclimatologists, modern day brachiopods are also of interest to evolutionary biologists due to their potential to address a variety of questions ranging from developmental biology to biomineralization. The brachiopod shell is a composite material primarily composed of either calcite or calcium phosphate in close association with proteins and polysaccharides which give these composite structures their material properties. The information content of these biomolecules, sequestered within the shell during its construction, has the potential to inform hypotheses focused on describing how brachiopod shell formation evolved. Here, using high throughput proteomic approaches and next generation sequencing, we have surveyed and characterized the first shell-proteome and shell-forming transcriptome of any brachiopod, the South American Magellania venosa (Rhynchonelliformea: Terebratulida). We find that the seven most abundant proteins present in the shell are unique to M. venosa, but that these proteins display biochemical features found in other metazoan biomineralization proteins. We can also detect some M. venosa proteins that display significant sequence similarity to other metazoan biomineralization proteins, suggesting that some elements of the brachiopod shell-forming proteome are deeply evolutionarily conserved. We also employed a variety of preparation methods to isolate shell proteins and find that in comparison to the shells of other spiralian invertebrates (such as mollusks) the shell ultrastructure of M. venosa may explain the effects these preparation strategies have on our results.

show abstract

Analysis of the Proteinaceous Components of the Organic Matrix of Calcitic Sclerites from the Soft Coral Sinularia sp.

Cited by 13 publications

References 55 publications

Characterization of the proteinaceous skeletal organic matrix from the precious coralCorallium konojoi

Characterization of the proteinaceous skeletal organic matrix from the precious coralCorallium konojoi

Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications

The Magellania venosa Biomineralizing Proteome: A Window into Brachiopod Shell Evolution

Contact Info

Product

Resources

About