Analysis of the Overlapped Electrochemical Signals of Hydrochlorothiazide and Pyridoxine on the Ethylenediamine-Modified Glassy Carbon Electrode by Use of Chemometrics Methods

Abstract

In this work, the electrochemical behavior of hydrochlorothiazide and pyridoxine on the ethylenediamine-modified glassy carbon electrode were investigated by differential pulse voltammetry. In pH 3.4 Britton-Robinson (B-R) buffer solution, both hydrochlorothiazide and pyridoxine had a pair of sensitive irreversible oxidation peaks, that overlapped in the 1.10 V to 1.20 V potential range. Under the optimum experimental conditions, the peak current was linearly related to hydrochlorothiazide and pyridoxine in the concentration range of 0.10–2.0 μg/mL and 0.02–0.40 μg/mL, respectively. Chemometrics methods, including classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS), were introduced to resolve the overlapped signals and determine the two components in mixtures, which avoided the troublesome steps of separation and purification. Finally, the simultaneous determination of the two components in commercial pharmaceuticals was performed with satisfactory results.

Development of a Versatile, Low-Cost Electrochemical System to Study Biofilm Redox Activity at the Micron Scale

“…As the UME approaches the surface, the decreasing gap between the UME and the surface hinders diffusion to the UME surface. While ferrocene species, including ferrocene methanol (FcMeOH), are nontoxic to some cells, the presence of these mediators means that other molecules, such as H 2 O 2 , cannot be measured accurately in their presence due to overlapping current signals ( 40 ). Thus, there is a need to develop alternative methods for approaching the surface of biofilms.…”