Analysis of the Oral Hypoglycemic Agent, Glibenclamide, in a Health Food

Abstract

Glibenclamide, a sulfonylurea derivative (SU) antidiabetic agent was detected in a health food by three diŠerent methods: TLC, HPLC, and liquid chromatography-mass spectrometry (LC MS). For analysis of SU antidiabetics, the sample was extracted with acetone as a sample solution. TLC analysis of the sample solution showed a speciˆc spot that had the same characteristics as those of glibenclamide standard solution. HPLC analysis monitored using a photo-diode array detector showed that the sample solution had a peak with a unique UV spectrum, with coincided with that of standard glibenclamide. In sample solution, LC MS analysis in positive and negative modes indicated that the (M＋H) ＋ and (M-H) -ions occurred at m/z 494 and m/z 492, respectively. These results indicate that the monoisotopic mass is 493, coincident with that of glibenclamide. Quantitative HPLC analysis showed that the glibenclamide content in the health food was 0.78 mg/capsule (1.55 mg/g of sample contents). Because the initial dosage of glibenclamide for diabetics is 1.25-2.5 mg per day, this health food has su‹cient medicinal eŠect and also has the potential to cause adverse eŠects.

Screening and Quantitative Analysis for Sulfonylurea-Type Oral Antidiabetic Agents in Adulterated Health Food Using Thin-Layer Chromatography and High-Performance Liquid Chromatography

“…GLB is commonly used as an oral antidiabetic drug and is sometimes detected in health food. 4) Each powdered solution of GLB tablet was equivalent in weight to one tablet. These samples were independently analyzed with the TLC and HPLC methods.…”

“…On the other hand, health food advertising weight loss, hypoglycemic effects, and the improvement of sexual dysfunction have a growing market share. Some products are adulterated with synthetic drugs, [1][2][3][4] although natural or herbal materials are stated to be the ingredients. Over the past few years in Japan, several cases have been reported concerning unexpected adverse effects when consumers have consumed health food adulterated with synthetic drugs.…”

“…As previously reported, glibenclamide, a sulfonylurea-type oral antidiabetic agent (SU-OAD), was detected in a health food product advertising a hypoglycemic effect. 4) However, there have been few reports concerning analytical methods for health food adulterated with synthetic drugs. For the analysis of SU-OADs, a number of reports have described analytical meth- Screening and quantitative analysis for six sulfonylurea-type oral antidiabetic agents (SU-OADs), tolbutamide (TOL), acetohexamide (ACE), chlorpropamide (CHL), gliclazide (GLC), glibenclamide (GLB), and glimepiride (GLM), in adulterated health food has been developed.…”

Rapid Analysis of an Additive Medicinal Ingredient (Dried Thyroid) in Dietary Supplements

“…1,2) To prevent impairment of health by medicinal ingredients contained in dietary supplements, it is necessary to analyze commercial dietary supplements as thoroughly as possible. Analytical methods are generally required to be highly sensitive, simple, rapid, and low in cost.…”

Investigation on the Interactions between Various Drugs and Aojiru (Green Juice) Using a Simple Centrifugation Method

“…Under such circumstances, unfavorable effects from the consumption of health foods and dietary supplements have been noted. 1,2) Moreover, undesirable pharmacokinetic or pharmacodynamic interactions have been reported with various drugs. [3][4][5][6][7] We also demonstrated that kurosu (Japanese unpolished rice vinegar) is capable of modulating the intestinal absorption of P-glycoprotein (P-gp) substrates.…”