Analysis of the molecular interaction of glycosylated proteins with rabbit liver asialoglycoprotein receptors using surface plasmon resonance spectroscopy

Terada, T.; Nishikawa, Makiya; Yamashita, Fumiyoshi; Hashida, Mitsuru

doi:10.1016/j.jpba.2006.01.054

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…Therefore, 2min interval between the intravenous injections of Gal-BSA and Gal-PEG-ODN is reasonable for the competition study. Systemic administration of excess Gal-BSA is known to saturate the asialoglycoprotein receptor of the hepatocytes (30). Prior administration of Gal-BSA at a dose of 10mg/Kg caused a significant decrease in the hepatic uptake of Gal-PEG-33 P-ODN, but increase in the radioactivity in plasma and other organs at 30 min post injection.…”

Section: Competition In Hepatic Uptake Of Gal-peg-33 P-odnmentioning

confidence: 99%

Site-Specific Delivery of Oligonucleotides to Hepatocytes after Systemic Administration

Zhu

Cheng

et al. 2007

Bioconjugate Chem.

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We previously complexed ODN with galactosylated poly (L-lysine) (Gal-PLL) to enhance its site specific delivery to hepatocytes. To avoid the use of polycations, in this study we conjugated galactosylated poly (ethylene glycol) (Gal-PEG (MW of PEG: 3486±500Da)) to ODN via an acid labile ester linkage of β-thiopropionate. Following tail vein injection into rats, Gal-PEG-33 P-ODN rapidly cleared from the circulation and 60.2% of the injected dose accumulated in the liver at 30 min post injection, which was significantly higher than that deposited after injection of 33 P-ODNs. The plasma concentration versus time profile of Gal-PEG-33 P-ODN was biphasic, with 4.38±0.36 min as t 1/2 of distribution and 118.61±22.06 min as t 1/2 of elimination. Prior administration of excess Gal-BSA decreased the hepatic uptake of Gal-PEG-33 P-ODN from 60.2% to 35.9%, suggesting galactose triggers the asialoglycoprotein receptor-mediated endocytosis of Gal-PEG-33 P-ODN by hepatocytes. A large proportion of the injected Gal-PEG-33 P-ODN was taken up by the hepatocytes as evidence by determination of radioactivity in the digested liver cells upon liver perfusion and separation by centrifugation on a Nycodenz gradient. In conclusion, Gal-PEG-ODN conjugate may be used for treating a variety of liver diseases.

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Section: Competition In Hepatic Uptake Of Gal-peg-33 P-odnmentioning

confidence: 99%

Site-Specific Delivery of Oligonucleotides to Hepatocytes after Systemic Administration

Zhu

Cheng

et al. 2007

Bioconjugate Chem.

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“…Therefore, 2 min interval between the intravenous injections of Gal-BSA and Gal-PEG-ODN is reasonable for the competition study. Systemic administration of excess Gal-BSA is known to saturate the asialoglycoprotein receptor of the Hepatocytes (203). Prior administration of Gal-BSA at a dose of 10 mg/Kg caused a significant decrease in the hepatic uptake of Gal-PEG-33 P-ODN, but increase in the radioactivity in plasma and other organs at 30 min post injection.…”

Section: P-odnmentioning

confidence: 99%

Design of Lipid and Polymeric Carriers for Nucleic Acid Delivery

Zhu¹

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Survey of the year 2006 commercial optical biosensor literature

Rich

Myszka

2007

J of Molecular Recognition

109

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We identified 1219 articles published in 2006 that described work performed using commercial optical biosensor platforms. It is interesting to witness how the biosensor market is maturing with an increased number of instrument manufacturers offering a wider variety of platforms. However, it is clear from a review of the results presented that the advances in technology are outpacing the skill level of the average biosensor user. While we can track a gradual improvement in the quality of the published work, we clearly have a long way to go before we capitalize on the full potential of biosensor technology. To illustrate what is right with the biosensor literature, we highlight the work of 10 groups who have their eye on the ball. To help out the rest of us who have the lights on but nobody home, we use the literature to address common myths about biosensor technology.

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Analysis of the molecular interaction of glycosylated proteins with rabbit liver asialoglycoprotein receptors using surface plasmon resonance spectroscopy

Cited by 5 publications

References 29 publications

Site-Specific Delivery of Oligonucleotides to Hepatocytes after Systemic Administration

Site-Specific Delivery of Oligonucleotides to Hepatocytes after Systemic Administration

Design of Lipid and Polymeric Carriers for Nucleic Acid Delivery

Survey of the year 2006 commercial optical biosensor literature

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