Analysis of the microRNA expression profiles of chicken dendritic cells in response to H9N2 avian influenza virus infection

Yang, Jing; Huang, Xinmei; Liu, Yuzhuo; Zhao, Dong; Han, Kyung-Nam; Zhang, Lijiao; Li, Yin; Liu, Qingtao

doi:10.1186/s13567-020-00856-z

Cited by 11 publications

(9 citation statements)

References 36 publications

(40 reference statements)

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“…It can also detect miRNAs with very low copy numbers, which reflect either a low expression level in the tissue or a contribution from minor cell types such as blood or adipose or connective tissues in the biopsy specimen [ 39 – 41 ]. In this experiment, the number of up-regulated miRNAs was more than down-regulated miRNAs, which was consisted with the other researches of miRNA sequencing in chicken [ 42 , 43 ]. The pathways for up-regulated miRNA target genes were cell apoptosis, nucleic acid metabolism, regulation of protein metabolism, glycolysis/gluconeogenesis, PPAR signaling pathway and other metabolic-related processes, the down-regulated miRNA target genes were mainly enriched in Toll -like receptor signaling pathway, which was the immune-related process.…”

Section: Discussionmentioning

confidence: 99%

Regulation of mRNA and miRNA in the response to Salmonella enterica serovar Enteritidis infection in chicken cecum

Miao

Liu

et al. 2022

BMC Vet Res

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Background Salmonella enterica, serovar Enteritidis (SE) is a food-borne pathogen, which can cause great threat to human health through consumption of the contaminated poultry products. Chicken is the main host of SE. The mRNA and microRNA (miRNA) expression profiles were analyzed on cecum of Shouguang chicken via next-generation sequencing and bioinformatics approaches. The treated group was inoculated SE, and the control group was inoculated with phosphate buffer saline (PBS). Results There were 1760 differentially expressed mRNAs in the SE-infected group, of which 1046 were up-regulated mRNA, and 714 were down-regulated mRNA. In addition, a total of 821 miRNAs were identified, and 174 miRNAs were differentially expressed, of which 100 were up-regulated and 74 were down-regulated. Functional enrichment of differentially expressed mRNAs was similar to miRNA target genes. The functional analysis results of differentially expressed mRNAs and miRNAs were performed. Immune-related processes and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways were enriched by up-regulated mRNA. The down-regulated mRNAs were enriched in tissue development and metabolic-related KEGG pathways. The functional analysis of up-regulated miRNA target genes was similar to the down-regulated mRNAs. The down-regulated miRNA target genes were enriched in metabolic-related GO (Gene Ontology) -BP (Biological process) terms and KEGG pathways. The overlap of the up-regulated mRNA and the up-regulated miRNA target genes (class I) was 325, and the overlap of the down-regulated miRNA target genes (class II) was 169. The class I enriched in the immune-related GO-BP terms and KEGG pathways. The class II mainly enriched in metabolic-related GO-BP terms and KEGG pathways. Then we detected the expression of mRNA and miRNA through qRT-PCR. The results shown that the expression of HHIP, PGM1, HTR2B, ITGB5, RELN, SFRP1, TCF7L2, SCNN1A, NEK7, miR-20b-5p, miR-1662, miR-15a, miR-16-1-3p was significantly different between two groups. Dual-luciferase reporter assay was used to detect the relationship between miR-20b-5p and SCNN1A. The result indicated that miR-20b-5p regulate immune or metabolic responses after SE infection in Shouguang chickens by directly targeting SCNN1A. Conclusions The findings here contribute to the further analysis of the mechanism of mRNA and miRNA defense against SE infection, and provide a theoretical foundation for the molecular disease-resistant breeding of chickens.

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Section: Discussionmentioning

confidence: 99%

Regulation of mRNA and miRNA in the response to Salmonella enterica serovar Enteritidis infection in chicken cecum

Miao

Liu

et al. 2022

BMC Vet Res

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“…It is generally accepted that innate immunity and adaptive immunity are both important in the prevention of chicken coccidiosis 10 . However, in the early stages of infection, the innate immunity of the host ensures an immediate response to invading parasites, and activation of innate immune responses primarily depends on innate immune receptors 11 . Toll‐like receptors (TLRs) are major components of innate immune system in vertebrate species, and these transmembrane proteins are responsible for microbial recognition.…”

Section: Introductionmentioning

confidence: 99%

“…10 However, in the early stages of infection, the innate immunity of the host ensures an immediate response to invading parasites, and activation of innate immune responses primarily depends on innate immune receptors. 11 Toll-like receptors (TLRs) are major components of innate immune system in vertebrate species, and these transmembrane proteins are responsible for microbial recognition. Once activated by specific ligands such as bacterial lipopolysaccharide (LPS), glycolipids, viral nucleic acids and CpG Oligodeoxynucleotides (CpG ODN), TLR signals can lead to the recruitment of various immune cells and generate a wide range of biologically active molecules including chemokines, cytokines and antimicrobial peptides, which stimulates the innate immune responses necessary for parasite clearance and resistance to re-infection.…”

mentioning

confidence: 99%

Expression of Toll‐like receptors and host defence peptides in the cecum of chicken challenged with Eimeria tenella

Wang,

Bai

et al. 2024

Parasite Immunology

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Chicken coccidiosis, caused by Eimeria protozoa, affects poultry farming. Toll‐like receptors (TLRs) and host defence peptides (HDPs) help host innate immune responses to eliminate invading pathogens, but their roles in Eimeria tenella infection remain poorly understood. Herein, 14‐day‐old chickens were treated orally with 50,000 E. tenella oocysts and the cecum was dissected at different timepoints. mRNA expression of 10 chicken TLRs (chTLRs) and five HDPs was measured by quantitative real‐time PCR. chTLR7 and chTLR15 were upregulated significantly at 3 h post‐infection while other chTLRs were downregulated (p < .05). chTLR1a, chTLR1b, chTLR2b and chTLR4 peaked at 36 h post‐infection, chTLR3, chTLR5 and chTLR15 peaked at 72 h post‐infection and chTLR21 expression was highest among chTLRs, peaking at 48 h post‐infection (p < 0.05). For HDPs, cathelicidin (CATH) 1 to 3 and B1 peaked at 48 h post‐infection, liver‐expressed antimicrobial peptide 2 peaked at 96 h post‐infection, and CATH 2 expression was highest among HDPs. CATH2 and CATH3 were markedly upregulated at 3 h post‐infection (p < .05). The results provide insight into innate immune molecules during E. tenella infection in chicken, and indicate that innate immune responses may mediate resistance to chicken coccidiosis.

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“…One early study reported DCs can be grown by co-culturing chicken bone marrow (BM)derived cells with chicken granulocyte-macrophage stimulating factor (GM-CSF) and chicken interleukin 4 (IL-4) [30]. In this study, we culture bone-derived chicken dendritic cells (ck-BM-DC) to examine gene expression levels of IFN-α, TLR-3, TLR-7, MHC-I, IL-1β, IL-6, Mx, Casp-3, and Casp-8 of DCs infected with AIV There are limited studies examining the interactions between chicken DCs and AIV [25,[31][32][33]. The exact nature of how AIV infections affect DCs is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Morphologic characterization and cytokine response of chicken bone-marrow derived dendritic cells to infection with high and low pathogenic avian influenza virus

Mo,

Segovia,

Chrzastek

et al. 2024

Preprint

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Dendritic cells (DCs) are professional antigen-presenting cells, which are key components of the immune system and involved in the early immune response. DCs are specialized in capturing, processing, and presenting antigens to facilitate immune interactions. Chickens infected with avian influenza virus (AIV) demonstrate a wide range of clinical symptoms, based on pathogenicity of the virus. Low pathogenic avian influenza (LPAI) viruses typically induce mild clinical signs, whereas high pathogenic avian influenza (HPAI) induce more severe disease, which can lead to death within days. For this study, chicken bone marrow-derived DC (ckBM-DC)s were produced and infected with high and low pathogenic avian influenza viruses of H5N2 or H7N3 subtypes to characterize innate immune responses, study effect on cell morphology, and evaluate virus replication. A strong proinflammatory response, including chicken interleukin-1β, and stimulation of the interferon response pathway were observed at 8 hours post infection. Microscopically, the DCs underwent morphological changes from classic elongated dendrites to a more general rounded shape that eventually lead to cell death with the presence of scattered cellular debris. Differences in onset of morphologic changes were observed between H5 and H7 subtypes. Increases in viral titers demonstrated that both HPAI and LPAI are capable of infecting and replicating in DCs. The elevated expression of infected DCs may be indicative with a dysregulation of the immune response typically seen with HPAI infections.

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Analysis of the microRNA expression profiles of chicken dendritic cells in response to H9N2 avian influenza virus infection

Cited by 11 publications

References 36 publications

Regulation of mRNA and miRNA in the response to Salmonella enterica serovar Enteritidis infection in chicken cecum

Regulation of mRNA and miRNA in the response to Salmonella enterica serovar Enteritidis infection in chicken cecum

Expression of Toll‐like receptors and host defence peptides in the cecum of chicken challenged with Eimeria tenella

Morphologic characterization and cytokine response of chicken bone-marrow derived dendritic cells to infection with high and low pathogenic avian influenza virus

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