Analysis of the microbiota of refrigerated chopped parsley after treatments with a coating containing enterocin AS-48 or by high-hydrostatic pressure

Grande, María José López; Aguayo, María del Carmen López; Pulido, Rubén Pérez; Gálvez, Antonio; Lucas, Rosario

doi:10.1016/j.foodres.2017.05.011

Cited by 7 publications

(8 citation statements)

References 39 publications

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“…The dressing was stable for the first 2 days, but it showed signs of deterioration (off-odor and color change) by day 5. In a previous study carried out by our group with chopped parsley stored in trays, the concentrations of total mesophilic aerobes increased from 6.3 at time 0 to 9.4 log CFU/g after 10 days of storage [ 20 ], values that are much higher than those achieved in the present study for the dressing samples packaged in sealed bags. This could indicate that the packaging conditions during storage are important for the preservation of the product.…”

Section: Discussioncontrasting

confidence: 76%

“…The counts of Enterobacteriaceae ranged widely from a few samples (3%) with counts of around 2 log CFU per gram, to others (16%) reaching counts of 7–8 log CFU per gram [ 18 ]. Other studies have reported initial microbial load values of total aerobic mesophiles between 3.0 and 5.0 log CFU/g for parsley [ 19 ], and 6.3 for chopped parsley [ 20 ]. For coriander, viable cell counts between 6.7 [ 21 ] and 7.0 log CFU/g have been reported [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Resistance, Biocide Tolerance, and Bacterial Diversity of a Dressing Made from Coriander and Parsley after Application of Treatments Using High Hydrostatic Pressure Alone or in Combination with Moderate Heat

López

Grande

Pulido

et al. 2022

Foods

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The effects of high-hydrostatic pressure (HP) treatments (450 and 600 megapascals, MPa, for 5 min at temperatures of 22 °C and 50 °C) on the microbiota of a coriander and parsley dressing was studied via culture-dependent and culture-independent approaches. Samples were refrigerated for 20 days, with periodic counts of the culture media supplemented with, or without, antimicrobials. HP-treated samples showed significantly lower viable cell counts compared to untreated controls. Only the control samples yielded bacterial growth on media with antimicrobials (imipenem, cefotaxime, benzalkonium chloride), including mostly Pseudomonas and Lactobacillus. Bacillus and Paenibacillus were identified from pressurized samples. Few isolates showed higher tolerance to some of the biocides tested. Pseudomonads showed outstanding resistance to meropenem and ceftazidime. According to high-throughput sequencing analysis, the microbiota of the dressing control samples changes during storage, with a reduction in the relative abundance of Proteobacteria and an increase in Firmicutes. The composition of the residual microbiota detected during storage was highly dependent on the pressure applied, and not on the treatment temperature.

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Section: Discussioncontrasting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance, Biocide Tolerance, and Bacterial Diversity of a Dressing Made from Coriander and Parsley after Application of Treatments Using High Hydrostatic Pressure Alone or in Combination with Moderate Heat

López

Grande

Pulido

et al. 2022

Foods

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“…were treated with 600 MPa for 25 min, showing a 4.5 log CFU/g microbial reduction ( Barba et al, 2017 ). In addition, Grande Burgos et al (2017) studied the HPP influence on the reduction of mesophilic aerobic bacteria and yeasts and molds in peaches. After the HPP treatment of 600 MPa for 8 min, mesophilic aerobic bacteria, yeasts, and molds were reduced on average by 2.9 log CFU/mL.…”

Section: New Developed Disinfection Methodsmentioning

confidence: 99%

Conventional and non-conventional disinfection methods to prevent microbial contamination in minimally processed fruits and vegetables

Mendoza

Luna

Pozo

et al. 2022

LWT

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“…The sequence of the V3–V4 region of 16S rRNA gene was used as the taxonomic basis to estimate bacterial populations present in the samples (Caporaso et al, 2011 ) using Illumina technology essentially as described elsewhere (Grande Burgos et al, 2017 ). DNA template was adjusted to 10–12 ng in each PCR reaction.…”

Section: Methodsmentioning

confidence: 99%

Treatment With High-Hydrostatic Pressure, Activated Film Packaging With Thymol Plus Enterocin AS-48, and Its Combination Modify the Bacterial Communities of Refrigerated Sea Bream (Sparus aurata) Fillets

Blázquez¹,

Grande²,

Pulido³

et al. 2018

Front. Microbiol.

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The aim of this study was to determine the impact of activated plastic films with thymol and enterocin AS-48 and high-hydrostatic pressure (HP) treatment on the bacterial load and bacterial diversity of vacuum-packaged sea bream fillets under refrigerated storage for 10 days. The activated film and the HP treatment reduced aerobic mesophiles viable counts by 1.46 and 2.36 log cycles, respectively, while the combined treatment achieved a reduction of 4.13 log cycles. HP and combined treatments resulted in longer delays in bacterial growth. Proteobacteria were the dominant phyla in sea bream fillets. The relative abundance of Firmicutes increased by the end of storage both in controls and in samples treated by HP singly or in combination with the activated films. The predominant operational taxonomic units (OTUs) found at time 0 in control samples (Listeria, Acinetobacter, Pseudomonas, Enterobacteriaceae, Chryseobacterium) rapidly changed during storage (with an increase of Vibrio, Photobacterium, and Shewanella together with Cloacibacterium and Lactobacillales by the end of storage). The activated film and the HP treatment induced drastic changes in bacterial diversity right after treatments (with Comamonadaceae, Methylobacterium, Acidovorax, and Sphingomonas as main OTUs) and also induced further modifications during storage. Bacterial diversity in activated film samples was quite homogeneous during storage (with Vibrio, Photobacterium, and Shewanella as main OTUs) and approached control samples. HP treatments (singly or in combination with activated films) determined a high relative abundance of Acinetobacter (followed by Pseudomonas and Shewanella) during early storage as well as a higher relative abundance of lactic acid bacteria by the end of storage. The results indicate that the complex dynamics of bacterial populations in the refrigerated sea bream fillets are markedly influenced by treatment and antimicrobials applied.

show abstract

Analysis of the microbiota of refrigerated chopped parsley after treatments with a coating containing enterocin AS-48 or by high-hydrostatic pressure

Cited by 7 publications

References 39 publications

Antimicrobial Resistance, Biocide Tolerance, and Bacterial Diversity of a Dressing Made from Coriander and Parsley after Application of Treatments Using High Hydrostatic Pressure Alone or in Combination with Moderate Heat

Antimicrobial Resistance, Biocide Tolerance, and Bacterial Diversity of a Dressing Made from Coriander and Parsley after Application of Treatments Using High Hydrostatic Pressure Alone or in Combination with Moderate Heat

Conventional and non-conventional disinfection methods to prevent microbial contamination in minimally processed fruits and vegetables

Treatment With High-Hydrostatic Pressure, Activated Film Packaging With Thymol Plus Enterocin AS-48, and Its Combination Modify the Bacterial Communities of Refrigerated Sea Bream (Sparus aurata) Fillets

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