Analysis of the Metabolites in Apical Area of Allium cepa Roots by High Resolution NMR Spectroscopy Method

Budantsev, A. Yu.; Uversky, Vladimir N.; Kutyshenko, V. P.

doi:10.2174/092986610789909476

Cited by 8 publications

(11 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The biological processes involved in cellular expansion include water uptake, nitrogen mobilization, increased sugar synthesis, plasma and tonoplast membrane flexibility etc. (Budentsev et al 2010;GonzalezReyes et al 1999). Plant roots interact with each of the physical factors in their environment and this interaction can lead to modification of the roots.…”

Section: Discussionmentioning

confidence: 99%

“…Plant roots interact with each of the physical factors in their environment and this interaction can lead to modification of the roots. Metabolites such as ascorbate and enzymes such as asparagine synthase and membrane ATPases have been described as promoters and mediators of these biological processes (Budentsev et al 2010). Alterations in these biological processes, including disrupted lipid biosynthesis by plant extracts and toxins have been linked to reduced cell wall expansibility, loss of vacuolar homeostatic regulation, cellular cytotoxicity, cell necrosis and root growth inhibition (Gonzalez-Reyes et al 1999).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Sumitha

Thoppil

2015

Cytotechnology

View full text Add to dashboard Cite

Hyptis suaveolens and Leucas indica, two common weeds were selected for the present study, to reveal their probable cytotoxic potential. The meristematic root tips of Allium cepa were used for testing the cytotoxic property of the aqueous leaf extracts containing both polar and non-polar compounds, and that containing polar compounds alone, at different concentrations (0.125, 0.25, 0.5, 1 and 2 %) and at different time durations, using distilled water as negative control. Mitotic squash preparations were made using a standard protocol. The mitotic index of the treated root tip cells was found to be decreasing and the abnormality percentage was found to be increasing with increase in extract concentration when compared with the control. Maximum cytotoxicity was observed in the extract containing both polar and non-polar compounds. Both the tested plants were found to be cytotoxic. The abnormalities noticed were of both clastogenic (nuclear lesions, nuclear fragmentation, etc.) and non-clastogenic (aberrant cell wall formation at cytokinesis, ball metaphase, etc.) types. Both plant extracts were found to significantly (P \ 0.05) inhibit root growth of Allium cepa with an EC 50 value of 1.92 % (R 2 = 0.594) for Hyptis and 1.58 % (R 2 = 0.757) for Leucas. The results were also analysed statistically by using analysis of variance followed by appropriate post hoc tests. These two weeds are aromatic plants comprising of essential oils that are volatile, natural complex compounds characterized by a strong odour and formed as secondary metabolites. In nature, essential oils play an important role in the protection of the plants as insecticides by reducing their appetite for such plants. When specifically targeted the concept of effectively exploiting these weeds for the formulation of herbal insecticides/ pesticides may be possible in the near future.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Sumitha

Thoppil

2015

Cytotechnology

View full text Add to dashboard Cite

show abstract

“…Under these conditions, deuterium can be incorporated to the substrates due to the existence of efficient exchange between the protons of organic moieties of substrates and deuterium present in media. Next, these deuterated substrates can be used in biochemical reactions leading to the enzymatic incorporation of deuterium to the corresponding metabolism products (Ewy, Ackerman & Balaban, 1988; Kushner, Baker & Dunstall, 1999; Budantsev, Uversky & Kutyshenko, 2010; Molchanov et al, 2012). One of the most informative techniques to follow the mentioned processes in biological fluids is the high-resolution NMR at the deuterium nuclei, 2 H-NMR (Budantsev, Uversky & Kutyshenko, 2010).…”

Section: Introductionmentioning

confidence: 99%

Looking at microbial metabolism by high-resolution²H-NMR spectroscopy

Kutyshenko

Beskaravayny

Молчанов

et al. 2013

PeerJ

View full text Add to dashboard Cite

We analyzed the applicability of high-resolution 2H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D2O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics.

show abstract

“…], the influence of various physicochemical condi tions on the growth and differentiation processes in apical meristem cells [11][12][13], etc. are currently actively studied today.In previous work, we attempted to use high resolu tion NMR spectroscopy to study metabolites in homogenates of apical cells of onion roots [14]. This paper presents the results of the study of the possibility of using NMR spectroscopy for a comparative study of root apical fragments of onion (homogenate extract) and the apex of intact roots.…”

mentioning

confidence: 99%

“…In previous work, we attempted to use high resolu tion NMR spectroscopy to study metabolites in homogenates of apical cells of onion roots [14]. This paper presents the results of the study of the possibility of using NMR spectroscopy for a comparative study of root apical fragments of onion (homogenate extract) and the apex of intact roots.…”

mentioning

confidence: 99%

From fragments to morphogenesis: NMR spectroscopy of metabolites in the apex of the roots of onion

Молчанов

Kutyshenko

Budantsev

et al. 2012

Dokl Biochem Biophys

View full text Add to dashboard Cite

Investigation of the mechanisms of morphogenesis is one of the central problems of biology. From the viewpoint of biophysics, the process of morphogenesis is reduced to the problem of assembling the whole from its fragments [1]. It can be assumed that, in the process of morphogenesis taking place under adverse environment impacts, adaptation proceeds from the bottom up. The lower levels involved in the assembly of the whole are adapted better; as a result of this adap tation, assembly at the upper levels will be either dis turbed or, at best, decelerated. In order to correctly formulate a solution to this problem, it is necessary to determine at the previous hierarchical levels the qual ity and quantity of "fragments" involved in the assem bly of the whole, compare the fragments and the result of their assembly, and study their kinetic parameters. These assumptions formed the basis of this work. The apical parts of the roots of Allium cepa were used as a study object, and the replacement of ordinary water with heavy water served as a weak effect of the environ ment.A large number of studies on the investigation of the bases of morphogenesis in the apical part of roots were carried out with different plants, in particular, with the roots of Zea mays, Allium cepa, etc. [2][3][4]. In recent years, apical meristems are considered as areas containing stem cells, whose activity is the key mech anism in the morphogenetic processes in cells [5][6][7].The morphological structure of these cellular zones and the involvement of different protein regulators in cell differentiation in the apical part of the root [7-9], the role of hormonal regulation of morphogenesis [10, etc.], the influence of various physicochemical condi tions on the growth and differentiation processes in apical meristem cells [11][12][13], etc. are currently actively studied today.In previous work, we attempted to use high resolu tion NMR spectroscopy to study metabolites in homogenates of apical cells of onion roots [14]. This paper presents the results of the study of the possibility of using NMR spectroscopy for a comparative study of root apical fragments of onion (homogenate extract) and the apex of intact roots. MATERIALS AND METHODSThe experiments were carried with roots of seedling onion Allium cepa grown in tap non chlorinated water. To prepare an aqueous extract, five to ten roots were minced, ground in a mortar, and placed in heavy water (~0.6 mL). The homogenate was centrifuged, the clear extract was placed in an NMR ampoule, and NMR spectra were recorded. In the second series of experi ments, the roots of onions (15-20 fragments more than 4 cm long) were placed in an NMR ampoule, which was filled with approximately 0.6 ml of Н 2 О or D 2 О. Another series of experiments was performed with onion roots longer than 14-16 cm, which were not cut off from the bulb. The roots were placed in an NMR ampoule so that they reached the bottom of the ampoule and the bulb was fixed over the ampoule, and poured with ~0.6-0.8 ml of Н 2 О (distilled) and 0.1...

show abstract

Analysis of the Metabolites in Apical Area of Allium cepa Roots by High Resolution NMR Spectroscopy Method

Cited by 8 publications

References 18 publications

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Looking at microbial metabolism by high-resolution²H-NMR spectroscopy

From fragments to morphogenesis: NMR spectroscopy of metabolites in the apex of the roots of onion

Contact Info

Product

Resources

About

Analysis of the Metabolites in Apical Area of Allium cepa Roots by High Resolution NMR Spectroscopy Method

Cited by 8 publications

References 18 publications

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Looking at microbial metabolism by high-resolution2H-NMR spectroscopy

From fragments to morphogenesis: NMR spectroscopy of metabolites in the apex of the roots of onion

Contact Info

Product

Resources

About

Looking at microbial metabolism by high-resolution²H-NMR spectroscopy