Analysis of the kinetics of peritoneal adhesion formation in the rat and evaluation of potential antiadhesive agents

Harris, Elizabeth S.; Morgan, Raymond F.; G, Rodeheaver

doi:10.1016/s0039-6060(95)80010-7

Cited by 206 publications

(151 citation statements)

References 20 publications

Supporting

Mentioning

130

Contrasting

Unclassified

Order By: Relevance

“…Anti-adhesion barriers must maintain their position on the injured site for at least this amount of time to be effective for preventing adhesion. [31] Hill-West et al, [32] showed that HA was not effective in the prevention of postsurgical abdominal adhesion; however, their findings were contradicted by the results of other studies. These inconsistent results may be due to the fact that in some cases, the surgical area is greater than the volume of the HA material.…”

Section: Discussionmentioning

confidence: 94%

Anti-adhesive effect of hyaluronate in a rabbit laminectomy model

Chen¹,

Lee²,

Tsai³

et al. 2014

Biomed J

View full text Add to dashboard Cite

P ostoperative fibrosis after spinal surgeries such as discectomy and laminectomy can sometimes cause neurologic side effects due to direct compression of the nerves by the surrounding structures or by the formation of adhesion between tissues, tethering the nerve roots and dural sac. Previous studies have investigated the close Original Article At a Glance Commentary Scientific background of the subjectHyaluronic acid has been widely used in numerous clinical applications. It has been used to treat knee osteoarthritis by injecting it into the joint. It can act as an anti-adhesion barrier in preventing postoperative scar tissue formation in various surgical procedures such as myomectomy and laparotomy. What this study adds to the fieldHyaluronic acid-based gel is effective in decreasing the tenacity of adhesion between the dura and scar and the amount of scar tissues after laminectomy in a rabbit model, which can be considered as a potential anti-adhesion barrier in preventing postoperative adhesion formation.Background: Postlaminectomy dural adhesion is a common cause of recurrent symptoms. Hyaluronic acid-based gel has been reported to reduce the incidence of postoperative adhesion in the peritoneal cavity; however, its effect on preventing postoperative scar formation at laminectomy sites is not yet known. The purpose of this study was to evaluate the anti-adhesive effect of hyaluronic acid-based gelatin after laminectomy, using a rabbit model. Methods:Twelve adult New Zealand rabbits underwent two-level lumbar laminectomy, and were randomly assigned to one of two groups of six rabbits each. The treatment group received hyaluronic acid-based gelatin treatment and the control group was untreated. Rabbits were sacrificed 8 weeks after treatment. Peel-off testing and histological analysis were performed to assess the tenacity and the extent of adhesion formation. Results:No significant difference was observed in the neurologic performance between the two groups. The tenacity in the treatment group was significantly reduced compared to that of the control group (3.17 ± 0.75 vs. 4.33 ± 0.52, respectively; p = 0.016). Histological analysis showed significantly less scar tissue formation in the treatment group, with a larger subarachnoid space and greater distance between the dura and scar tissues. The amount of fibroblast cells also was significantly smaller in the treatment group than in the control group (3078 ± 313.68 vs. 3742 ± 455.65, respectively; p = 0.042). Conclusions: No serious adverse events were reported, and no difference was found in the incidence of complications between the treatment and control groups. The findings suggested that hyaluronic acid-based gelatin may be effective for preventing postlaminectomy dural adhesion in rabbits. (Biomed J 2014;37:218-224)

show abstract

Section: Discussionmentioning

confidence: 94%

Anti-adhesive effect of hyaluronate in a rabbit laminectomy model

Chen¹,

Lee²,

Tsai³

et al. 2014

Biomed J

View full text Add to dashboard Cite

show abstract

“…Evidence suggests that inflammatory response, includ- ing fibroblast activation and deposition at the site of tissue injury, is a pivotal event in the initial stages of adhesion formation (Wang et al, 2010). The injured surfaces are most susceptible to adhesion formation 16 to 36 h after the operation (Ricketts et al, 1999;Harris et al, 1995). NOCC could function as a barrier during this critical period.…”

Section: Discussionmentioning

confidence: 99%

Use of N,O-carboxymethyl chitosan to prevent postsurgical adhesions in a rabbit double uterine horn model: a randomized controlled design

Ren

Zhao

Zhu

2016

Sci. China Life Sci.

View full text Add to dashboard Cite

In previous studies, N,O-carboxymethyl chitosan has been shown to decrease the incidence and intensity of abdominal adhesions. In the present study, adhesions were induced in 220 rabbits using a double uterine horn model. Rabbits were randomized to receive an operation only or an operation+medical chitosan intraperitoneally. Twenty-two rabbits from each group were euthanized at one of five different times (Day 3, 7, 14, 28, or 42), and adhesion formation was given gross and histopathological scores. Reductions were observed in adhesion extent (P=0.0337) and tenacity (P=0.0271) as well as inflammation (P<0.0001) on Day 3 when medical chitosan was applied. Prior to Day 14, fibrosis was less obvious in the medical chitosan group (P<0.0005). The tenacity scores were significantly lower in the medical chitosan group following Day 14 (P<0.05), while the type scores were lower in the medical chitosan group following Day 28 (P<0.03). Thus, medical chitosan decreased both the gross and the histopathological scores of the induced adhesions.

show abstract

“…These cellular mediators are produced by the cellular components of the peritoneal fluid such as the mast cells, the macrophages, the neutrophils and the eosinophils [10,11]. Surgical trauma triggers inflammation process which causes an increase in vascular permeability and release of fibrinrich exudates [12]. If the fibrin is not lysed through the plasminogen-plasmin cascade, adhesions may form through collagen deposition on a framework of proteoglycan and fibronectin [13].…”

Section: Discussionmentioning

confidence: 99%

Mast cells in peritoneal fluid in rats with experimentally induced peritoneal adhesions.

Hermanowicz

Dębek²,

Oksiuta³

et al. 2010

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Mast cells (MC) produce, store and release many biologically active substances, especially inflammatory factors, chemotactic substances for neutrophiles, cytokines and prostaglandins. They play very important role in fibrinosis and they are an important factor in peritoneal adhesions formation and lysis. In this study we tried to evaluate role of mast cells in peritoneal adhesions formation. We estimated number of mast cells in peritoneal fluid in rats with experimentally developed peritoneal adhesions. The number of mast cells per ml was counted in flow cytometry in specimens of peritoneal fluid taken from operated rats. The fluid was taken in standarized conditions the same for each group at the first operation and during reoperation. Peritoneal cavity was washed with 0.9% Saline solution. MC were visualized using indirect immunohistochemical method LSAB with mouse antibody. The animals were divided into 4 groups. 1 st group was control group (n=20) on which the abdomen was opened and closed without any manipulations, and the reoperation was done after 72 hours. The other groups (2, 3, 4; n=20 for each group) were operated and scarification of the partial peritoneum and serosa was performed. The rats were brought back to conscious and then were reoperated respectively after 24, 72 and 168 hours after first surgery. After the laparotomy and damage of the peritoneum we observed formation of the peritoneal adhesions between intestine loops and between intestines and damaged parietal peritoneum. Also the higher number of mast cells was observed in the groups of animals with damaged peritoneum. The highest number of peritoneal adhesions was observed in the group of animals reoperated after 72 hours. After 72 and 168 hours the higher number of MC and neutrophils was observed. The difference was statistically significant. The percentage of mast cells was increasing during the experiment duration. It was different from other cells populations which decreased after 168 hours. The MC and neutrophils were cell population which changed significantly after manipulations in peritoneal cavity. It is very probable that they play an important role in peritoneal adhesions formation.

show abstract

Analysis of the kinetics of peritoneal adhesion formation in the rat and evaluation of potential antiadhesive agents

Cited by 206 publications

References 20 publications

Anti-adhesive effect of hyaluronate in a rabbit laminectomy model

Anti-adhesive effect of hyaluronate in a rabbit laminectomy model

Use of N,O-carboxymethyl chitosan to prevent postsurgical adhesions in a rabbit double uterine horn model: a randomized controlled design

Mast cells in peritoneal fluid in rats with experimentally induced peritoneal adhesions.

Contact Info

Product

Resources

About