Analysis of the Interactions of Migrating Macrophages

Solís-Lemus, José Alonso; Stramer, Brian; Slabaugh, Greg; Reyes-Aldasoro, Constantino Carlos

doi:10.1007/978-3-319-95921-4_25

Cited by 3 publications

(8 citation statements)

References 16 publications

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“…Through the analysis made in this work, the time in clump was found to be helpful when assessing the change of direction in values of TC < 10. The result was not previously found in [18], therefore it fuels the need for a more thorough analysis of the tracks' parameters, attempting to characterize them.…”

Section: Discussionmentioning

confidence: 79%

“…[16] showed an analysis of the shape evolution of cells that do not overlap. Finally, the initial study to understand macrophages' direction changes as a consequence of cell-cell contact was presented in [18]. In this work, macrosight is presented as a complete framework which includes the routines for data handling, segmentation as well as shape and tracks analysis.…”

Section: Discussionmentioning

confidence: 99%

“…This work is an extension to the work shown in [18]. The principal extension made was the presentation of a full framework of routines.…”

Section: Discussionmentioning

confidence: 99%

“…Figure 1 shows a graphical abstract of the main contribution of the direction changing in the algorithm. A preliminary version of this work was presented at the 22nd Medical Image Understanding and Analysis (MIUA) [18]. The algorithms have been extended and several new experiments with new data are presented.…”

Section: Introductionmentioning

confidence: 99%

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Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab®

et al. 2019

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This paper presents a novel software framework, called macrosight, which incorporates routines to detect, track, and analyze the shape and movement of objects, with special emphasis on macrophages. The key feature presented in macrosight consists of an algorithm to assess the changes of direction derived from cell–cell contact, where an interaction is assumed to occur. The main biological motivation is the determination of certain cell interactions influencing cell migration. Thus, the main objective of this work is to provide insights into the notion that interactions between cell structures cause a change in orientation. Macrosight analyzes the change of direction of cells before and after they come in contact with another cell. Interactions are determined when the cells overlap and form clumps of two or more cells. The framework integrates a segmentation technique capable of detecting overlapping cells and a tracking framework into a tool for the analysis of the trajectories of cells before and after they overlap. Preliminary results show promise into the analysis and the hypothesis proposed, and lays the groundwork for further developments. The extensive experimentation and data analysis show, with statistical significance, that under certain conditions, the movement changes before and after an interaction are different from movement in controlled cases.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

“…This work is an extension to the work shown in [18]. The principal extension made was the presentation of a full framework of routines.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab®

et al. 2019

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“…However, few studies have been made on identifying patterns in the migration or providing a biological context to the tracks obtained. In previous work, the analysis of macrophages' movement has been studied in the context of the cell-shape evolution [9], as well as the comparison of movement patterns of interacting cells from non-interacting [10]. In [11], a framework was presented to analyse the tracks of migrating macrophages, analysing the movement related to the interactions.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Interactions Between Control and Mutant Macrophages

Solís-Lemus

Sánchez-Sánchez

Marcotti

et al. 2020

Communications in Computer and Information Science

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This paper presents a preliminary study on macrophages migration in Drosophila embryos, comparing two types of cells. The study is carried out by a framework called macrosight which analyses the movement and interaction of migrating macrophages. The framework incorporates a segmentation and tracking algorithm into analysing motion characteristics of cells after contact. In this particular study, the interactions between cells is characterised in the case of control embryos and Shot3 mutants, where the cells have been altered to suppress a specific protein, looking to understand what drives the movement. Statistical significance between control and mutant cells was found when comparing the direction of motion after contact in specific conditions. Such discoveries provide insights for future developments in combining biological experiments to computational analysis.

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Comparative study of contact repulsion in control and mutant macrophages using a novel interaction detection

Solís-Lemus

Sánchez-Sánchez

Burki

et al. 2020

Preprint

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This paper compares the contact-repulsion movement of mutant and wild-type macrophages using a novel interaction detection mechanism. The migrating macrophages are observed in Drosophila embryos. The study is carried out by a framework called macrosight, which analyses the movement and interaction of migrating macrophages. The framework incorporates a segmentation and tracking algorithm into analysing motion characteristics of cells after contact. In this particular study, the interactions between cells is characterised in the case of control embryos and Shot3 mutants, where the cells have been altered to suppress a specific protein, looking to understand what drives the movement. Statistical significance between control and mutant cells was found when comparing the direction of motion after contact in specific conditions. Such discoveries provide insights for future developments in combining biological experiments to computational analysis. Cell Segmentation,

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Analysis of the Interactions of Migrating Macrophages

Abstract: This is the accepted version of the paper.This version of the publication may differ from the final published version. Permanent repository link

Cited by 3 publications

References 16 publications

Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab®

Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab®

Comparison of Interactions Between Control and Mutant Macrophages

Comparative study of contact repulsion in control and mutant macrophages using a novel interaction detection

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