Analysis of the Interaction Between Plasmodium falciparum-Infected Erythrocytes and Human Endothelial Cells Using a Laminar Flow System, Bioinformatic Tracking and Transcriptome Analysis

Wu, Yifan; Bouws, Philip; Lorenzen, Stephan; Bruchhaus, Iris; Metwally, Nahla Galal

doi:10.1007/978-1-0716-1681-9_11

Cited by 1 publication

(2 citation statements)

References 24 publications

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“…As we published previously [70,71], the following steps were performed. A total of 2x10 5 ECs in one laminar ow slide were seeded 2 days before the assay.…”

Section: Micro Uidic Pump and Shear Stress Applicationmentioning

confidence: 99%

“…iRBCs must be highly synchronized (ring/trophozoite/schizont stage). Gelatine otation [70] is used to select the trophozoite/schizont stages on the day of assay or 1 day before the assay to obtain synchronized ring stages iRBCs. On the day of assay, the ring-stage iRBCs were resuspended in 1 mL serum-free RMPI medium to estimate the cell count.…”

Section: Endothelial Cell Culturementioning

confidence: 99%

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Tissue-specific responses of brain and lung endothelial cell miRNA and mRNA profiles to the ring-stage Plasmodium falciparum-infected red blood cells

Metwally,

Tauler,

Torabi

et al. 2024

Preprint

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MicroRNAs (miRNAs) control 60% of genes expressed in the human body, but their role in malaria pathogenesis is incompletely understood. For the first time, we demonstrate cell type-specific alterations to the miRNA profiles during the early response to malaria infection in brain and lung endothelial cells (ECs). In brain ECs, incubation with Plasmodium falciparum-infected red blood cells in the ring stage (iRBCs) most significantly affected endocytosis-related miRNAs and mRNAs. Contrastingly, in lung ECs, iRBCs altered electron transport chain-related miRNAs and mRNAs. We also present a novel dataset of inherent differences between microRNA profiles in brain and lung ECs and their secreted extracellular vesicles (EVs). We demonstrated that shear stress affected multiple pathways in brain ECs, which were controlled by numerous human miRNAs. Together, these findings demonstrate that host miRNAs respond to parasite exposure; this is accompanied by stimulation of downstream signaling pathways within the ECs. Therefore, we consider miRNAs to be the initial spark (Code of duty) for the early host-parasite interaction events.

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“…As we published previously [70,71], the following steps were performed. A total of 2x10 5 ECs in one laminar ow slide were seeded 2 days before the assay.…”

Section: Micro Uidic Pump and Shear Stress Applicationmentioning

confidence: 99%

Section: Endothelial Cell Culturementioning

confidence: 99%

Tissue-specific responses of brain and lung endothelial cell miRNA and mRNA profiles to the ring-stage Plasmodium falciparum-infected red blood cells

Metwally,

Tauler,

Torabi

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Analysis of the Interaction Between Plasmodium falciparum-Infected Erythrocytes and Human Endothelial Cells Using a Laminar Flow System, Bioinformatic Tracking and Transcriptome Analysis

Cited by 1 publication

References 24 publications

Tissue-specific responses of brain and lung endothelial cell miRNA and mRNA profiles to the ring-stage Plasmodium falciparum-infected red blood cells

Tissue-specific responses of brain and lung endothelial cell miRNA and mRNA profiles to the ring-stage Plasmodium falciparum-infected red blood cells

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