Analysis of the immune response in infections of the goldfish (Carassius auratus L.) with Mycobacterium marinum

Hodgkinson, Jordan W.; Ge, Jun-Qing; Grayfer, Leon; Stafford, James L.; Belosevic, Miodrag

doi:10.1016/j.dci.2012.07.006

Cited by 35 publications

(21 citation statements)

References 50 publications

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“…; Hodgkinson et al . ). In addition, intracellular microbicidal products, such as NOS and other reactive oxygen species, are expected to be elevated.…”

Section: Discussionmentioning

confidence: 97%

“…When compared to the transcriptome of zebrafish experimentally infected with M. marinum, the H. erectus transcriptome contains many homologous putative genes associated with innate immunity (van der Sar et al 2009). The early phase of mycobacteriosis is known to elicit an innate immune response, with upregulation of acute inflammatory cytokines, such as IL-1b, IL-6 and TNF (van der Sar et al 2009;Jeon et al 2011;Hodgkinson et al 2012). In addition, intracellular microbicidal products, such as NOS and other reactive oxygen species, are expected to be elevated.…”

Section: Discussionmentioning

confidence: 99%

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Pathologic features of mycobacteriosis in naturally infected Syngnathidae and novel transcriptome assembly in association with disease

Fogelson

Fast

Leary

et al. 2017

Journal of Fish Diseases

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Syngnathidae (seahorses, seadragons and pipefish) suffer significant losses from non-tuberculous mycobacteria. However, they produce markedly different lesions in response to the disease compared to other teleost species, notably infrequent granuloma formation. This study evaluated 270 syngnathid fish, from which 92 were diagnosed with mycobacteriosis by histopathology, culture or both. Microscopic lesions variably consisted of random foci of coagulative necrosis in multiple organs, containing high numbers of free bacteria and large aggregates or sheets of macrophages with cytoplasm laden with acid-fast bacilli. Mycobacterial associated granulomas were identified in only six seahorses. Five fish had positive cultures with no observed microscopic changes. RNA-seq of the head kidney was performed to investigate the transcriptome of two infected and six non-infected lined seahorses Hippocampus erectus. Assembled and annotated putative transcripts serve to enrich the database for this species, as well as provide baseline data for understanding the pathogenesis of mycobacteriosis in seahorses. Putative components of the innate immune system (IL-1β, IL-6, TNF, NOS, Toll-like receptor 1, MHC Class I, NF-κβ, transforming growth factor beta, MyD88) were identified in the RNA-seq data set. However, a homolog for a key component in the TH1 adaptive immune response, interferon-gamma, was not identified and may underlie the unique pathologic presentation.

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“…; Hodgkinson et al . ). In addition, intracellular microbicidal products, such as NOS and other reactive oxygen species, are expected to be elevated.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Pathologic features of mycobacteriosis in naturally infected Syngnathidae and novel transcriptome assembly in association with disease

Fogelson

Fast

Leary

et al. 2017

Journal of Fish Diseases

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“…In detail, the ESX-5 system of the mycobacteria is responsible for the secretion of various proline-prolineglutamic acid (PPE) and proline-glutamic acid (PE)polymorphic GC-rich repetitive sequence (PGRS) proteins. Animal model studies suggest that such proteins interact with host immune components and possibly subvert critical innate immune pathways, establishing a moderate and persistent infection [29][30][31][32][33]. In vitro observations on infected human macrophages further suggest that these proteins strongly modulate the human macrophage response and actively suppress T-lymphocyte receptor signalingdependent innate immune cytokine secretion, thus allowing bacterial survival [34].…”

Section: Discussionmentioning

confidence: 99%

Aquarium-borne Mycobacterium marinum skin infection. Report of 15 cases and review of the literature

Bonamonte¹,

Vito²,

Vestita³

et al. 2013

European Journal of Dermatology

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Mycobacterium marinum is a non-tuberculous photochromogenic mycobacterium, commonly responsible for fish and amphibious infections world-wide. Contagion in humans typically follows minor hand trauma from aquarium keeping and manifests as a granulomatous infection of the skin. Dissemination is rare and almost exclusive to immunosuppressed hosts. 15 cases of M. marinum fish tank related infection are hereby reported. The site of infection was the upper limbs in all cases. 3 patients presented a single papulo-verrucous lesion, while the remaining 12 showed a sporotrichoid clinical pattern. Diagnosis was reached by history and clinical examination and further supported by one or more of the following criteria: histology, culture, acid fast bacilli identification from histologic specimen and PCR. 2 to 3 months minocycline treatment showed efficacy in 13 individuals, another case was treated with rifampicin-isoniazid association, yet another showed spontaneous regression over a 3 month period.

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“…The goldfish has historically served as an important model organism in physiological, behavioral, immunological and molecular evolutional studies (Ge et al, 1993; Hodgkinson et al, 2012; Huntingford, 2012). The goldfish can regenerate various organs and tissues including scale, fins, intramembranous bone, hair cells, optic nerve and spinal cord (Birnie, 1947; Caskey and O'Brien, 1948; Bernstein, 1964; Matsukawa et al, 2004; Smith et al, 2006; Thamamongood et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Cardiac repair and regenerative potential in the goldfish (Carassius auratus) heart

Grivas

Haag

Johnson

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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The remarkable ability of the heart to regenerate has been demonstrated in the zebrafish and giant danio, two fish members of the cyprinid family. Here we use light and electron microscopy to examine the repair response in the heart of another cyprinid, the goldfish (Carassius auretus), following cautery injury to a small portion of its ventricular myocardium. We observed a robust inflammatory response in the first two weeks consisting primarily of infiltrating macrophages, heterophils, and melanomacrophages. These inflammatory cells were identified in the lumen of the spongy heart, within the site of the wound, and attached to endocardial cells adjacent to the site of injury. Marked accumulation of collagen fibers and increased connective tissue were also observed during the first and second week in a transition zone between healthy and injured myocardium as well as in adjacent sub-epicardial regions. The accumulation of collagen and connective tissue however did not persist. The presence of capillaries was also noted in the injured area during repair. The replacement of the cauterized region of the ventricle by myocardial tissue was achieved by 6 weeks. The presence of ethynyl deoxyuridine-positive cardiac myocytes and partially differentiated cardiac myocytes during repair suggest effective cardiac myocyte driven regeneration mechanisms also operate in the injured goldfish heart, and are similar to those observed in zebrafish and giant danio. Our data suggest the ability for cardiac regeneration may be widely conserved among cyprinids.

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Analysis of the immune response in infections of the goldfish (Carassius auratus L.) with Mycobacterium marinum

Cited by 35 publications

References 50 publications

Pathologic features of mycobacteriosis in naturally infected Syngnathidae and novel transcriptome assembly in association with disease

Pathologic features of mycobacteriosis in naturally infected Syngnathidae and novel transcriptome assembly in association with disease

Aquarium-borne Mycobacterium marinum skin infection. Report of 15 cases and review of the literature

Cardiac repair and regenerative potential in the goldfish (Carassius auratus) heart

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