Analysis of the human antibody response to thrombospondin-related anonymous protein of Plasmodium falciparum

Scarselli, Elisa; Tolle, Ralf; Koïta, Ousmane; Diallo, Mahamadou; Müller, Hans‐Michael; Früh, Klaus; Doumbo, Ogobara K.; Crisanti, Andrea; Bujard, Hermann

doi:10.1128/iai.61.8.3490-3495.1993

Cited by 38 publications

(13 citation statements)

References 29 publications

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“…However, the difference between dry and wet seasons is markedly greater in the < 4-year-age class than among the 5-9-year-olds and the 10-19-year-age class. Similar patterns have been reported recently in region of West Africa where malaria is hyperendemic (Scarselli et al 1993). This implies that recovery rates are higher among those under 4 years of age, as the effect of a change in transmission will manifest itself as a change in parasite rates only if the duration of parasitaemia is short.…”

Section: Discussionsupporting

confidence: 86%

A theoretical framework for the immunoepidemiology ofPlasmodium falciparummalaria

Gupta

Day

1994

Parasite Immunology

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Molecular genetic analyses of P. falciparum have led to the cloning and sequencing of a number of antigens that are potential candidates for vaccination against malaria. Seroepidemiological studies in endemic areas have attempted to assess the relative importance of these antigens in protection against malaria. In this paper, we attempt to evaluate the relative contributions of conserved and strain-specific immune responses by modelling their influence of age-specific patterns of infection and disease. The modelling exercises in this paper clearly demonstrate that the observed patterns of age-prevalence are best explained by proposing that the accumulation to a threshold of an immune response against a conserved determinant is required for protection against infection, while 'anti-disease' immunity develops more linearly with exposure. This is compatible with the conjecture that the parasite population is structured into several independently transmitted strains, that each confers some degree of 'anti-disease' immunity, but does not protect against further infection by the same strain. Within this framework, the average duration of parasitaemia increases with age, as previously encountered strains endure for longer periods at a subclinical level. Indirect evidence for the increase in duration of parasitaemia with age may be obtained from a comparison of age-prevalence curves between dry and rainy seasons. By using mathematical methods to structure epidemiological and immunological information, we provide a coherent theoretical framework for the dissection of the important components of naturally acquired immunity to malaria.

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Section: Discussionsupporting

confidence: 86%

A theoretical framework for the immunoepidemiology ofPlasmodium falciparummalaria

Gupta

Day

1994

Parasite Immunology

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“…Previous work has shown that volunteers immunized with irradiated sporozoites (27) and West African adults and children (29) have antibodies to recombinant PfSSP2/TRAP. We found that residents of malaria-endemic areas had antibodies to the (DRYIPYSP) 3 and (CHPSDGKCN) 2 epitopes.…”

Section: Resultsmentioning

confidence: 99%

Development of two monoclonal antibodies against Plasmodium falciparum sporozoite surface protein 2 and mapping of B-cell epitopes

et al. 1997

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The Plasmodium yoelii sporozoite surface protein 2 (PySSP2) is the target of protective cellular immunity. Cytotoxic T cells specific for the Plasmodium falciparum analog PfSSP2, also known as thrombospondin-related anonymous protein (TRAP), are induced in human volunteers immunized with irradiated sporozoites. PfSSP2 is an important candidate antigen for a multicomponent malaria vaccine. We generated and characterized three monoclonal antibodies (MAbs) specific for PfSSP2/TRAP. The MAbs PfSSP2.1 (immunoglobulin G1 [IgG1]), PfSSP2.2 (IgG2a), and PfSSP2.3 (IgM) were species specific and identified three distinct B-cell epitopes containing sequences DRYI, CHPSDGKC, and TRPHGR, respectively. PfSSP2.1 partially inhibited P. falciparum liver-stage parasite development in human hepatocyte cultures (42 and 86% in two experiments at 100 g/ml). Mice immunized with vaccinia virus expressing full-length PfSSP2 protein produced antibodies to (DRYIPYSP) 3 , and humans living in malaria-endemic areas (Indonesia and Kenya), who have lifelong exposure and partial clinical immunity to malaria, had antibodies to both (DRYIPYSP) 3 and (CHPSDGKCN) 2. Mice immunized with multiple antigen peptides MAP4 (DRYIPYSP) 3 P2P30 and MAP4 (CHPSDGKCN) 3 P2P30 in TiterMax developed antibodies to sporozoites that partially inhibited sporozoite invasion of human hepatoma cells (39 to 71% at a serum dilution of 1:50 in three different experiments). The modest inhibitory activities of the MAbs and the polyclonal antibodies to PfSSP2/TRAP epitopes do not suggest that a single-component vaccine designed to induce antibodies against PfSSP2/TRAP will be protective. Nonetheless, the MAbs directed against PfSSP2, and the peptides recognized by these MAbs, will be essential reagents in the development of PfSSP2/TRAP as a component of a multivalent P. falciparum human malaria vaccine.

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“…Moreover, two extracellular portions of malarial TRAP, the von Willebrand A‐domain and the thrombospondin repeats located in domains II and IV, respectively, are crucial for initial host cell adherence and stabilisation of adhesion/deadhesion during gliding mobility of sporozoites . Importantly, naturally acquired antibodies against P. falciparum TRAP (PfTRAP) exhibited less parasite density among individuals in malaria hyperendemic areas, supporting the role of this molecule in eliciting protective immunity against malaria parasite . Furthermore, immunisation of Aotus monkeys with P. vivax TRAP (PvTRAP)‐derived peptide has conferred partial protection upon challenge infection with a heterologous parasite strain .…”

Section: Introductionmentioning

confidence: 96%

Naturally acquired IgG antibodies to thrombospondin‐related anonymous protein of Plasmodium vivax (PvTRAP) in Thailand predominantly elicit immunological cross‐reactivity

Kosuwin

Feng

Makiuchi

et al. 2018

Tropical Med Int Health

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Analysis of the human antibody response to thrombospondin-related anonymous protein of Plasmodium falciparum

Cited by 38 publications

References 29 publications

A theoretical framework for the immunoepidemiology ofPlasmodium falciparummalaria

A theoretical framework for the immunoepidemiology ofPlasmodium falciparummalaria

Development of two monoclonal antibodies against Plasmodium falciparum sporozoite surface protein 2 and mapping of B-cell epitopes

Naturally acquired IgG antibodies to thrombospondin‐related anonymous protein of Plasmodium vivax (PvTRAP) in Thailand predominantly elicit immunological cross‐reactivity

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