Analysis of the Growth Cycle of Mycoplasma orale by Synchronized Division and by Ultraviolet Irradiation

Furness, G.

doi:10.1093/infdis/118.4.436

Cited by 24 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, if 1 in 1000 virus particles is fully shielded, the effect will be tailing above 3 log reductions, while fully dispersed virus will demonstrate intrinsic kinetics through higher inactivation levels. These effects were recognized and addressed by Furness [37] and by Das [30] in studying the UV inactivation kinetics of mycoplasma, in which they used sonication and filtration to ensure single-cell suspensions. As Furness [37] (2)…”

Section: Other Errorsmentioning

confidence: 99%

See 1 more Smart Citation

Evaluating ultraviolet sensitivity of adventitious agents in biopharmaceutical manufacturing

Meunier

Sasges

Aucoin

2017

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

Incidents of contamination in biopharmaceutical production have highlighted the need to apply alternative or supplementary disinfection techniques. Ultraviolet (UV) irradiation is a well-established method for inactivating a broad range of microorganisms, and is therefore a good candidate as an orthogonal technique for disinfection. To apply UV as a safeguard against adventitious agents, the UV sensitivity of these target agents must be known so that the appropriate dose of UV may be applied to achieve the desired level of inactivation. This document compiles and reviews experimentally derived 254 nm sensitivities of organisms relevant to biopharmaceutical production. In general, different researchers have found similar sensitivity values despite a lack of uniformity in experimental design or standardized quantification techniques. Still, the lack of consistent methodologies has led to suspicious UV susceptibilities in certain instances, justifying the need to create a robust collection of sensitivity values that can be used in the design and sizing of UV systems for the inactivation of adventitious agents.

show abstract

Section: Other Errorsmentioning

confidence: 99%

“…These effects were recognized and addressed by Furness [37] and by Das [30] in studying the UV inactivation kinetics of mycoplasma, in which they used sonication and filtration to ensure single-cell suspensions. As Furness [37] (2)…”

Section: Other Errorsmentioning

confidence: 99%

Evaluating ultraviolet sensitivity of adventitious agents in biopharmaceutical manufacturing

Meunier

Sasges

Aucoin

2017

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

show abstract

“…1. It has been reported that the UV sensitivity of mycoplasmas is significantly affected by their growth stage (4,13,16). The data described here, therefore, are not sufficient for accuratey comparisons of UV sensitivity among five strains of human mycoplasma Caffeine has been widely used as an effective repair inhibitor since Metzger (23) found with E. coli that caffeine blocks dark reactivation of UV-irradiated cells.…”

Section: Recovery Of the Colony-forming Ability Of Uv-irradiated Cellmentioning

confidence: 80%

“…These results suggest the importance of repair systems for maintaining genetic continuity in the simplest organism, as emphasized by Smith and Hanawalt (34). Studies on UV inactivation of human mycoplasmas have been extensively reported by Furness and his coworkers and their results show that single cells prepared by sonication and/or filtration through a membrane filter are inactivated exponentially by UV light (13,14,16,17). However, there is no information on the existence of a repair system for UV-induced damage in DNA of human mycoplasmas.…”

mentioning

confidence: 99%

UV Survival of Human Mycoplasmas: Evidence of Dark Reactivation in Mycoplasma buccale

Aoki

Ito

Watanabe

1979

Microbiology and Immunology

View full text Add to dashboard Cite

The inactivation by ultraviolet (UV) light irradiation of mycoplasma cells of five human strains was monitored by investigating the colony-forming ability. The survival curves of five strains tested indicated that the cells of Mycoplasma buccale only are single and homogenously susceptible to UV light. The effect of the repair inhibitor, caffeine, on the colony-forming ability of UV-irradiated cells was investigated with Al. buccale because of its homogenous susceptibility to UV light. The colony formation of irradiated cells was markedly depressed by post-irradiation treatment with caffeine at concentrations that had little or no effect on the colony formation of unirradiated cells. The colony-forming units (CFU) of UV-irradiated cells which were kept in broth without caffeine in the dark increased without a lag as the time in the dark increased. The colony-forming ability of the irradiated cells completely recovered after 3 hr in the dark. However, when irradiated cells were kept in the presence of caffeine, no increase in their CFU was observed. The mode of action of caffeine on UV-irradiated cells closely resembles that described for other organisms which possess dark reactivation systems for UV-induced damage in deoxyribonucleic acid (DNA). Thus, the results obtained provide evidence for the existence of a dark repair function in M. buccale.

show abstract

“…Greater knowledge of the production of elementary bodies is therefore urgently required, but little work has been done on this. Morowitz & Maniloff (1966) and Furness (1968) have studied the growth cycle of Mycoplasma gallisepticum A5969 and M . orale respectively.…”

Section: )mentioning

confidence: 99%

Electron Microscopic Studies of Mycoplasma pulmonis (Negroni Strain)

Tanaka

Woods

1970

Journal of General Microbiology

View full text Add to dashboard Cite

Analysis of the Growth Cycle of Mycoplasma orale by Synchronized Division and by Ultraviolet Irradiation

Cited by 24 publications

References 0 publications

Evaluating ultraviolet sensitivity of adventitious agents in biopharmaceutical manufacturing

Evaluating ultraviolet sensitivity of adventitious agents in biopharmaceutical manufacturing

UV Survival of Human Mycoplasmas: Evidence of Dark Reactivation in Mycoplasma buccale

Electron Microscopic Studies of Mycoplasma pulmonis (Negroni Strain)

Contact Info

Product

Resources

About