Analysis of the grapevine moth Lobesia botrana antennal transcriptome and expression of odorant-binding and chemosensory proteins

Rojas, Valentina; Jiménez, Héctor Miguel Aviña; Palma-Millanao, Rubén; González-González, Angélica; Machuca, Juan; Godoy, Ricardo Chirino; Ceballos, Ricardo; Mutis, Ana; Venthur, Herbert

doi:10.1016/j.cbd.2018.04.003

Cited by 24 publications

(23 citation statements)

References 48 publications

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“…Contrary to our hypothesis that LbotPBP1 is a specific carrier for the main sex pheromone component, ( E , Z )‐7,9‐dodecadienyl acetate, our structural investigations, and fluorescence binding assays indicate that the compound is not exclusively bound by the protein. It is probable that other PBPs and GOBPs, as recently confirmed by antennal transcriptome studies (Rojas et al, ), can have stronger binding affinities to ( E , Z )‐7,9‐dodecadienyl acetate and can preferably transport this chemical as well as other OBPs transport host plant volatiles. On the other hand, the limited selectivity of PBPs can be debated with respect to distinguishing between double bonds and cis/transpositions.…”

Section: Discussionmentioning

confidence: 83%

“…The above approach has supported L. botrana as a good target of research at not only the behavioral level but also at the molecular level. Thus, recent studies have reported the presence of a PBP in the antennae of L. botrana (LbotPBP1; Mutis et al, ), and significant expression in male antennae was further corroborated by antennal transcriptome and qRT‐PCR experiments (Rojas et al, ).…”

Section: Introductionmentioning

confidence: 85%

“…This study evaluated a PBP of L. botrana , which is a grapevine pest distributed worldwide. From three PBP ‐related transcripts, we selected one (i.e., LbotPBP1) that has shown male‐biased expression, which could shed light on its functional role in the olfactory system of L. botrana (Rojas et al, ). Likewise, 11 sex pheromone components plus 6 host plant volatiles were selected for binding assays and structural analyses.…”

Section: Discussionmentioning

confidence: 99%

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Structural investigation of selective binding dynamics for the pheromone‐binding protein 1 of the grapevine moth, Lobesia botrana

Venthur

Machuca

Godoy

et al. 2019

Arch Insect Biochem Physiol

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The European grapevine moth, Lobesia botrana (Denis & Schiffermüller), is a serious pest in vineyards in North and South America. Mating disruption techniques have been used to control and monitor L. botrana on the basis of its sexual communication. This needs a well-tuned olfactory system, in which it is believed that pheromone-binding proteins (PBPs) are key players that transport pheromones in the antennae of moths. In this study, the selectivity of a PBP, named as LbotPBP1, was tested by fluorescence binding assays against 11 sex pheromone components and 6 host plant volatiles. In addition, its binding mechanism was predicted on the basis of Arch. Insect Biochem. Physiol. 2019;101:e21557.wileyonlinelibrary.com/journal/arch *These authors contributed equally to this work. structural analyses by molecular docking and complex and steered molecular dynamics (SMD). Our results indicate that LbotPBP1 binds selectively to sex pheromone components over certain host plant volatiles, according to both in vitro and in silico tests. Thus, chain length (14 carbon atoms) and functional groups (i.e., alcohol and ester) appear to be key features for stable binding. Likewise, residues such as Phe12, Phe36, and Phe118 could participate in unspecific binding processes, whilst Ser9, Ser56, and Trp114 could participate in the specific recognition and stabilization of sex pheromones instead of host plant volatiles. Moreover, our SMD approach supported 11-dodecenyl acetate as the best ligand for LbotPBP1. Overall, the dynamics simulations, contact frequency analysis and SMD shed light on the binding mechanism of LbotPBP1 and could overcome the imprecision of molecular docking, supporting the in vitro binding assays. Finally, the role of LbotPBP1 in the chemical ecology of L. botrana is discussed. K E Y W O R D S molecular modeling, odorant-binding protein, olfactory system, Tortricidae

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

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Structural investigation of selective binding dynamics for the pheromone‐binding protein 1 of the grapevine moth, Lobesia botrana

Venthur

Machuca

Godoy

et al. 2019

Arch Insect Biochem Physiol

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“…An analysis of the expression patterns of CSPs, indicated that exploration of the functions of CobdCSP14 and CobdCSP3 in antenna and other tissues will be important. Some CSPs were significantly expressed in antennae, including most of the CSPs of Lobesia botrana (Rojas et al, 2018) and MmedCSP2 and MmedCSP3 of Microplitis mediator (Peng et al, 2017). MmedCSP3 can bind insect odors and plant volatiles, as well as pheromone components of Noctuidae, Z11-16:Ald, Z11-16: OH and E11-14:Ac (Peng et al, 2017), illustrating the binding ability and functions of CSPs in olfactory recognition.…”

Section: Discussionmentioning

confidence: 92%

“…In terms of the number of olfactory proteins, C. obducta had similar amount OBPs compared with the whole body transcriptome of Oedaleus infernalis . Compared with the insect antenna transcriptome (Chang et al, 2017;Nie et al, 2017;Rojas et al, 2018;Sheng et al, 2017;Wu et al, 2017;Yang et al, 2017Yang et al, , 2018) (File S8), C. obducta had an intermediate number of OBPs. The number of olfactory proteins identified in the whole body transcriptome of C. obducta is smaller than that inmost other species based on analyses of insect antennal genes.…”

Section: Discussionmentioning

confidence: 99%

The whole body transcriptome of Coleophora obducta reveals important olfactory proteins

Wang

Tao

et al. 2020

PeerJ

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Background The tiny casebearer moth Coleophora obducta, an important defoliator of Larix spp., is a major threat to ecological security in north China. Studies have shown that C. obducta is strongly specific to host plants; it is unable complete its life cycle without Larix spp. The sex pheromones of C. obducta Z5-10:OH have been elucidated; and eight types of antennae sensilla, have been detected, indicating that an exploration of its olfactory proteins is necessary, due to the general lack of information on this topic. Methods We investigated the whole body transcriptome of C. obducta, performed a phylogenetic analysis of its olfactory proteins and produced expression profiles of three pheromone-binding proteins (CobdPBPs) by qRT–PCR. Results We identified 16 odorant binding proteins, 14 chemosensory proteins, three sensory neuron membrane proteins, six odorant degrading enzymes, five antennal esterases, 13 odorant receptors, seven ionotropic receptors and 10 gustatory receptors, including three PBPs and one odorant co-receptor. Additionally, three putative pheromone receptors, two bitter gustatory receptors and five functional ionotropic receptors were found by phylogenetic analysis. The expression profiles of three PBPs in males and females showed that all of them exhibited male-specific expression and two were expressed at significantly higher levels in males. These data provide a molecular foundation from which to explore the olfactory recognition process and may be useful in the development of a new integrated pest management strategy targeting olfactory recognition of C. obducta.

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Identification of the differences in olfactory system between male and female oriental tobacco budworm Helicoverpa assulta

Miao

et al. 2021

Arch Insect Biochem Physiol

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The olfactory system of insects facilitates their search for host and mates, hence it plays an essential role for insect survival and reproduction. Insects recognize odor substances through olfactory neurons and olfactory genes.Previous studies showed that there are significant sexspecific differences in how insects identify odorant substances, especially sex pheromones. However, whether the sex-specific recognition of odorant substances is caused by differences in the expression of olfaction-related genes between males and females remains unclear. To clarify this

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Analysis of the grapevine moth Lobesia botrana antennal transcriptome and expression of odorant-binding and chemosensory proteins

Cited by 24 publications

References 48 publications

Structural investigation of selective binding dynamics for the pheromone‐binding protein 1 of the grapevine moth, Lobesia botrana

Structural investigation of selective binding dynamics for the pheromone‐binding protein 1 of the grapevine moth, Lobesia botrana

The whole body transcriptome of Coleophora obducta reveals important olfactory proteins

Identification of the differences in olfactory system between male and female oriental tobacco budworm Helicoverpa assulta

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