Analysis of the gibberellin-responsive promoter of a cathepsin B-like gene from wheat

Cejudo, Francisco Javier; Ghose, T. K.; Stabel, Priska; Baulcombe, David C.

doi:10.1007/bf00027156

Cited by 34 publications

(38 citation statements)

References 30 publications

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“…The promoters of these genes do contain Myb-binding consensus motifs (Fig. 4), and in the case of cathepsin-B deletion of a 26-base domain containing this motif reduces the GA-regulated activity to as little as 26 % of full-length promoter activity (Cejudo et al, 1992). These results support the idea that the GAMyb is a central controller of the GA response in the aleurone.…”

Section: Transcriptional Regulationsupporting

confidence: 63%

Gibberellins: regulating genes and germination

Ritchie

Gilroy

1998

New Phytologist

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The range of processes regulated by gibberellins (GAs) covers all aspects of the life history of the plant from seed germination to vegetative growth and flowering. In seeds there has been an intensive search, using the techniques of both biochemistry and cell biology, for the regulatory molecules linking GA perception to gene regulation and the events of germination. Although a GA receptor has yet to be identified, the site of perception has been localized to the plasma membrane. Calmodulin, Ca2+ and cGMP have also been identified as elements of the GA signal transduction pathway. These regulators parallel many of the signalling elements identified in the transduction of other signals such as phytochrome and ABA. Studies of GA‐regulated gene expression, principally of the α‐amylases of cereal aleurone, have identified core GA‐responsive promoter elements, such as the gibberellin response element (GARE), box‐1 and pyrimidine boxes, as well as elements that may lend specificity to GA‐regulated expression, such as the Opaque‐2‐similar element (O2S), and TRE and CRE motifs. One of the most striking features of all of these studies of the molecular basis of GA action is the interaction of GA‐dependent regulatory elements with those of other factors such as ABA. GA‐response elements also appear to be conserved between disparate GA‐response systems. For example, Myb transcription factors appear to regulate a multitude of GA‐induced genes in cereal aleurone as well as to alter GA responses when expressed in Arabidopsis. Thus the study of GA signal transduction and response systems is highlighting the conservation of regulatory elements used by plants. These common factors, used by distinct signal transduction systems, provide a molecular basis for the integration of the GA signal with other growth regulators that is the hallmark of plant growth and development.

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Section: Transcriptional Regulationsupporting

confidence: 63%

Gibberellins: regulating genes and germination

Ritchie

Gilroy

1998

New Phytologist

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“…Members of this gene family display complex upstream elements responsive to gibberellin and its antagonist abscisic acid which control gene expression in aleurone layers. In addition, dramatic GA-mediated accumulation of the mRNAs for specific thiolproteinases [2,29] and carboxypeptidases [28] could be shown in the aleurone layer of germinating seeds. Also, phytase is likely to be encoded by a GA-regulated gene in barley [ 5].…”

Section: Introductionmentioning

confidence: 99%

GASA, a gibberellin-regulated gene family from Arabidopsis thaliana related to the tomato GAST1 gene

1995

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A multiple gene family of at least four members, related to a GA-stimulated transcript (GAST1) from tomato, was characterized in Arabidopsis thaliana by analysing four related cDNAs, named GASA1 to GASA4. The corresponding peptides display comparable structural features: (1) a putative signal peptide of 18 to 23 residues; (2) a highly divergent hydrophilic region of about 22 amino acids; (3) a conservative 60 amino acid C-terminal domain containing 12 cysteines. This organization has also bean shown in two related peptides from tomato, GAST1 found in shoots and RSI-1 found in early lateral roots. Southern blot hybridization patterns showed single-copy genes for all four members of the GASA family. Accumulation of the various transcripts, monitored by northern blot hybridization, indicated that the various genes are expressed differentially in plant organs: Specific mRNAs were mostly detected in flower buds and immature siliques in the case of GASA1, in siliques and dry seeds in the case of GASA2 and 3, and in growing roots and flower buds in the case of GASA4. At least two of the GASA genes are activated in GA-deficient mutant ga5, as early as 4 to 8 h after spraying with 50 microM GA3. The complex patterns of expression and regulation of the various genes suggest that the related peptides are involved in a developmental regulation process in Arabidopsis.

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“…Genes encoding s-amylase [73 ], a number of proteases [19,85,153,159], (1-3,1-4)-fl-glucanase [163] and BEG1 (globulin-l) [46] are upregulated by GA and, in all cases, this effect is repressed by ABA. The expression of genes encoding alcohol dehydrogenase and an s-amylase/ protease inhibitor is down regulated by GA [73 ].…”

Section: Ga Regulation Of Gene Expression In Aleuronementioning

confidence: 99%