Analysis of the Genomes of Mink Cell Focus-inducing Murine Type-C Viruses: A Progress Report

Lung, Maria Li; Hering, Christian; Hartley, Janet W.; Rowe, Wallace P.; Hopkins, Nancy

doi:10.1101/sqb.1980.044.01.138

Cited by 47 publications

(69 citation statements)

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“…1). An analogous region of the murine leukemia virus genome may be important in distinguishing leukemogenic from nonleukemogenic recombinants of AKR virus (38)(39)(40)(41) …”

Section: Resultsmentioning

confidence: 99%

At least two regions of the viral genome determine the oncogenic potential of avian leukosis viruses.

Robinson¹,

Blais²,

Tsichlis³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

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Recombinants of oncogenic and nononcogenic avian leukosis viruses were tested for their oncogenic potential in chickens. The results indicate that at least two regions of the viral genome determine the oncogenic potential of these viruses. The first region contains sequences that control viral mRNA synthesis. These sequences determine the potential of a virus to induce a low incidence oflymphomas, carcinomas, chondrosarcomas, fibrosarcomas, and osteopetrosis. The second region lies outside the sequences that control viral mRNA synthesis. These sequences determine the ability of a virus to induce a high incidence of lymphomas or osteopetrosis.

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“…1). An analogous region of the murine leukemia virus genome may be important in distinguishing leukemogenic from nonleukemogenic recombinants of AKR virus (38)(39)(40)(41) …”

Section: Resultsmentioning

confidence: 99%

At least two regions of the viral genome determine the oncogenic potential of avian leukosis viruses.

Robinson¹,

Blais²,

Tsichlis³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

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“…and amphotropic (Ampho.) viruses, and 10-12 ,gg of each for MCF viruses (21,28)] were electrophoresed in horizontal 0.7% agarose gels (35-V; 16 hr), transferred to nitrocellulose filters (15), and hybridized with a 32P-labeled nick-translated (29) 5' viral DNA probe (although it contains 0.1 kbp of LTR sequence, the gel-purified 4.6-kbp Sma I fragment in Fig. 1 does not detect LTR sequences in the DNAs under these conditions of exposure) in top part of A or the ecotropic-specific probe (the cloned 0.4-kbp Sma I fragment in Fig.…”

Section: Methodsmentioning

confidence: 99%

Structure of endogenous murine leukemia virus DNA in mouse genomes.

Chattopadhyay¹,

Lander²,

Rands³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

189

124

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“…Ecotropic and xenotropic mouse leukaemia viruses can also be distinguished from each other by 0022-1317/84/0000-6007 $02.00© 1984 SGM structural and antigenic differences in their major envelope glycoproteins (Famulari & Jalalian, 1979;. Dualtropic viruses appear to arise as a result of genetic recombination between endogenous ecotropic and non-endogenous xenotropic leukaemia viruses Lung et al, 1980), a recombination which gives rise to viruses displaying enhanced leukaemogenicity Rowe et al, 1980) and expressing novel envelope glycoprotein with unique structural and antigenic properties (Elder et al, 1977;Niman & Eider, 1980;Walff et al, 1982;O'Donnell & Nowinski, 1980;Schultz et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Structural Differences in Envelope Glycoproteins Associated with Rat Leukaemia Virus Produced by Novikoff Hepatocellular Carcinoma and Spontaneously Transformed Wistar Rat Embryo Cells

Hixson

Allison

McEntire

et al. 1984

Journal of General Virology

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SUMMARYImmunochemical and immunocytochemical techniques have been used to characterize viral glycoproteins and endogenous rat leukaemia viruses (RaLV) produced both by Novikoff hepatocellular carcinoma cells and spontaneously transformed Wistar rat embryo cells (WRC). Results from immunocytochemical analysis demonstrated that RaLV produced by Novikoff and WRC cells could be distinguished by their unique patterns of reactivity with xenoantisera raised against virus particles or viral glycoproteins. This differential labelling was unexpected since all the antisera tested had been shown by immunoprecipitation and immunodepletion analysis to be reactive with viral glycoproteins expressed on the cell surface. Since no significant differences in cell surface-associated viral glycoproteins and those shed from the cell surface were detected by pulse iodination analysis, it was concluded that the apparent discrepancy between immunoferritin labelling and immunoprecipitation analysis resulted from differences in antigen accessibility on intact virions caused by structural differences in the viral glycoproteins expressed on Novikoff and WRC cells. This conclusion was supported by results from ferritin-lectin labelling, affinity chromatography and neuraminidase digestion studies which demonstrated differences in the saccharide moieties on both virion and cell surface-associated viral glycoproteins. Further evidence of structural differences was provided by limited digestion with trypsin and V8 protease of the M~ 64 000 (Nov gp64) and Mr 68 000 (W RC gp68) viral glycoproteins immunoprecipitated from Novikoff and WRC cells, respectively, with either monospecific anti-Rauscher murine leukaemia virus anti-gpT0 serum or monospecific antiserum against Nov gp64 (anti-gp64). Results from digestion studies showed that all the major cleavage fragments from WRC gp68 were of higher molecular weight than their Nov gp64-derived counterparts. Evidence that Nov gp64 and WRC gp68 both share structural homology with other murine viral gp70s was suggested by results from immunoprecipitation analysis with anti-gp70 and anti-gp64 sera under reducing and non-reducing conditions which demonstrated the presence of an interchain disulphide bond in both glycoproteins and showed that at least some of these molecules exist on the cell surface as disulphide-linked heterodimers of Mr 78000 and 82000.

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Analysis of the Genomes of Mink Cell Focus-inducing Murine Type-C Viruses: A Progress Report

Cited by 47 publications

References 0 publications

At least two regions of the viral genome determine the oncogenic potential of avian leukosis viruses.

At least two regions of the viral genome determine the oncogenic potential of avian leukosis viruses.

Structure of endogenous murine leukemia virus DNA in mouse genomes.

Structural Differences in Envelope Glycoproteins Associated with Rat Leukaemia Virus Produced by Novikoff Hepatocellular Carcinoma and Spontaneously Transformed Wistar Rat Embryo Cells

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