Analysis of the Fungal Community in Apple Replanted Soil Around Bohai Gulf

Wang, Gongshuai; Yin, Chengmiao; Pan, Fengbing; Wang, Xiaobao; Li, Xiang; Wang, Yanfang; Wang, Jinzheng; Tian, Changping; Chen, Jie; Mao, Zhiquan

doi:10.1016/j.hpj.2018.05.003

Cited by 73 publications

(59 citation statements)

References 26 publications

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“…So for, F. moniliforme also have been shown to be pathogenic on forest nurseries in Spain [15], Sorghum in India, Maize in Iowa State [16], Scots pine seedlings in Palencia [17], Peach in California [18] and apple in China [19]. F. moniliforme (F. verticillioides), F. oxysporum, and F. proliferatum isolated from replanted apple soil near the Bohai Bay region in China were highly pathogenic to seedlings of Malus hupehensis in our laboratory research cooperation studies [20,21]. Phloridzin is a characteristic dihydrochalcone of the phenolic compounds produced by apple seedlings [11,[22][23][24], and phloridzin in the root exudates of Malus x domestica Borkh.…”

Section: Introductionmentioning

confidence: 56%

ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers

et al. 2021

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Background Apple replant disease (ARD) has been reported from all major fruit-growing regions of the world, and is often caused by biotic factors (pathogen fungi) and abiotic factors (phenolic compounds). In order to clarify the proteomic differences of Fusarium moniliforme under the action of phloridzin, and to explore the potential mechanism of F. moniliforme as the pathogen of ARD, the role of Fusarium spp. in ARD was further clarified. Methods In this paper, the quantitative proteomics method iTRAQ analysis technology was used to analyze the proteomic differences of F. moniliforme before and after phloridzin treatment. The differentially expressed protein was validated by qRT-PCR analysis. Results A total of 4535 proteins were detected, and 293 proteins were found with more than 1.2 times (P< 0.05) differences. In-depth data analysis revealed that 59 proteins were found with more than 1.5 times (P< 0.05) differences, and most proteins were consistent with the result of qRT-PCR. Differentially expressed proteins were influenced a variety of cellular processes, particularly metabolic processes. Among these metabolic pathways, a total of 8 significantly enriched KEGG pathways were identified with at least 2 affiliated proteins with different abundance in conidia and mycelium. Functional pathway analysis indicated that up-regulated proteins were mainly distributed in amino sugar, nucleotide sugar metabolism, glycolysis/ gluconeogenesis and phagosome pathways. Conclusions This study is the first to perform quantitative proteomic investigation by iTRAQ labeling and LC-MS/MS to identify differentially expressed proteins in F. moniliforme under phloridzin conditions. The results confirmed that F. moniliforme presented a unique protein profile that indicated the adaptive mechanisms of this species to phloridzin environments. The results deepened our understanding of the proteome in F. moniliforme in response to phloridzin inducers and provide a basis for further exploration for improving the efficiency of the fungi as biocontrol agents to control ARD.

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Section: Introductionmentioning

confidence: 56%

ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers

et al. 2021

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“…have been shown to be pathogenic (Celar, 2000;Dinget al, 2017;Duan et al, 2017;Hibar et al, 2006;Manici et al, 2003;Ramamoorthy et al, 2002;Steinkellner et al, 2008;Tewoldemedhin et al, 2011b;Yan et al, 2018;Zhu et al, 2016). And F. moniliforme have also been shown to be pathogenic on forest nurseries in Spain (Pinto et al, 2006), Sorghum in India, Maize in Iowa State (Da Silva, 2010), Scots pine seedlings in Palencia (Yang et al, 2012), Peach in California (Machon et al, 2006 and apple in China (Ju et al, 2014;Wang et al, 2018). After Fusarium contacts the host plant, it initiates physiological and biochemical reactions inside the strain through processes such as surface molecular interaction and signal transmission, and produces metabolites that act on the plant body to cause pathogenic effects.…”

Section: Discussionmentioning

confidence: 99%

“…So for, F. moniliforme also have been shown to be pathogenic on forest nurseries in Spain (Pinto et al, 2006), Sorghum in India, Maize in Iowa State (Da Silva, 2010), Scots pine seedlings in Palencia (Yang et al, 2012), Peach in California (Machón et al, 2006) and apple in China (Ju et al, 2014). F. moniliforme (F. verticillioides), F. oxysporum, and F. proliferatum isolated from replanted apple soil near the Bohai Bay region in China were highly pathogenic to seedlings of Malus hupehensis in our laboratory research cooperation studies (Yin ., 2017;Wang et al, 2018;Wang et al, 2018). Phloridzin is a characteristic dihydrochalcone of the phenolic compounds produced by apple seedlings (Gosch et al, 2010;Hofmann et al, 2009;Petkovsek et al, 2007Petkovsek et al, , 2011, and phloridzin in the root exudates of Malus x domestica Borkh.…”

Section: Introductionmentioning

confidence: 99%

ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers

Zhang

Jiang

Liu

et al. 2020

Preprint

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Apple replant disease (ARD) has been reported from all major fruit-growing regions of the world, and is often caused by biotic factors (pathogen fungi) and abiotic factors (phenolic compounds). In order to clarify the proteomic differences of Fusarium moniliforme under the action of phloridzin, and to explore the potential mechanism of F. moniliforme as the pathogen of ARD, the role of Fusarium spp in ARD was further clarified. In this paper, the quantitative proteomics method iTRAQ analysis technology was used to analyze the proteomic differences of F. moniliforme before and after phloridzin treatment. A total of 4535 proteins were detected, and 293 proteins were found with more than 1.2 times (P<0.05) differences. In-depth data analysis revealed that differentially expressed proteins were influenced a variety of cellular processes, particularly metabolic processes. Among these metabolic pathways, a total of 8 significantly enriched KEGG pathways were identified with at least 2 affiliated proteins with different abundance in conidia and mycelium. Functional pathway analysis indicated that 46 up-regulated proteins were mainly distributed in amino sugar, nucleotide sugar metabolism, glycolysis/ gluconeogenesis and phagosome pathways. The results deepened our understanding of the proteome in F. moniliforme in response to phloridzin inducers and provide a basis for further exploration for improving the efficiency of the fungi as biocontrol agents to control ARD.

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“…have been shown to be pathogenic [3,6,[32][33][34][35][36][37][38][39]. And F. moniliforme have also been shown to be pathogenic on forest nurseries in Spain [15], Sorghum in India, Maize in Iowa State [16], Scots pine seedlings in Palencia [17], Peach in California [18] and apple in China [19,21]. After Fusarium contacts the host plant, it initiates physiological and biochemical reactions inside the strain through processes such as surface molecular interaction and signal transmission, and produces metabolites that act on the plant body to cause pathogenic effects.…”

Section: Discussionmentioning

confidence: 99%

ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers

Zhang

Jiang

Liu

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Apple replant disease (ARD) has been reported from all major fruit-growing regions of the world, and is often caused by biotic factors (pathogen fungi) and abiotic factors (phenolic compounds). In order to clarify the proteomic differences of Fusarium moniliforme under the action of phloridzin, and to explore the potential mechanism of F. moniliforme as the pathogen of ARD, the role of Fusarium spp in ARD was further clarified.Methods: In this paper, the quantitative proteomics method iTRAQ analysis technology was used to analyze the proteomic differences of F. moniliforme before and after phloridzin treatment. The differentially expressed protein was validated by qRT-PCR analysis.Results: A total of 4535 proteins were detected, and 293 proteins were found with more than 1.2 times (P<0.05) differences. In-depth data analysis revealed that 59 proteins were found with more than 1.5 times (P<0.05) differences, and most proteins were consistent with the result of qRT-PCR. Differentially expressed proteins were influenced a variety of cellular processes, particularly metabolic processes. Among these metabolic pathways, a total of 8 significantly enriched KEGG pathways were identified with at least 2 affiliated proteins with different abundance in conidia and mycelium. Functional pathway analysis indicated that up-regulated proteins were mainly distributed in amino sugar, nucleotide sugar metabolism, glycolysis/ gluconeogenesis and phagosome pathways.Conclusions: This study is the first to perform quantitative proteomic investigation by iTRAQ labeling and LC-MS/MS to identify differentially expressed proteins in F. moniliforme under phloridzin conditions. The results confirmed that F. moniliforme presented a unique protein profile that indicated the adaptive mechanisms of this species to phloridzin environments. The results deepened our understanding of the proteome in F. moniliforme in response to phloridzin inducers and provide a basis for further exploration for improving the efficiency of the fungi as biocontrol agents to control ARD.

show abstract

Analysis of the Fungal Community in Apple Replanted Soil Around Bohai Gulf

Cited by 73 publications

References 26 publications

ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers

ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers

ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers

ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers

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