Analysis of the functional activity of the 1.4-kb 5′-region of the rice actin 1 gene in stable transgenic plants of maize (Zea mays L.)

Zhong, Heng; Zhang, Shibo; Warkentin, D.; Sun, Baolin; Wu, Tsung‐Tsong; Wü, Ray; Sticklen, Mariam B.

doi:10.1016/0168-9452(96)04369-5

Cited by 24 publications

(6 citation statements)

References 23 publications

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“…4). High activity of the Act1 5 H region in actively dividing tissues of transgenic Gladiolus is consistent with previous reports for rice, maize (Zhong et al, 1996), wheat (Nehra et al, 1994) and tritordeum (Barcelo et al, 1994). In roots of transgenic rice plants the most intense uidA staining was at the origin of secondary root formation, and root meristems also showed relatively strong GUS activity.…”

Section: Discussionsupporting

confidence: 89%

Effect of the cauliflower mosaic virus 35S, actin, and ubiquitin promoters on UidA expression from a bar-uidA fusion gene in transgenic Gladiolus plants

Kamo

Blowers²,

McElroy³

2000

In Vitro Cell.Dev.Biol.-Plant

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Tissue-specific patterns and levels of gene expression were characterized in transgenic Gladiolus plants that contained the phosphinothricin acetyltransferase (bar)-b-glucuronidase (uidA) fusion gene under transcriptional control of the promoter from either the cauliflower mosaic virus 35S (CaMV 35S), duplicated CaMV 35S (2 Â CaMV 35S), rice actin (Act1), or Arabidopsis ubiquitin (UBQ3) promoters. The bar gene confers resistance to phosphinothricin (PPT)-containing herbicides and allowed selection of transgenic cells. The b-glucuronidase gene encoded by the uidA locus of E. coli functioned as a reporter gene. Maximum levels of b-glucuronidase (GUS) activity in leaves were 173, 112, 50, and 10 nmoles 4-methylumbelliferone h Ϫ1 mg Ϫ1 protein for transgenic plants with the bar-uidA fusion gene under the control of the CaMV 35S, 2 Â CaMV 35S, UBQ3, and Act1 promoters, respectively. There was frequently considerable variability in GUS activity between the leaves of a single plant, and levels of uidA expression varied between independently transformed plants for each promoter. Callus derived from transgenic plants showed much less variation in GUS expression than leaves. The mean level of GUS activity was significantly higher (over 3Â) for transgenic lines of callus containing the CaMV 35S as compared to the UBQ3 promoter, and this confirmed the higher (2Â) level of GUS activity in leaves of plants with the CaMV 35S promoter as compared to the UBQ3 promoter. Tissue-specific patterns of uidA expression were determined by histochemical staining. Leaves 5-6 cm long from plants with any of the four promoters tested exhibited uidA expression primarily in the vasculature. Under all four promoters uidA was expressed more frequently in root tips as compared to leaves.

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Section: Discussionsupporting

confidence: 89%

Effect of the cauliflower mosaic virus 35S, actin, and ubiquitin promoters on UidA expression from a bar-uidA fusion gene in transgenic Gladiolus plants

Kamo

Blowers²,

McElroy³

2000

In Vitro Cell.Dev.Biol.-Plant

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“…The differentially expressed genes and differentially abundant metabolites in the same treatment were simultaneously mapped to the KEGG pathway map [ 82 ]. A histogram was drawn to show the degree of enrichment of pathways associated with both differential metabolites and differential genes.…”

Section: Methodsmentioning

confidence: 99%

Alpha-Linolenic Acid Mediates Diverse Drought Responses in Maize (Zea mays L.) at Seedling and Flowering Stages

Zhou

2022

Molecules

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Water shortage caused by long-term drought is one of the most serious abiotic stress factors in maize. Different drought conditions lead to differences in growth, development, and metabolism of maize. In previous studies, proteomics and genomics methods have been widely used to explain the response mechanism of maize to long-term drought, but there are only a few articles related to metabolomics. In this study, we used transcriptome and metabolomics analysis to characterize the differential effects of drought stress imposed at seedling or flowering stages on maize. Through the association analysis of genes and metabolites, we found that maize leaves had 61 and 54 enriched pathways under seedling drought and flowering drought, respectively, of which 13 and 11 were significant key pathways, mostly related to the biosynthesis of flavonoids and phenylpropanes, glutathione metabolism and purine metabolism. Interestingly, we found that the α-linolenic acid metabolic pathway differed significantly between the two treatments, and a total of 10 differentially expressed genes and five differentially abundant metabolites have been identified in this pathway. Some differential accumulation of metabolites (DAMs) was related to synthesis of jasmonic acid, which may be one of the key pathways underpinning maize response to different types of long-term drought. In general, metabolomics provides a new method for the study of water stress in maize and lays a theoretical foundation for drought-resistant cultivation of silage maize.

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“…Three cassettes that harboured a non‐maize codon‐optimized version (Chiu et al. , 1996) were assembled under the control of either the 35S CaMV (Benfey and Chua, 1990), rice actin promoter, coupled with a 5′‐intron (Zhong et al. , 1996) or the 1.7 kb maize C4 PepC promoter.…”

Section: Methodsmentioning

confidence: 99%

Transgenic maize lines with cell‐type specific expression of fluorescent proteins in plastids

Sattarzadeh¹,

Fuller²,

Moguel³

et al. 2010

Plant Biotechnology Journal

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SummaryPlastid number and morphology vary dramatically between cell types and at different developmental stages. Furthermore, in C4 plants such as maize, chloroplast ultrastructure and biochemical functions are specialized in mesophyll and bundle sheath cells, which differentiate acropetally from the proplastid form in the leaf base. To develop visible markers for maize plastids, we have created a series of stable transgenics expressing fluorescent proteins fused to either the maize ubiquitin promoter, the mesophyll-specific phosphoenolpyruvate carboxylase (PepC) promoter, or the bundle sheath-specific Rubisco small subunit 1 (RbcS) promoter. Multiple independent events were examined and revealed that maize codon-optimized versions of YFP and GFP were particularly well expressed, and that expression was stably inherited. Plants carrying PepC promoter constructs exhibit YFP expression in mesophyll plastids and the RbcS promoter mediated expression in bundle sheath plastids. The PepC and RbcS promoter fusions also proved useful for identifying plastids in organs such as epidermis, silks, roots and trichomes. These tools will inform future plastid-related studies of wild-type and mutant maize plants and provide material from which different plastid types may be isolated.

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Analysis of the functional activity of the 1.4-kb 5′-region of the rice actin 1 gene in stable transgenic plants of maize (Zea mays L.)

Cited by 24 publications

References 23 publications

Effect of the cauliflower mosaic virus 35S, actin, and ubiquitin promoters on UidA expression from a bar-uidA fusion gene in transgenic Gladiolus plants

Effect of the cauliflower mosaic virus 35S, actin, and ubiquitin promoters on UidA expression from a bar-uidA fusion gene in transgenic Gladiolus plants

Alpha-Linolenic Acid Mediates Diverse Drought Responses in Maize (Zea mays L.) at Seedling and Flowering Stages

Transgenic maize lines with cell‐type specific expression of fluorescent proteins in plastids

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