Analysis of the Function of the Photoreceptors Phytochrome B and Phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana

Fernández, Aurora Piñas; Gil, Patricia; Valkai, Ildikó; Nagy, Ferenc; Schäfer, Eberhard

doi:10.1093/pcp/pci073

Cited by 11 publications

(5 citation statements)

References 31 publications

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“…species, natural accessions or ecotypes) which may have been exposed to different selective pressures will likely evolve specific phytochrome‐dependent mechanisms to respond to light, even though the responses are initiated by the same Pr and Pfr biochemistry (Dudley and Schmitt ; Botto and Smith ; Chitwood et al ). Consistent to this notion, Fernandez et al () reported the functional divergence between Arabidopsis and N. tabacum PHYB‐clade of apoproteins found at the protein level in the control of hypocotyl elongation in N. plumbaginifolia . These authors used cross‐species complementation tests, and showed that while NtPHYB:GFP and AtPHYB:GFP could recover the hypocotyl phenotype of both AtphyB ( phyB‐9) and NpphyB ( hlg ) loss‐of‐function, AtPHYD:GFP could only rescue NpphyB , and not AtphyB , loss‐of‐function.…”

Section: Discussionmentioning

confidence: 72%

Functional specialization of Nicotiana attenuata phytochromes in leaf development and flowering time

Fragoso

Kim

et al. 2017

JIPB

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Phytochromes mainly function in photoautotrophic organisms to adjust growth in response to fluctuating light signals. The different isoforms of plant phytochromes often display both conserved and divergent roles, presumably to fine-tune plant responses to environmental signals and optimize fitness. Here we describe the distinct, yet partially redundant, roles of phytochromes NaPHYA, NaPHYB1 and NaPHYB2 in a wild tobacco species, Nicotiana attenuata using RNAi-silenced phytochrome lines. Consistent with results reported from other species, silencing the expression of NaPHYA or NaPHYB2 in N. attenuata had mild or no influence on plant development as long as NaPHYB1 was functional; whereas silencing the expression of NaPHYB1 alone strongly altered flowering time and leaf morphology. The contribution of NaPHYB2 became significant only in the absence of NaPHYB1; plants silenced for both NaPHYB1 and NaPHYB2 largely skipped the rosette-stage of growth to rapidly produce long, slender stalks that bore flowers early: hallmarks of the shade-avoidance responses. The phenotyping of phytochrome-silenced lines, combined with sequence and transcript accumulation analysis, suggest the independent functional diversification of the phytochromes, and a dominant role of NaPHYB1 and NaPHYB2 in N. attenuata's vegetative and reproductive development.

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Section: Discussionmentioning

confidence: 72%

Functional specialization of Nicotiana attenuata phytochromes in leaf development and flowering time

Fragoso

Kim

et al. 2017

JIPB

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“…Recent work by Dong et al [ 35 ] demonstrated that different tobacco genotypes differ in the depth of photo-dependent dormancy and that the over-expression of NtPHYB1 prevents the induction of photo-dependent dormancy during seed maturation. Nicotiana species are known to have one PHYA gene and, due to duplications during Solanaceae genome evolution, several PHYB genes [ 35 , 36 , 37 , 38 , 39 ]. These light-sensing proteins regulate seed germination independently from each other, both spatially and temporally [ 17 , 40 , 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Gas-Plasma-Activated Water Impact on Photo-Dependent Dormancy Mechanisms in Nicotiana tabacum Seeds

Grainge

Iza

Leubner‐Metzger

et al. 2022

IJMS

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Seeds sense temperature, nutrient levels and light conditions to inform decision making on the timing of germination. Limited light availability for photoblastic species results in irregular germination timing and losses of population germination percentage. Seed industries are therefore looking for interventions to mitigate this risk. A growing area of research is water treated with gas plasma (GPAW), in which the formed solution is a complex consisting of reactive oxygen and nitrogen species. Gas plasma technology is widely used for sterilisation and is an emerging technology in the food processing industry. The use of the GPAW on seeds has previously led to an increase in germination performance, often attributed to bolstered antioxidant defence mechanisms. However, there is a limited understanding of how the solution may influence the mechanisms that govern seed dormancy and whether photoreceptor-driven germination mechanisms are affected. In our work, we studied how GPAW can influence the mechanisms that govern photo-dependent dormancy, isolating the effects at low fluence response (LFR) and very low fluence response (VLFR). The two defined light intensity thresholds affect germination through different phytochrome photoreceptors, PHYB and PHYA, respectively; we found that GPAW showed a significant increase in population germination percentage under VLFR and further described how each treatment affects key physiological regulators.

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“…Arabidopsis is the sole plant species for which the full set of photoreceptor genes has been characterized (Demarsy and Fankhauser 2009;Inoue et al 2010;Nagatani 2010;Chaves et al 2011). In Solanaceae, photoperception and light signaling have been mainly investigated in tomato, tobacco and potato (Perrotta et al 2000;Weller et al 2001;Fernández et al 2005;Rutitzky et al 2009). High-throughput transcriptomic analyses have demonstrated extensive sequence and functional conservation among phenotypically distinct Solanaceae species potato, tomato, pepper, petunia, tobacco and Nicotiana benthamiana, although species-specific transcripts were also indentified (Rensink et al 2005;Rutitzky et al 2009).…”

Section: Discussionmentioning

confidence: 99%

A reverse genetics approach identifies novel mutants in light responses and anthocyanin metabolism in petunia

Berenschot

Quecini

2013

Physiol Mol Biol Plants

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Flower color and plant architecture are important commercially valuable features for ornamental petunias (Petunia x hybrida Vilm.). Photoperception and light signaling are the major environmental factors controlling anthocyanin and chlorophyll biosynthesis and shadeavoidance responses in higher plants. The genetic regulators of these processes were investigated in petunia by in silico analyses and the sequence information was used to devise a reverse genetics approach to probe mutant populations. Petunia orthologs of photoreceptor, light-signaling components and anthocyanin metabolism genes were identified and investigated for functional conservation by phylogenetic and protein motif analyses. The expression profiles of photoreceptor gene families and of transcription factors regulating anthocyanin biosynthesis were obtained by bioinformatic tools. Two mutant populations, generated by an alkalyting agent and by gamma irradiation, were screened using a phenotype-independent, sequence-based method by high-throughput PCR-based assay. The strategy allowed the identification of novel mutant alleles for anthocyanin biosynthesis (CHALCONE SYNTHASE ) and regulation (PH4), and for light signaling (CONSTANS ) genes.

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Analysis of the Function of the Photoreceptors Phytochrome B and Phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana

Cited by 11 publications

References 31 publications

Functional specialization of Nicotiana attenuata phytochromes in leaf development and flowering time

Functional specialization of Nicotiana attenuata phytochromes in leaf development and flowering time

Gas-Plasma-Activated Water Impact on Photo-Dependent Dormancy Mechanisms in Nicotiana tabacum Seeds

A reverse genetics approach identifies novel mutants in light responses and anthocyanin metabolism in petunia

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