Analysis of the Expression of SELL, ADCY8 and TLR4 in Low Grade Gliomas and Correlation with Prognosis Based on Tumor Subtype Clustering

Abstract

Purpose: To identify a specific group with poor prognosis in LGG(low-grade glioma), analyze the expression of SELL,ADCY8 and TLR4 in LGG, associated pathways and their association with patient outcomes.Material and Methods: Bioinformatics analysis ConsensusClusterPlus cluster analysis was used to identify specific groups with poor prognosis in LGG. By the GSEA algorithm, significantly up-regulated pathway was found. Furthermore, in the TCGA database dataset and CGGA database data, it was found that there were prognostic related genes SELL, ADCY8 and TLR4, high expression was significantly associated with poor prognosis in LGG. Results:In TCGA and CGGA database data, there was a specific group in the LGG database, the prognosis of this group was worse than that of other group (three groups are identified by clustering algorithm), the up-regulated genes in this group are mainly involved in immune cell interaction, immune cell migration, antigen presentation, cytokine pathway and other related pathways. The upregulated genes SELL, ADCY8 and TLR4 in EC1 were lower than that of patients with low expression of TLR4 (SELL p.Value=0.0435, ADCY8 p.value=0.0435, TLR4 p.value=0. 0367).Conclusions: There is a specific group in LGG that has a significantly poor prognosis and the significantly up-regulated tumor immune-related pathway compared with other groups. The high expression of SELL, ADCY8 and TLR4 in LGG were associated with poor prognosis and immune infiltration.