Analysis of the expression of angiotensin II type 1 receptor and VEGF in endometrial adenocarcinoma with different clinicopathological characteristics

Abstract

In Poland, endometrial carcinoma takes second place after breast cancer among all cancers in women and is considered the most common genital cancer. It has been repeatedly reported that angiotensin is involved in the development and invasion of some cancers including breast, ovarian, and pancreatic ones. It is suggested that angiotensin two and its receptors are actively involved in tumour biology in endometrial adenocarcinoma. In the present study, we identify a possible relationship between the expression of AT1-R, AT2-R, ERα, and VEGF and clinicopathological characteristics of primary endometrial adenocarcinoma. We determined the above components both at the mRNA (real-time RT-PCR) and protein levels (Western Blot assay). Our results indicate that in patients with grade G3 adenocarcinoma, the expression of AT1-R significantly decreased in comparison with G1 patients (p = 0.034), but the level of ERα was the highest in G2 and the lowest in G3. Moreover, the level of VEGF mRNA significantly increased between G2 and G3 (p = 0.034). We also noted a significant correlation between the expression of AT1-R and AT2-R in FIGO stage 1 (R (s) = 0.9636; p = 0.0001) and that of AT2-R and VEGF (R (s) = 0.5377; p = 0.005). In grade G1 and G2 carcinoma, a significant correlation was also found between the expression of AT1-R and AT2-R (R (s) = 0.9924; p = 0.0001; R (s) = 0.8717, p = 0.0005, respectively), but in grade G1, a negative correlation was observed between AT1-R and VEGF (R (s) = -0.8945, p = 0.0005). Further studies are required to clarify the biological function of the angiotensin receptor in regulating VEGF expression in endometrial carcinoma.

The evaluation of involvement of angiotensin II, its receptors, and androgen receptor in endometrial cancer

“…The highest expression was observed in G1 stage (well differentiated) and the lowest in G3 stage (poorly differentiated). The reason of the dominance of AT1 in G1 stage in comparison with G2 and G3 stages may be resulted from the involvement of Ang in the development of primary cancer that is related to angiogenesis [14]. Yang et al analyzed the correlation between AT1 and proliferation of Ishikawa cells (EC cell line).…”

Angiotensin modulates human mammary epithelial cell motility

“…7 Most components of the RAS are expressed locally in a large variety of tissues and tumours, including mammary gland and breast cancers. 3,[7][8][9] AngII is known to stimulate proliferation of breast cancer cells, but it may also have other activities. One possibility is that it may be involved in breast cancer progression.…”

3'-UTR polymorphism (rs10434) in the VEGF gene is associated with B-CLL in a Chinese population

“…Breast and gynecologic cancer are among the best-known malignancies involving lymphangiogenesis, which is the recruitment of blood and lymphatic vessels, to a growing tumor (Schoppmann et al, 2002). This has been confirmed by previous studies demonstrating that VEGF plays an important role in the development of breast (Berezov et al, 2009), reproductive organ (Mazurek et al, 2004;Dobrzycka et al, 2011;Piastowska-Ciesielska et al, 2012), and ovarian cancer (Hefler et al, 2006;Sadłecki et al, 2011), as well as lung (Carrillo-de Santa Pau et al, 2010), colon (Bunger, 2011), and gastric (Hălmaciu et al, 2012) cancer.…”