Analysis of the expression and the first exon of aromatase mRNA in monkey brain

Yamada-Mouri, Naoko; Hirata, Shuji; Hayashi, Michihiro; Kato, Junzo

doi:10.1016/0960-0760(95)00157-u

Cited by 31 publications

(10 citation statements)

References 25 publications

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“…In general, the data obtained in the present study confirm and expand previous work in monkeys that used regional dissections and punch microdissections to map the distribution of both P450 AROM activity and mRNA expression (Roselli et al, 1987;Roselli and Resko, 1989;Yamada-Mouri et al, 1995;Abdelgadir et al, 1997;Resko et al, 2000a). In agreement with our earlier micropunch study of the rhesus monkey brain (Roselli et al, 1987), P450 AROM mRNA was found throughout the rostral diencephalon, with the highest expression in the MPO, AHA, BST, and VMN.…”

Section: Comparison With Previous Studies Of Aromatase and Androgen Rsupporting

confidence: 94%

Anatomic relationships between aromatase and androgen receptor mRNA expression in the hypothalamus and amygdala of adult male cynomolgus monkeys

Roselli

Klosterman

Resko

2001

J of Comparative Neurology

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This study mapped the regional locations of cells expressing cytochrome P450 aromatase (P450AROM) and androgen receptor (AR) mRNAs in the adult male macaque hypothalamus and amygdala by in situ hybridization histochemistry using monkey-specific cRNA probes. High densities of P450AROM and AR mRNA-containing neurons were observed in discrete hypothalamic areas involved in the regulation of gonadotropin secretion and reproductive behavior. P450AROM mRNA-containing neurons were most abundant in the medial preoptic nucleus, bed nucleus of the stria terminalis, and anterior hypothalamic area, whereas AR mRNA-containing neurons were most numerous in the ventromedial nucleus, arcuate nucleus, and tuberomamillary nucleus. Moderate to heavily labeled P450AROM mRNA-containing cells were present in the cortical and medial amygdaloid nuclei, which are known to have strong reciprocal inputs with the hypothalamus. Heavily labeled P450AROM mRNA-containing cells were found in the accessory basal amygdala nucleus, which projects to the cingulate cortex and hippocampus, areas that are important in the expression of emotional behaviors and memory processing. In contrast to P450AROM, the highest density of AR mRNA labeling in the temporal lobe was associated with the cortical amygdaloid nucleus and the pyramidal cells of the hippocampus. All areas that contained P450AROM mRNA-expressing cells also contained AR mRNA-expressing cells, but there were areas in which AR mRNA was expressed but not P450AROM mRNA. The apparent relative differences in the expression of P450AROM and AR mRNA-containing neurons within the monkey brain suggests that T acts through different signaling pathways in specific brain areas or within different cells from the same region.

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Section: Comparison With Previous Studies Of Aromatase and Androgen Rsupporting

confidence: 94%

Anatomic relationships between aromatase and androgen receptor mRNA expression in the hypothalamus and amygdala of adult male cynomolgus monkeys

Roselli

Klosterman

Resko

2001

J of Comparative Neurology

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“…In fact, our work demonstrates the use of additional promoters, and for the first time to our knowledge, three aromatase transcripts have been observed in the germ cells studied (G-PL, PS, and RS): one is derived from promoter PII, and another one is from promoter PI.f, which is the main promoter responsible for aromatase expression in the brain (Yamada-Mouri et al 1996, Kato et al 1997. Although some transcripts issuing from PI.f have been detected in the testis of monkey (Yamada-Mouri et al 1995), rat (Yamada-Mouri et al 1996), and mouse (Golovine et al 2003), it is the first time that they are described in rat germ cells; they are more expressed in PS and RS than in a mixture of spermatogonia and preleptotene spermatocytes. Indeed, some samples of G-PL have very low levels of specific transcripts issuing from PI.f, whereas other samples do not express these transcripts.…”

Section: Discussionmentioning

confidence: 99%

Three promoters PII, PI.f, and PI.tr direct the expression of aromatase (cyp19) gene in male rat germ cells

Silandre¹,

Delalande²,

Durand³

et al. 2007

Journal of Molecular Endocrinology

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Aromatase is the key enzyme responsible for the irreversible transformation of androgens into estrogens. It is encoded by the cyp19 gene and is expressed in the mammalian testis under the control of the proximal promoter PII. Since both somatic and germ cells contain a biologically active aromatase, we looked for the existence of other promoters that may direct the expression of aromatase in adult rat germ cells. Besides the promoter II, we have shown the presence of transcripts derived from the brain promoter PI.f in spermatogonia-preleptotene spermatocytes (G-PL), pachytene spermatocytes (PS), and round spermatids (RS). A new aromatase cDNA has been isolated by 5 0 -rapid amplification of cDNA ends that we named I.tr (testis rat). The I.tr transcripts are found in all the testicular cell populations studied with a greater expression in PS. Because of the utilization of these three promoters in the adult rat testis, we studied their putative involvement according to the age. At 10 days, aromatase expression was very low and then strongly increased between 10 and 20 days, with a preferential activation of PII and PI.tr. Transcripts coming from PI.f were found starting from 20 days onwards. The new promoter PI.tr, localized between promoters PI.f and PII, is devoid of a TATA box but contains a transcriptional initiator (Inr) and putative regulatory sequences. Therefore, the identification of the specific trans-activating factors should bring some enlightenments to understand the regulation of these three promoters in germ cells especially according to their stage of development.

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“…Exon 1f-containing mRNA was originally cloned from brain tissues and has been described as brain specific (21,(41)(42)(43). However, several aromatase expression studies have shown a vast tissue distribution for many aromatase mRNA species (7,10,24), suggesting that the tissue specificity model for aromatase promoter usage may be oversimplified.…”

Section: Discussionmentioning

confidence: 99%

“…The 5Ј-end of clone A17 (bases 1-77; Fig. 1B) was found to be the equine homolog of aromatase exon 1f, often designated as brain specific (21,(41)(42)(43). Clone A17 was designated as a promoter 1f-derived aromatase transcript.…”

Section: Characterization Of Equine Cytochromes P450arom and P45017␣ mentioning

confidence: 99%

Dual Regulation of Promoter II- and Promoter 1f-Derived Cytochrome P450 Aromatase Transcripts in Equine Granulosa Cells during Human Chorionic Gonadotropin-Induced Ovulation: A Novel Model for the Study of Aromatase Promoter Switching

Boerboom¹

1999

Endocrinology

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Estradiol biosynthesis is a key biochemical trait of developing follicles. To study its regulation in equine follicles, the objectives of this study were to clone and determine the structure of equine cytochrome P450 aromatase (P450AROM), and characterize the regulation of P450AROM and P450 17␣-hydroxylase/C17-20 lyase (P45017␣) messenger RNAs (mRNAs) in vivo in equine preovulatory follicles isolated during hCGinduced ovulation. Two distinct P450AROM complementary DNAs (cDNAs) were isolated from an equine preovulatory follicle cDNA library. One clone was 2682 bp in length and included 115 bp of 5Ј-untranslated region (UTR), 1509 bp of open reading frame encoding a well conserved 503-amino acid protein, and 1058 bp of 3Ј-UTR. Its 5Ј-most region represented the equine homolog of exon 1f, previously designated brain specific. The other cDNA clone encoded a truncated protein and contained a distinct 5Ј-UTR characteristic of transcripts derived from promoter II, previously identified as the predominant ovarian mRNA. Northern blot analyses were performed using preovulatory follicles obtained during estrus between 0 -39 h after the administration of hCG and with corpora lutea isolated on day 8 of the estrous cycle (day 0 ϭ day of ovulation). The results showed a biphasic regulation of P450AROM mRNA expression: levels were highest in follicles at 0 h post-hCG, decreased significantly during the ovulatory process at 12 and 24 h (P Ͻ 0.05), and increased again between 30 -39 h post-hCG and in corpora lutea. When oligonucleotides specific for P450AROM mRNA variants were used as probes, a novel switching phenomenon was observed. Promoter II-derived transcripts accounted for the message present in follicles at 0 h post-hCG and in corpora lutea, whereas promoter 1f-derived mRNA was expressed exclusively during the ovulatory process (30 -39 h post-hCG). Levels of P45017␣ mRNA were high in follicles at 0 h, but significantly decreased after hCG treatment (P Ͻ 0.05), with lowest levels in follicles at 36 and 39 h post-hCG and in corpora lutea. Northern blots performed on isolated cellular preparations revealed that P450AROM and P45017␣ transcripts were localized exclusively in granulosa cells and theca interna, respectively. Equine aromatase promoters II and 1f were cloned from a genomic library, and putative transcription start sites were characterized by primer extension assays. Sequence analyses identified distinct potential regulatory elements in each promoter. Thus, this study identifies a novel aromatase promoter-switching phenomenon in equine granulosa cells during follicular luteinization and provides a new model in which aromatase promoter switching is induced in vivo. (Endocrinology 140: 4133-4141, 1999)

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Analysis of the expression and the first exon of aromatase mRNA in monkey brain

Cited by 31 publications

References 25 publications

Anatomic relationships between aromatase and androgen receptor mRNA expression in the hypothalamus and amygdala of adult male cynomolgus monkeys

Anatomic relationships between aromatase and androgen receptor mRNA expression in the hypothalamus and amygdala of adult male cynomolgus monkeys

Three promoters PII, PI.f, and PI.tr direct the expression of aromatase (cyp19) gene in male rat germ cells

Dual Regulation of Promoter II- and Promoter 1f-Derived Cytochrome P450 Aromatase Transcripts in Equine Granulosa Cells during Human Chorionic Gonadotropin-Induced Ovulation: A Novel Model for the Study of Aromatase Promoter Switching

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