Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography

Zhang, Yan; Chen, Jiping; Zhang, Chengqi; Wu, Wenzhong; Liang, Xinmiao

doi:10.1016/j.jsbmb.2004.10.022

Cited by 29 publications

(28 citation statements)

References 27 publications

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“…There was a conclusion that isoflavonoids in P. radix were less potent than the Thai P. mirifica [29]. In the other study, the crude extract and sub-fraction of the Chinese P. lobata showed estrogenic activity in a recombinant yeast screening assay [30].…”

Section: Introductionmentioning

confidence: 88%

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Major isoflavonoid contents of the phytoestrogen rich-herb Pueraria mirifica in comparison with Pueraria lobata

Cherdshewasart

Subtang

Dahlan

2007

Journal of Pharmaceutical and Biomedical Analysis

129

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Section: Introductionmentioning

confidence: 88%

“…Only daidzein of P. lobata was found at higher concentration than the mean value of the P. mirifica population. It was also noted that the reported isoflavonoid contents in P. lobata also varied with the location of sample collection [29][30].…”

Section: Inter-provincial Studymentioning

confidence: 99%

Major isoflavonoid contents of the phytoestrogen rich-herb Pueraria mirifica in comparison with Pueraria lobata

Cherdshewasart

Subtang

Dahlan

2007

Journal of Pharmaceutical and Biomedical Analysis

129

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“…This is of great concern as Avlimil is marketed to a large proportion of women with sexual discomfort caused by hormonal changes, menopause and aging. Avlimil, as many dietary supplements, is a mixture of water and solvent extracts from different herbs including Pueraria montana (kudzu root extract), Trifolium pratense (red clover extract), Glycyrrhiza glabra (licorice root) and Actaea racemosa (black cohosh root), among others, which contain a variety of bioactive phytochemicals with known oestrogenic, antioestrogenic and /or anti-proliferative activity (Boue et al, 2003;Burdette et al, 2002;Zava and Duwe, 1997;Zhang et al, 2005). It is possible that this botanical preparation, consumed in high or low amounts, could modulate breast cancer tumour growth in postmenopausal women.…”

Section: Properties Of Phytoestrogenesmentioning

confidence: 99%

Phytoestrogens and breast cancer: a complex story

2008

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Genistein is an isoflavone with oestrogenic activity that is present in a variety of soy products as a constituent of complex mixtures of bioactive compounds, whose matrix profiles play an important role in determining the overall oestrogenic bioactivity of genistein. We review data on how the profile of soy bioactive compounds can modulate genistein-stimulated oestrogen-dependent tumour growth. Our research has focused on the effects of dietary genistein on the growth of oestrogen (E)-dependent mammary tumours both in vitro and in vivo. Genistein enhances the proliferation of E-dependent human breast cancer tumour growth. In a similar manner, dietary genistein stimulates tumour growth in the chemically-induced (NMU) mammary cancer rodent model. Genistin, the glycoside of genistein, simulates growth similar to that of genistein and withdrawal of either genistein or genistin results in tumour regression. The extent of soy processing modulates the effects of dietary genistein in vivo as soy protein isolate, a highly purified and widely used source of protein that is processed to contain low, medium, and high amounts of isoflavones, stimulate the growth of the E-dependent mammary tumours in a dose dependent manner. In contrast to the more purified diets, studies with soy flour of equivalent genistein levels did not stimulate the growth of E-dependent breast cancer tumours in vivo. However, the size of these tumours also did not regress as is observed in control groups in which oestrogen and genistein have been withdrawn. The expression of the oestrogen-target genes of pS2, progesterone receptor, and cyclin D1 correlates with the growth of E-dependent tumours and has been consistently observed to be induced in response to treatment with dietary genistein. To evaluate whether dietary genistein interacts with current anti-oestrogen breast cancer therapies such as tamoxifen (TAM), we implanted E-dependent tumours into ovariectomized athymic mice and administered oestradiol, oestradiol plus TAM, or oestradiol, TAM, and dietary genistein. In these studies dietary genistein was able to negate the inhibitory effect of TAM on E-stimulated tumour growth. In summary, genistein can act as an oestrogen agonist resulting in proliferation of E-dependent human breast cancer tumours in vivo and its activity can be modulated by the presence of other bioactive components in complex soy foods. Additionally, dietary genistein can negate the inhibitory effects of TAM on E-stimulated growth of MCF-7 cell tumours implanted into ovariectomized athymic mice.

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“…(1) 유효 성분 갈근의 isoflavone 함량은 0.5-16%로 다양하고, 주 요한 isoflavone으로는 puerarin, daidzin, daidzein, genistin, genistein이 있으며, 이 중 에스트로겐 활성은 31% 정도의 estradiol 효과를 보이는 daidzein 함량과 직접적으로 관련이 있다 13) . 갈근의 daidzein 함량은 콩 보다 높고 총 isoflavone 양은 콩의 30배에 해당하며 14) , 부위별 daidzein은 뿌리 > 줄기 > 잎 > 꽃 > 종자 순서 로 함량이 많고 에스트로겐 활성도 같은 순서이다 15) .…”

Section: 갈근 (Pueraiae Radix)unclassified