Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells

Saleh, Farid H.; Asfar, Sami; Oteifa, Medhat; Al-Saleh, N

doi:10.1186/1472-6882-14-322

Cited by 21 publications

(18 citation statements)

References 45 publications

(39 reference statements)

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“…From an immune perspective, EGCG significantly suppressed IFN-γ production and the proliferation of peripheral blood mononuclear cells in vitro [465]. It was also shown to exert antitumor effects on colorectal cancer cells, at least in part by inhibiting the expression and function of IDO through the suppression of STAT1 activation [466].…”

Section: Low Toxicity Approachesmentioning

confidence: 99%

A multi-targeted approach to suppress tumor-promoting inflammation

Samadi

Bilsland

Georgakilas

et al. 2015

Seminars in Cancer Biology

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Cancers harbor significant genetic heterogeneity and patterns of relapse following many therapies are due to evolved resistance to treatment. While efforts have been made to combine targeted therapies, significant levels of toxicity have stymied efforts to effectively treat cancer with multi-drug combinations using currently approved therapeutics. We discuss the relationship between tumor-promoting inflammation and cancer as part of a larger effort to develop a broad-spectrum therapeutic approach aimed at a wide range of targets to address this heterogeneity. Specifically, macrophage migration inhibitory factor, cyclooxygenase-2, transcription factor nuclear factor-kappaB, tumor necrosis factor alpha, inducible nitric oxide synthase, protein kinase B, and CXC chemokines are reviewed as important antiinflammatory targets while curcumin, resveratrol, epigallocatechin gallate, genistein, lycopene, and anthocyanins are reviewed as low-cost, low toxicity means by which these targets might all be reached simultaneously. Future translational work will need to assess the resulting synergies of rationally designed antiinflammatory mixtures (employing low-toxicity constituents), and then combine this with similar approaches targeting the most important pathways across the range of cancer hallmark phenotypes.

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Section: Low Toxicity Approachesmentioning

confidence: 99%

A multi-targeted approach to suppress tumor-promoting inflammation

Samadi

Bilsland

Georgakilas

et al. 2015

Seminars in Cancer Biology

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“…PBMC viability in culture medium alone was in the range of 99-100%, BTMFA treatment in the range of 90-100% viability (Figure 3(A1-A3)), and BTMFB treated in the range of 84-86% viability after 48 h (Figure 3(B1-B3)). Pure catechin treatment with PBMC revealed the decreasing cell viability of unstimulated PBMCs [31,32]. Similarly, other studies have also reported that T cell proliferation was inhibited by catechin through inhibiting T cell division and cell cycle progression in a dose-dependent manner in vitro [33].…”

Section: Effect Of Biotransformed Catechin Metabolites On the Cell Pomentioning

confidence: 80%

Colonic Bacteria-Transformed Catechin Metabolite Response to Cytokine Production by Human Peripheral Blood Mononuclear Cells

et al. 2019

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Human gut microbes are a profitable tool for the modification of food compounds into biologically active metabolites. The biological properties of catechins have been extensively investigated. However, the bioavailability of catechin in human blood plasma is very low. This study aimed to determine the biotransformed catechin metabolites and their bioactive potentials for modulating the immune response of human peripheral blood mononuclear cells (PBMCs). Biotransformation of catechin was carried out using in-vitro gut microbial biotransformation method, the transformed metabolites were identified and confirmed by gas chromatography-mass spectrometry (GC–MS) and high-performance liquid chromatography-mass spectrometry (HPLC–MS). Present observations confirmed that the catechin was biotransformed into 11 metabolites upon microbial dehydroxylation and C ring cleavage. Further, immunomodulatory potential of catechin metabolites was analyzed in peripheral blood mononuclear cells (PBMCs). We found up-regulation of anti-inflammatory cytokine (IL-4, IL-10) and down-regulation of pro-inflammatory (IL-16, IL-12B) cytokine may be due to Th2 immune response. In conclusion, biotransformed catechin metabolites enhance anti-inflammatory cytokines which is beneficial for overcoming inflammatory disorders.

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“…Kaempferol inhibited the activation and proliferation of mouse T lymphocytes in response to ConA, arresting cell cycle at S phase and G2/M in vitro (Mu et al 2009). In humans, green tea catechin EGCG significantly suppressed in vitro lymphocyte proliferation in response to stimulation and IFN gamma production (Saleh et al 2014). Recent studies have shown that green tea catechin, EGCG, affects the differentiation of najve T cells into different effector subsets (Pae and Wu 2013).…”

Section: Discussionmentioning

confidence: 99%

Spleen content of selected polyphenols, splenocytes morphology and function in mice fed Rhodiola kirilowii extracts during pregnancy and lactation

Lewicki¹,

Stankiewicz²,

Skopińska-Różewska³

et al. 2015

Polish Journal of Veterinary Sciences

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The genus Rhodiola (Crassulaceae) consists of many species, growing mainly in Asia and traditionally used as adaptogens and anti-inflammatory drugs. In order to elaborate herbal immunostimulator which could be safely given to pregnant women, we performed a study on immunotropic effects of feeding pregnant and lactating mice Rhodiola kirilowii extracts. This paper presents the results of the first part of our study -spleen content of selected polyphenols, spleen cellularity, splenocytes phenotype and their response to mitogens. Experiments were performed on adult inbred females of Balb/c strain, mated with adult males. Females, since copulatory plug was noted, up to the 28-th day after delivery were fed daily with 20 mg/kg b.m. water (RKW) or hydro-alcoholic (RKW-A) extracts of Rhodiola kirilowii. Results: 1. Significantly lower proportion of pregnant mice in experimental groups than in the control. 2. Cellularity of spleen and flavonol quercetin spleen concentration were significantly lower in experimental groups in comparison to the controls. 3. Flavanols ( (+)-catechin and epicatechin) levels were significantly higher in the spleens of experimental mice than in the controls. 4. Positive correlation between spleen cellularity and quercetin, and negative correlation between spleen cellularity and epicatechin content were observed. 5. Spleen mass and spleen lymphocytes phenotype and proliferation in RKW and RKW-A fed mice did not differ from the control. These results, together with suspicion of some embryo-toxicity, are worrying and eliminate the possibility of use Rhodiola kirilowii extracts for long-term treatment in pregnant females.

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Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells

Cited by 21 publications

References 45 publications

A multi-targeted approach to suppress tumor-promoting inflammation

A multi-targeted approach to suppress tumor-promoting inflammation

Colonic Bacteria-Transformed Catechin Metabolite Response to Cytokine Production by Human Peripheral Blood Mononuclear Cells

Spleen content of selected polyphenols, splenocytes morphology and function in mice fed Rhodiola kirilowii extracts during pregnancy and lactation

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